可过滤液体饮料中微生物学检验滤膜法和平板计数法的等效性比较分析
|
摘要
比较分析滤膜法和平板计数法在检验可过滤饮料中菌落总数、霉菌和酵母计数以及大肠菌群计数微生物指标是否具有等效性。通过预实验结果,采集可过滤液体饮料样品分别加入定量的枯草芽孢杆菌、白色念珠菌、黑曲霉以及大肠杆菌制备成样品,分别采用滤膜法与平板计数法分别检测菌落总数、霉菌计数、酵母计数以及大肠菌群计数,参照采用ISO 17994:2014《水质-用两种定量方法对比微生物回收率的基本要求》对滤膜法和平板计数法的检测结果,然后进行数据比较分析,以验证两种方法在可过滤液体饮料微生物学检验中的是否具有等效性。结果表明当取样量为100时,滤膜法和平板计数法在检测可过滤液体饮料微生物学检验中具有等效性。滤膜法与平板计数法应用于可过滤饮料中微生物学指标的检验可考虑相互取代。
The aim of this study was to investigate whether there is equivalence between filter membrane method and plate counting method in examination of microbiological indexes of filterable beverage which include total numbers of colony and the count of mold, yeast and coliform. Through pre-experimental results, 100 copies of filterable liquid beverage were collected and then added with quantitative bacterial fluid for examination of total numbers of colony and the count of mold, yeast and coliform by using filter membrane method and plate counting method respectively. After that, comparative analysis on results of the two methods was conducted based on ISO 17994:2014 "Water qualityRequirements for the comparison of the relative recovery of microorganisms by two quantitative methods ", so that the equivalence of the two methods in microbiological examination of filterable liquid beverage could be verified. The results indicated that, filter membrane method and plate counting method have equivalence in microbiological examination of filterable liquid beverage when its sampling quantity reaches 100. Thus, the two methods are alternative when applied to the examination of microbiological indexes of filterable liquid beverage.
The aim of this study was to investigate whether there is equivalence between filter membrane method and plate counting method in examination of microbiological indexes of filterable beverage which include total numbers of colony and the count of mold, yeast and coliform. Through pre-experimental results, 100 copies of filterable liquid beverage were collected and then added with quantitative bacterial fluid for examination of total numbers of colony and the count of mold, yeast and coliform by using filter membrane method and plate counting method respectively. After that, comparative analysis on results of the two methods was conducted based on ISO 17994:2014 "Water qualityRequirements for the comparison of the relative recovery of microorganisms by two quantitative methods ", so that the equivalence of the two methods in microbiological examination of filterable liquid beverage could be verified. The results indicated that, filter membrane method and plate counting method have equivalence in microbiological examination of filterable liquid beverage when its sampling quantity reaches 100. Thus, the two methods are alternative when applied to the examination of microbiological indexes of filterable liquid beverage.
引文
[1]刘国良,朱守一.使用微孔滤膜的微生物检验技术[J].中国医药工业杂志,1975,6:66-69LIU Guo-liang,ZHU Shou-yi.Microbiological inspection techniques using microporous membranes[J].Chinese Journal of Pharmaceuticals,1975,6:66-69
[2]宁培勇,丁津华,徐文体,等.滤膜过滤技术应用研究进展[J].中国消毒学杂志,2006,23(1):63-65NING Pei-yong,DING Jin-hua,XU Wen-ti,et al.Research progress of application of filter membrane filtration technology[J].Chinese Journal of Disinfection,2006,23(1):63-65
[3]张明,姜明洪,吴光虹,等.滤膜法在饮料微生物检验中的应用[J].饮料工业,2011,14(11):43-46ZHANG Ming,JIANG Ming-hong,WU Guang-hong,et al.Application of membrane filtration microbian detection of beveragea[J].Beverage Industry,2011,14(14):43-46
[4]孙娜,刘海洲,张捷,等.滤膜法与国标法用于微生物检测的比较研究[J].化学与生物工程,2008,25(8):70-72SUN Na,LIU Hai-zhou,ZHANG Jie,et al.The comparison research on membrane filter method and encoding standard method for microbial detection[J].Chemistry&Bioengineering,2008,25(8):70-72
