摘要
目的:建立UPLC法测定香砂养胃丸中木香烃内酯和去氢木香内酯的含量,以控制制剂中君药木香的质量。方法:采用ACQUITY UPLC BEH C_(18)(50 mm×2. 1 mm,1. 7μm)色谱柱,以乙腈-0. 1%磷酸为流动相,流速为0. 2 ml·min~(-1),测定波长为225 nm,柱温为30℃。结果:木香烃内脂、去氢木香内脂分别在1. 62~402. 98μg·ml~(-1)(r=0. 999 9)和1. 66~828. 80μg·ml~(-1)(r=0. 999 9)范围内线性关系良好,精密度、稳定性、重复性RSD均低于2. 0%,平均加样回收率分别为96. 65%(RSD=2. 05%)、92. 93%(RSD=2. 55%)。结论:该方法准确、可靠,可用于香砂养胃丸中木香烃内酯及去氢木香内酯的含量测定。
Objective: To establish a UPLC method to determine the contents of costunolide and dehydrocostuslactone in Xiangsha Yangwei pills for the quality control of radix aucklandiae,the main herb in the formula. Methods: An ACQUITY UPLC C_(18)( 50 mm ×2. 1 mm,1. 7 μm) chromatographic column was applied with acetonitrile and 0. 1% phosphoric acid as the mobile phase. The flow rate was 0. 2 ml·min~(-1),the detection wavelength was set at 225 nm,and the column temperature was 30 ℃. Results: The linear relationships of the two components were very good within the range of 1. 62-402. 98 μg·ml~(-1)( r = 0. 999 9) for costunolide and 1. 66-828. 80 μg·ml~(-1)( r = 0. 999 9) for dehydrocostulactone. The RSDs of precision,stability and repeatability were all below 2. 0%. The recovery was 96. 65%( RSD = 2. 05%) and 92. 93%( RSD = 2. 55%),respectively. Conclusion: The method is accurate and reliable,which can be used for the determination of costunolide and dehyrocostuslactone in Xiangsha Yangwei pills.
引文
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