高酸值薏米糠油脱酸技术及其活性研究
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摘要
为降低薏米糠油酸值,提高其品质,采用响应面法优化了薏米糠油的碱炼脱酸工艺条件,并研究了精炼薏米糠油的活性。结果表明:薏米糠油碱炼脱酸最佳工艺条件为超量碱0.21%、碱炼时间33 min、碱液质量分数26.58%、碱炼温度58℃,在最佳工艺条件下薏米糠油酸值(KOH)从45.9 mg/g降到0.59 mg/g;精炼薏米糠油在质量浓度0.75~12 mg/m L范围内对酪氨酸酶的抑制率表现出良好的剂量依赖关系,IC_(50)为2 mg/m L;同时精炼薏米糠油对DPPH自由基的清除能力IC_(50)为6.36 mg/m L,表明其具有一定的美白、抗氧化功效。精炼薏米糠油是一种高品质、高生物活性的天然植物油,有望开发为功能性油脂产品。
In order to improve the quality and reduce the acid value of adlay bran oil,the alkali refining deacidification conditions of adlay bran oil were optimized by response surface methodology,and the activity of refined adlay bran oil was studied. The results showed that the optimal alkali refining deacidification conditions of adlay bran oil were obtained as follows: excessive alkali 0. 21%,alkali refining time 33 min,mass concentration of alkali solution 26. 58%,and alkali refining temperature 58 ℃. Under these conditions,the acid value of adlay bran oil reduced from 45. 9 mg KOH/g to 0. 59 mg KOH/g. Besides,the refined adlay bran oil had dosage-dependant relationship with inhibitory rate of tyrosinase in the mass concentration of 0. 75-12 mg/mL,and the IC_(50) was 2 mg/mL. The IC_(50) of refined adlay bran oil against DPPH radical was 6. 36 mg/mL. It turned out that adlay bran oil had certain whitening effect and antioxidant activity. In a word,refined adlay bran oil was a kind of natural vegetable oil with high quality and biological activity,which might be developed as functional oil in the future.
In order to improve the quality and reduce the acid value of adlay bran oil,the alkali refining deacidification conditions of adlay bran oil were optimized by response surface methodology,and the activity of refined adlay bran oil was studied. The results showed that the optimal alkali refining deacidification conditions of adlay bran oil were obtained as follows: excessive alkali 0. 21%,alkali refining time 33 min,mass concentration of alkali solution 26. 58%,and alkali refining temperature 58 ℃. Under these conditions,the acid value of adlay bran oil reduced from 45. 9 mg KOH/g to 0. 59 mg KOH/g. Besides,the refined adlay bran oil had dosage-dependant relationship with inhibitory rate of tyrosinase in the mass concentration of 0. 75-12 mg/mL,and the IC_(50) was 2 mg/mL. The IC_(50) of refined adlay bran oil against DPPH radical was 6. 36 mg/mL. It turned out that adlay bran oil had certain whitening effect and antioxidant activity. In a word,refined adlay bran oil was a kind of natural vegetable oil with high quality and biological activity,which might be developed as functional oil in the future.
引文
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[15]林莉,秦礼康,刘荣林,等.响应面法优化水酶法提取薏米糠油工艺研究[J].中国油脂,2015,40(4):1-5.
[16]吴云静,张勋,宋国辉,等.冷榨芝麻油酸法脱胶工艺[J].食品工业科技,2014,35(17):206-209.
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[19]李加兴,余娇,黄诚,等.猕猴桃籽油的体外抗氧化活性[J].食品科学,2012,33(23):51-54.
[20]刘玉兰,陈园顺,刘璐,等.碱炼脱酸工艺条件对高酸值米糠油碱炼效果的影响[J].粮食与食品工业,2014,21(3):7-11.
[2]ARORA R K.Job's-tears(Coix lachrymal-jobi)—a minor food and fodder crop from north eastern India[J].Econ Bot,1977,31(3):358-366.
[3]WU T T,CHARLES A L,HUANG T C.Determination of the contents of the main biochemical compounds of adlay(Coxi lachrymal-jobi)[J].Food Chem,2007,104(4):1509-1515.
[4]陆雅丽,王明力,闫岩.薏苡仁综合开发利用[J].中国食物与营养,2013,19(4):64-66.
[5]章洁琼,朱怡.贵州薏苡产业发展的现状及对策[J].贵州农业科学,2015,43(4):217-219.
[6]翁长江.薏苡副产品对兔及猪生长性能的影响[J].饲料博览,2013,38(10):37-39.
[7]华正根,王金亮,陈先娟,等.薏苡仁糠的综合利用[J].农业机械,2013,10(10):43-45.
[8]CHEN H J,HSU H Y,CHIANG W C.Allergic immuneregulatory effects of adlay bran on an OVA-immunized mice allergic model[J].Food Chem Toxicol,2012,50(10):3808-3813.
[9]CHEN H J,CHUNG C P,CHIANG W C,et al.Anti-inflammatory effects and chemical study of a flavonoid-enriched fraction from adlay bran[J].Food Chem,2011,126(4):1741-1748.
[10]HUANG D W,WW C H,SHIH C K,et al.Application of the solvent extraction technique to investigation of the anti-inflammatory activity of adlay bran[J].Food Chem,2014,145(15):445-453.
[11]LEE M Y,TSAI S H,KUO Y H,et al.Anti-tumor and anti-inflammatory activity of the methanol extracts from adlay bran[J].Food Sci Biotechnol,2008,17(6):1265-1271.
[12]LEE M Y,LIN H Y,CHENG F W,et al.Isolation and characterization of new lactam compounds that inhibit lung and colon cancer cells from adlay(Coix lachryma-jobi L.var.ma-yuen Stapf)bran[J].Food Chem Toxicol,2008,46(6):1933-1939.
[13]严航,唐婷,干丽,等.薏苡仁提取物对酪氨酸酶抑制作用[J].中成药,2013,35(4):696-699.
[14]蔡莹,汪岳刚,吴金鸿,等.超临界CO2流体提取薏仁米糠油及其脂肪酸成分分析[J].食品与药品,2012(9):309-312.
[15]林莉,秦礼康,刘荣林,等.响应面法优化水酶法提取薏米糠油工艺研究[J].中国油脂,2015,40(4):1-5.
[16]吴云静,张勋,宋国辉,等.冷榨芝麻油酸法脱胶工艺[J].食品工业科技,2014,35(17):206-209.
[17]郝志萍.小米糠油精炼及糠蜡制取工艺的研究[D].太原:山西大学,2015.
[18]赵能,肖丰坤,施蕊,等.滇牡丹籽油与凤丹牡丹籽油品质及活性对比研究[J].中国油脂,2016,41(6):92-95.
[19]李加兴,余娇,黄诚,等.猕猴桃籽油的体外抗氧化活性[J].食品科学,2012,33(23):51-54.
[20]刘玉兰,陈园顺,刘璐,等.碱炼脱酸工艺条件对高酸值米糠油碱炼效果的影响[J].粮食与食品工业,2014,21(3):7-11.