云南猕猴桃细菌性溃疡病病原菌鉴定
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摘要
为明确云南省发生的猕猴桃溃疡病种类,通过田间发病症状、菌落形态、致病性测定、Biolog分析,16S rDNA序列分析比较,对疑似猕猴桃溃疡病病原菌进行鉴定。结果表明:病原菌接种可引起烟草过敏反应,同时在猕猴桃叶片表面形成带有黄色叶晕的不规则褐色斑点,将菌株测序序列与现有的丁香假单胞菌菌株的16S rDNA序列构建进化树,结果显示病原菌与丁香假单胞猕猴桃致病变种聚在同一分支上。以上表明云南省猕猴桃溃疡病病原菌为丁香假单胞猕猴桃致病变种(Pseudomonas syringae pv. actinidiae)。这是云南省首次报道由Pseudomonas syringae pv. actinidiae引起的猕猴桃细菌性溃疡病。
In order to clarify the pathogen causing kiwifruit bacterial canker in Yunnan Province,pathogen field symptoms study,colony morphology,pathogenicity assay,biolog analysis,16 S rDNA sequence analysis were conducted. The results showed that the pathogen caused tobacco allergic reactions,the symptoms on kiwi leaves were reddish brown spots surrounded by yellow haloes. The pathogen was clustered on the same branch as the Pseudomonas syringae pv. actinidiae in the phylogenetic tree constructed based on 16 S rDNA sequences of P. syringae strains. All the results revealed that the pathogen of kiwifruit bacterial canker belonged to P. syringae pv. actinidiae.This is the first report of P. syringae pv.actinidiae as a pathogen of kiwifruit bacterial canker in Yunnan Province.
In order to clarify the pathogen causing kiwifruit bacterial canker in Yunnan Province,pathogen field symptoms study,colony morphology,pathogenicity assay,biolog analysis,16 S rDNA sequence analysis were conducted. The results showed that the pathogen caused tobacco allergic reactions,the symptoms on kiwi leaves were reddish brown spots surrounded by yellow haloes. The pathogen was clustered on the same branch as the Pseudomonas syringae pv. actinidiae in the phylogenetic tree constructed based on 16 S rDNA sequences of P. syringae strains. All the results revealed that the pathogen of kiwifruit bacterial canker belonged to P. syringae pv. actinidiae.This is the first report of P. syringae pv.actinidiae as a pathogen of kiwifruit bacterial canker in Yunnan Province.
引文
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[10]雷庆,叶华智,余中树.猕猴桃溃疡病菌噬菌体的初步研究.安徽农业科学,2007,35(19):5995-5996.
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[12]赵利娜,胡家勇,叶振风,等.猕猴桃溃疡病病原菌的分子鉴定和致病力测定.华中农业大学学报,2012,31(5):604-608.
[13]张慧琴,毛雪琴,肖金平,等.猕猴桃溃疡病病原菌分子鉴定与抗性材料初选.核农学报,2014,28(7):1181-1187.
[2]Balestra G M,Mazzaglia A,Quattrucci A,et al.Curren status of bacterial canker spread on kiwifruit in Italy.Australasian Plant Disease Notes,2009,4(1):34-36.
[3]Dreo T,Pirc M,Ravnikar M,et al.First report of Pseudomonas syringae pv.actinidiae,the causal agent of bacterial canker of kiwifruit in Slovenia.Plant Disease,2011,95(95):1311.
[4]Balestra G M,Renzi M,Mazzaglia A.First report of bacterial canker of Actinidia deliciosa caused by Pseudomonas syringae pv.actinidiae in Portugal.New Disease Reports,2010,22(11):2510-2513.
[5]方炎祖,朱晓湘,王宇道.湖南猕猴桃病害调查研究初报.四川果树科技,1990(1):28-29.
[6]杨霞,陈陆,王川庆.16S r RNA基因序列分析技术在细菌分类中应用的研究进展.西北农林科技大学学报(自然科学版),2008,36(2):55-60.
[7]樊芳冰.四川猕猴桃溃疡病菌的分离鉴定及其粗毒素的研究[D].成都:四川农业大学,2012.
[8]Dan C O.Pseudomonas Canker of Kiwifruit.Plant Disease,1983,67(11):1283-1284.
[9]Takikawa Y,Serizawa S,Ichikawa T,et al.Pseudomonas syringae pv.actinidiae pv.nov.:the causal bacterium of canker of kiwifruit in Japan.Annals of the Phytopathological Society of Japan,1989,55(4):437-444.
[10]雷庆,叶华智,余中树.猕猴桃溃疡病菌噬菌体的初步研究.安徽农业科学,2007,35(19):5995-5996.
[11]申哲,黄丽丽,康振生.陕西关中地区猕猴桃溃疡病调查初报.西北农业学报,2009,18(1):191-193.
[12]赵利娜,胡家勇,叶振风,等.猕猴桃溃疡病病原菌的分子鉴定和致病力测定.华中农业大学学报,2012,31(5):604-608.
[13]张慧琴,毛雪琴,肖金平,等.猕猴桃溃疡病病原菌分子鉴定与抗性材料初选.核农学报,2014,28(7):1181-1187.