标准化兔下肢缺血模型的建立
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摘要
目的:探讨建立标准化兔下肢缺血模型的方法,为在缺血模型上进行的实验提供一个稳定的实验平台。方法:将12只新西兰大白兔随机分成2组,每组6只,实验组于左髂总动脉下段水平(髂内动脉与髂外动脉分支处),对照组于左股动脉起始部水平(腹股沟韧带处)结扎,在数字减影血管造影(DSA)下评估缺血模型建立后的下肢供血情况,术后第3天与第10天测股内侧体表温度并进行下肢大体观察。结果:实验组DSA下未发现明显可见的侧支动脉供血,对照组下肢造影则显示仍有部分髂内动脉侧支到达下肢供血,差异有统计学意义(P<0.05)。结论:于髂总动脉下段水平结扎后无明显可见的侧支血管到达下肢供血,可建立基本相同的兔下肢缺血模型。
Objective:To explore the standard method of lower limbs ischemia model establishment in rabbits to provide a consistent condition for the subsequent study on the basis of ischemia. Methods:Twelve New Zealand rabbits were divided into 2 groups(Group I and Group F)according to a random number table with 6 rabbits in each. The rabbits were ligated at the lower segment of the left common iliac artery(cross of internal and external iliac artery)in the group I,or at the start level of left femoral artery(inguinal ligament)in the group F,respectively,followed by DSA to observe the lower limbs blood supply,and measurement of vastus medial shell temperature at the 3 rd and 10 th day of ligation. Results:No blood supply of branch artery was observed in group I whereas there were still branches from the external iliac artery to the lower limb(P < 0.05). Conclusion:Ligation at the lower segment of the common iliac artery can promise to block the blood supply for the lower limb,which can be used to establish a consistent lower limb ischemia model.
Objective:To explore the standard method of lower limbs ischemia model establishment in rabbits to provide a consistent condition for the subsequent study on the basis of ischemia. Methods:Twelve New Zealand rabbits were divided into 2 groups(Group I and Group F)according to a random number table with 6 rabbits in each. The rabbits were ligated at the lower segment of the left common iliac artery(cross of internal and external iliac artery)in the group I,or at the start level of left femoral artery(inguinal ligament)in the group F,respectively,followed by DSA to observe the lower limbs blood supply,and measurement of vastus medial shell temperature at the 3 rd and 10 th day of ligation. Results:No blood supply of branch artery was observed in group I whereas there were still branches from the external iliac artery to the lower limb(P < 0.05). Conclusion:Ligation at the lower segment of the common iliac artery can promise to block the blood supply for the lower limb,which can be used to establish a consistent lower limb ischemia model.
引文
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[2]Fowkes FG,Rudan D,Rudan I,et al.Comparison of global estimates of prevalence and risk factors for peripheral artery disease in 2000 and 2010:a systematic review and analysis[J].Lancet,2013,382(9901):1329-1340
[3]Lu D,Chen B,Liang Z,et al.Comparison of bone marrow mesenchymal stem cells with bone marrow-derived mononuclear cells for treatment of diabetic critical limb ischemia and foot ulcer:a double-blind,randomized,controlled trial[J].Diabetes Res Clin Pract,2011,92(1):26-36
[4]Okita K,Ichisaka T,Yamanaka S,et al.Generation of germline-competent induced pluripotent stem cells[J].Nature,2007,448(7151):313-317
[5]张凯辉,徐宝山,杨强,等.骨髓间充质干细胞在多种疾病治疗中的应用进展[J].中国组织工程研究,2017,21(21):3400-3406
[6]Herring M,Gardner A,Glover J.A single-staged technique for seeding vascular grafts with autogenous endothelium[J].Surgery,1978,84(4):498-504
[7]Ding MC,Fang LI,Wang B,et al.Percutaneous endovascular angioplasty for the treatment of arteriosclerosis obliterans of the lower extremities showing no outflow tract visualization:a preliminary exploration[J].J Intervent Radiol,2015,5(5):383-387
[8]Del GC,Ifergan G,Goudot G,et al.Evaluation of a new model of hind limb ischemia in rabbit[J].J Vasc Surg,2017,pii:S0741-5214(17)32122-5.doi:10.1016/j.jvs.2017.07.140[.Epub ahead of print]
[9]Chen T,Chen D,Li F,et al.Netrin-1 with stem cells promote angiogenesis in limb ischemic rats[J].J Surg Res,2014,192(2):664-649
[10]Baltgalvis KA,White K,Li W,et al.Exercise performance and peripheral vascular insufficiency improve with AMPK activation in high-fat diet-fed mice[J].Am J Physiol Heart Circ Physiol,2014,306(8):H1128-1145
[11]Gao W,LI J.Femoral artery occlusion increases muscle pressor reflex and expression of hypoxia-inducible factor-1ɑin sensory neurons[J].J Cardiovasc Dis,2013,1(2):34-40
[12]Shin CI,Kim HC,Song YS,et al.Rat model of hindlimb ischemia induced via embolization with polyvinyl alcohol and N-butyl cyanoacrylate[J].Korean J Radiol,2013,14(6):923-930