大强度运动对大鼠外周血T淋巴细胞亚群和免疫球蛋白的影响
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摘要
目的探讨大强度运动恢复期外周血CK、BUN、免疫球蛋白、T淋巴细胞亚群的动态变化。方法大鼠40只,分为安静组、运动后即刻组、运动后12 h组、运动后24 h组,每组10只。安静组不运动,运动组进行9周大强度训练,安静组、运动即刻组末次训练后腹动脉取血,测定CK、BUN、 IgG、IgA、IgM、 CD~+_3、CD~+_4、CD~+_8、CD~+_4/CD~+_8。运动后12 h组、运动后24 h组分别在运动后12 h、运动后24 h,按同样方法取血和检测相同指标。结果与安静组相比,运动后即刻组CK、BUN升高,CD~+_3、CD~+_4、CD~+_4/CD~+_8、IgG、IgA、IgM降低,运动后12 h组CK升高,CD~+_3、CD~+_4、CD~+_4/CD~+_8、IgA降低,运动后24 h组CK降低;与运动后即刻组相比,运动后12 h组CK、CD~+_3、CD~+_4、CD~+_4/CD~+_8、TgA、TgM、TgG升高、BUN降低,运动后24 h组BUN降低,CD~+_3、CD~+_4、CD~+_4/CD~+_8、TgA、TgM、TgG升高;与运动后12 h组相比,运动24 h组CK降低,CD~+_3、CD~+_4、CD~+_4/CD~+_8、TgA升高。结论大强度运动恢复期间,CK于12 h达到高点后慢慢降低;BUN逐渐降低,24 h后基本恢复正常水平;CD~+_3、CD~+_4、CD~+_4/CD~+_8逐渐上升,24 h后基本恢复正常水平;TgA、TgM、TgG逐渐上升,TgM、TgG 12 h后基本恢复正常水平,TgA 24 h后基本恢复正常水平。
Objective To explore the dynamic characteristics of CK,BUN,immunoglobulin and T lymphocyte subsets in the peripheral blood.Methods 40 rats in total were divided into sedentary group(n=10),immediate group after exercise(n=10),12-hour group after exercise(n=10) and 24-hour group after exercise(n=10).Except for the sedentary group,the rats of other groups were carried out the high-intensity exercise in 9-week periods and taken abdominal vein blood to detect CK,BUN,IgG,IgA,IgM,CD~+_3,CD~+_4,CD~+_8 and CD~+_4/CD~+_8.12-hour group after exercise was detected after 12 hours and the 24-hour group after exercise was detected after 24 hours.The samples were taken and detected with the same methods.Results Comparing with the sedentary group,CK and BUN in immediate group after exercise increased,CD~+_3,CD~+_4,CD~+_4/CD~+_8,IgG,IgA and IgM in immediate group after exercise decreased,CK in 12-hour group after exercise increased,CD~+_3,CD~+_4,CD~+_4/CD~+_8 and IgA in 12-hour group after exercise decreased,and CK in 24-hour group after exercise decreased.Comparing with immediate group after exercise,CK,CD~+_3,CD~+_4,CD~+_4/CD~+_8,IgA,IgM and IgG in 12-hour group after exercise increased,BUN in 12-hour group after exercise decreased,BUN in 24-hour group after exercise decreased,and CD~+_3,CD~+_4,CD~+_4/CD~+_8,IgA,IgM and IgG in 24-hour group after exercise increased.Comparing with 12-hour group after exercise,CK in the 24-hour group after exercise decreased,and CD~+_3,CD~+_4,CD~+_4/CD~+_8 and IgA in the 24-hour group after exercise increased.Conclusion During the recovery period,CK gradually decreases after reaching the peak after 12 hours,BUN gradually decreases and returns to normal level after 24 hours,CD~+_3,CD~+_4 and CD~+_4/CD~+_8 increases and returns to normal level after 24 hours,IgM and IgG increases and returns to normal level after 12 hours,IgA increased and returned to normal level after 24 hours,respectively.
Objective To explore the dynamic characteristics of CK,BUN,immunoglobulin and T lymphocyte subsets in the peripheral blood.Methods 40 rats in total were divided into sedentary group(n=10),immediate group after exercise(n=10),12-hour group after exercise(n=10) and 24-hour group after exercise(n=10).Except for the sedentary group,the rats of other groups were carried out the high-intensity exercise in 9-week periods and taken abdominal vein blood to detect CK,BUN,IgG,IgA,IgM,CD~+_3,CD~+_4,CD~+_8 and CD~+_4/CD~+_8.12-hour group after exercise was detected after 12 hours and the 24-hour group after exercise was detected after 24 hours.The samples were taken and detected with the same methods.Results Comparing with the sedentary group,CK and BUN in immediate group after exercise increased,CD~+_3,CD~+_4,CD~+_4/CD~+_8,IgG,IgA and IgM in immediate group after exercise decreased,CK in 12-hour group after exercise increased,CD~+_3,CD~+_4,CD~+_4/CD~+_8 and IgA in 12-hour group after exercise decreased,and CK in 24-hour group after exercise decreased.Comparing with immediate group after exercise,CK,CD~+_3,CD~+_4,CD~+_4/CD~+_8,IgA,IgM and IgG in 12-hour group after exercise increased,BUN in 12-hour group after exercise decreased,BUN in 24-hour group after exercise decreased,and CD~+_3,CD~+_4,CD~+_4/CD~+_8,IgA,IgM and IgG in 24-hour group after exercise increased.Comparing with 12-hour group after exercise,CK in the 24-hour group after exercise decreased,and CD~+_3,CD~+_4,CD~+_4/CD~+_8 and IgA in the 24-hour group after exercise increased.Conclusion During the recovery period,CK gradually decreases after reaching the peak after 12 hours,BUN gradually decreases and returns to normal level after 24 hours,CD~+_3,CD~+_4 and CD~+_4/CD~+_8 increases and returns to normal level after 24 hours,IgM and IgG increases and returns to normal level after 12 hours,IgA increased and returned to normal level after 24 hours,respectively.