[5]GB 4789.2-2016食品安全国家标准食品微生物学检验菌落总数[S]GB 4789.2-2016,National food safety standard food microbiological examination:Aerobic Plate Count[S]
[6]GB 4789.15-2016,食品安全国家标准食品微生物学检验霉菌和酵母计数[S]GB 4789.15-2016,National food safety standard food microbiological examination:emumeration of yeasts and molds[S]
[7]GB 4789.3-2016,食品安全国家标准食品微生物学检验大肠菌群计数[S]GB 4789.3-2016,National food safety standard food microbiological examination:emumeration of coliforms[S]
[8]ISO 17994-2014《水质-用两种定量方法对比微生物回收率的基本要求》[S]ISO 17994-2014 Water quality-requirements for the comparison of the relative recovery of microorganisms by two quantitative methods[S]
[9]武利平,李晓路,刘聪颖,等.两种方法检测水中总大肠菌群和大肠埃希菌的等效性分析[J].卫生研究,2017,46(5):793-796WU Li-ping,LI Xiao-lu,LIU Cong-ying,et al.Equivalence test between trial method and reference method of the total Coliforms and E.Coli in water[J].Journal of Hygiene Research,2017,46(5):793-796
[10]武利平,丁珵,孙宗科.水中菌落总数检测方法的等效性判定[J].环境与健康杂志,2017,34(3):246-248WU Li-ping,DING Cheng,SUN Zong-ke.Equivalence Test between trial method and reference method of the total mumber of colonies in water[J].Journal of Environment and Health,2017,34(3):246-248
[11]孙宗科,高飞,张伟,等.水中大肠菌群检测方法的等效性判断[J].环境与健康杂志,2011,28(4):355-357SUN Zong-ke,GAO Fei,ZHANG Wei,et al.Equivalence test between trial method and reference method of detecting Coliform in water[J].Journal of Environment and Health,2011,28(4):355-357
[12]张俊伟,邹凯伦.微孔滤膜在实验室研究与分析检测中的应用[J].山东化工,2018,47(6):86-88ZHANG Jun-wei,ZOU Kai-lun.Application of microporous membrane in lad research and analysis[J].Shandong Chemical Industry,2018,47(6):86-88
[2]宁培勇,丁津华,徐文体,等.滤膜过滤技术应用研究进展[J].中国消毒学杂志,2006,23(1):63-65NING Pei-yong,DING Jin-hua,XU Wen-ti,et al.Research progress of application of filter membrane filtration technology[J].Chinese Journal of Disinfection,2006,23(1):63-65
[3]张明,姜明洪,吴光虹,等.滤膜法在饮料微生物检验中的应用[J].饮料工业,2011,14(11):43-46ZHANG Ming,JIANG Ming-hong,WU Guang-hong,et al.Application of membrane filtration microbian detection of beveragea[J].Beverage Industry,2011,14(14):43-46
[4]孙娜,刘海洲,张捷,等.滤膜法与国标法用于微生物检测的比较研究[J].化学与生物工程,2008,25(8):70-72SUN Na,LIU Hai-zhou,ZHANG Jie,et al.The comparison research on membrane filter method and encoding standard method for microbial detection[J].Chemistry&Bioengineering,2008,25(8):70-72
[5]GB 4789.2-2016食品安全国家标准食品微生物学检验菌落总数[S]GB 4789.2-2016,National food safety standard food microbiological examination:Aerobic Plate Count[S]
[6]GB 4789.15-2016,食品安全国家标准食品微生物学检验霉菌和酵母计数[S]GB 4789.15-2016,National food safety standard food microbiological examination:emumeration of yeasts and molds[S]
[7]GB 4789.3-2016,食品安全国家标准食品微生物学检验大肠菌群计数[S]GB 4789.3-2016,National food safety standard food microbiological examination:emumeration of coliforms[S]
[8]ISO 17994-2014《水质-用两种定量方法对比微生物回收率的基本要求》[S]ISO 17994-2014 Water quality-requirements for the comparison of the relative recovery of microorganisms by two quantitative methods[S]
[9]武利平,李晓路,刘聪颖,等.两种方法检测水中总大肠菌群和大肠埃希菌的等效性分析[J].卫生研究,2017,46(5):793-796WU Li-ping,LI Xiao-lu,LIU Cong-ying,et al.Equivalence test between trial method and reference method of the total Coliforms and E.Coli in water[J].Journal of Hygiene Research,2017,46(5):793-796
[10]武利平,丁珵,孙宗科.水中菌落总数检测方法的等效性判定[J].环境与健康杂志,2017,34(3):246-248WU Li-ping,DING Cheng,SUN Zong-ke.Equivalence Test between trial method and reference method of the total mumber of colonies in water[J].Journal of Environment and Health,2017,34(3):246-248
[11]孙宗科,高飞,张伟,等.水中大肠菌群检测方法的等效性判断[J].环境与健康杂志,2011,28(4):355-357SUN Zong-ke,GAO Fei,ZHANG Wei,et al.Equivalence test between trial method and reference method of detecting Coliform in water[J].Journal of Environment and Health,2011,28(4):355-357
[12]张俊伟,邹凯伦.微孔滤膜在实验室研究与分析检测中的应用[J].山东化工,2018,47(6):86-88ZHANG Jun-wei,ZOU Kai-lun.Application of microporous membrane in lad research and analysis[J].Shandong Chemical Industry,2018,47(6):86-88