引文
[1] 李省天,漆正堂,丁树哲.急性运动对血清游离mtDNA以及先天免疫信号通路的影响[J].中国体育科技,2018,54(1):123-128.
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[10] 刘江,李毅钧.排球多元组合训练的生理生化监控及实施研究——以四川省男子排球队为例[J].广州体育学院学报,2016,36(1):102-105.
[11] 王志锋,高可丽,王东旭.运动应激对新疆少数民族大学生部分生理生化指标的影响[J].中国学校卫生,2016,37(1):11-15.
[12] 李国安,刘毅伟.古典式摔跤运动员夏训训期监测研究[J].武汉体育学院学报,2015,49(4):87-90.
[13] 李旭武.体育舞蹈锻炼对大学生血清免疫球蛋白及T淋巴细胞亚群的影响[J].体育学刊,2014,21(4):135-139.
[14] 聂健,王冀邯.运动对大鼠脾脏T淋巴细胞增殖及T细胞亚群的影响研究[J].中国免疫学杂志,2015,31(4):477-479.
[15] 高明,吴瑛,盛泽田,等.针灸预处理对摔跤运动员大负荷训练阶段NK细胞和T淋巴细胞亚群的影响[J].上海体育学院学报,2014,38(3):67-72.
[16] 卢咏梅,张宏.电针对大强度运动应激大鼠细胞免疫的影响[J].针刺研究,2012,37(2):136-139.
[17] 王晓琴,刘坚,袁际学,等.烟盒舞运动对中年女性血清免疫球蛋白和血脂的影响[J].运动,2017(18):155-156.
[18] 周彩华,王献升,莫伟彬,等.益生菌对运动大鼠免疫球蛋白、IL-6、TNF-α含量及HSP70表达的影响[J].现代预防医学,2018,45(10):1835-1838.
[2] 蔡国梁,郭霞,汪宇峰,等.不同负荷游泳运动对大鼠部分免疫指标的影响[J].哈尔滨体育学院学报,2017,35(2):1-5,11.
[3] 周宁,杨建全,冯锁民.急性递增负荷运动前后红细胞免疫功能与T淋巴细胞及其亚群的变化研究[J].中西医结合心脑血管病杂志,2017,15(5):602-603.
[4] Bedford TG,Tipton CM,Wilson NC,et al.Maximum oxygen consumption of rats and its changes with various experimental procedures[J].Journal of Applied Physiology,1979,47(6):1278-1283.
[5] 龚勋.不同运动强度对大学生免疫球蛋白、T淋巴细胞亚群水平的影响[J].黑龙江八一农垦大学学报,2016,28(4):143-146,154.
[6] 王茹,陈佩杰.运动诱导的T_H1/T_H2淋巴细胞亚群失衡机制研究进展[J].中国运动医学杂志,2009(1):112-117.
[7] 王成美,曾红兵.高强度运动诱发肌肉损伤对血糖调节、肌酸激酶、IL-6、TNF-α水平的影响[J].基因组学与应用生物学,2018,37(3):970-975.
[8] 孙洪亮.运动营养补剂对健美操运动员机体乳酸脱氢酶和肌酸激酶的影响研究[J].内蒙古师范大学学报(自然科学汉文版),2017,46(4):548-550.
[9] 陈万,田诗彬,吴春燕,等.运动疲劳恢复期不同时相大鼠丙二醛含量、超氧化物歧化酶活性及相关指标动态变化特征[J].中国康复医学杂志,2013,28(11):1001-1005,1014.
[10] 刘江,李毅钧.排球多元组合训练的生理生化监控及实施研究——以四川省男子排球队为例[J].广州体育学院学报,2016,36(1):102-105.
[11] 王志锋,高可丽,王东旭.运动应激对新疆少数民族大学生部分生理生化指标的影响[J].中国学校卫生,2016,37(1):11-15.
[12] 李国安,刘毅伟.古典式摔跤运动员夏训训期监测研究[J].武汉体育学院学报,2015,49(4):87-90.
[13] 李旭武.体育舞蹈锻炼对大学生血清免疫球蛋白及T淋巴细胞亚群的影响[J].体育学刊,2014,21(4):135-139.
[14] 聂健,王冀邯.运动对大鼠脾脏T淋巴细胞增殖及T细胞亚群的影响研究[J].中国免疫学杂志,2015,31(4):477-479.
[15] 高明,吴瑛,盛泽田,等.针灸预处理对摔跤运动员大负荷训练阶段NK细胞和T淋巴细胞亚群的影响[J].上海体育学院学报,2014,38(3):67-72.
[16] 卢咏梅,张宏.电针对大强度运动应激大鼠细胞免疫的影响[J].针刺研究,2012,37(2):136-139.
[17] 王晓琴,刘坚,袁际学,等.烟盒舞运动对中年女性血清免疫球蛋白和血脂的影响[J].运动,2017(18):155-156.
[18] 周彩华,王献升,莫伟彬,等.益生菌对运动大鼠免疫球蛋白、IL-6、TNF-α含量及HSP70表达的影响[J].现代预防医学,2018,45(10):1835-1838.