芽变突变体彩叶杨光合特性及叶绿体超微结构的研究
|
摘要
为探究彩叶杨芽变突变体中红杨和全红杨与野生型绿叶杨L2025生长差异的原因,本研究以芽变突变体彩叶杨中红杨和全红杨,以及野生型绿叶杨L2025为试验材料,对其叶绿体超微结构和光合特性进行研究。结果表明,中红杨和全红杨叶绿体基粒片层少,叶绿素合成受阻,其受阻位点可能位于CoprogenⅢ和ProtoⅨ之间,光合色素含量降低,光合作用减弱,净光速率仅为正常绿叶杨L2025的62%～73%。本研究从理论上证实了生产上全红杨、中红杨扦插生长慢的现象,为彩叶杨的推广繁殖提供了一定的参考依据。
In order to investigate the mechanism of growth differences between bud mutant of Zhonghong poplar and Quanhong poplar varieties and wild poplar,we used Zhonghong poplar( Populus deltoides Zhonghong,ZHP) originating from the bud sports of L2025 poplar and Quanhong poplar( Populus deltoids Quanhong,QHP) originating from the bud sports of ZHP and the green-leaf wild type L2025 to compare their chloroplast ultrastructure and photosynthetic characteristics. The results showed that the chloroplast basal laminas in the mesophyll cell of ZHP and QHP leaves were significantly decreased and chlorophyll biosynthesis was blocked. The blacked site might be located between Coprogen Ⅲand Proto Ⅸ,which further caused a significant decrease in photosynthetic pigments in the ZHP and QHP when compared to the wide type. Therefore,the photosynthesis capacities of the mutants were largely reduced,and the net photosynthetic rates were only 62% ～ 73% of the wide type poplar. This study theoretically confirmed the low growth of ZHP and QHP cuttings in production,which provides a certain reference for the promotion and production of colorleaved poplar varieties.
In order to investigate the mechanism of growth differences between bud mutant of Zhonghong poplar and Quanhong poplar varieties and wild poplar,we used Zhonghong poplar( Populus deltoides Zhonghong,ZHP) originating from the bud sports of L2025 poplar and Quanhong poplar( Populus deltoids Quanhong,QHP) originating from the bud sports of ZHP and the green-leaf wild type L2025 to compare their chloroplast ultrastructure and photosynthetic characteristics. The results showed that the chloroplast basal laminas in the mesophyll cell of ZHP and QHP leaves were significantly decreased and chlorophyll biosynthesis was blocked. The blacked site might be located between Coprogen Ⅲand Proto Ⅸ,which further caused a significant decrease in photosynthetic pigments in the ZHP and QHP when compared to the wide type. Therefore,the photosynthesis capacities of the mutants were largely reduced,and the net photosynthetic rates were only 62% ～ 73% of the wide type poplar. This study theoretically confirmed the low growth of ZHP and QHP cuttings in production,which provides a certain reference for the promotion and production of colorleaved poplar varieties.
引文
[1] Li Y H,Wang B H,Dai Z Y,Li A H,Liu G Q,Zuo S M,ZhangH X,Pan X B. Morphological structure and genetic mapping of newleaf-color mutant gene in rice(Oryza sativa)[J]. Rice Science,2012,19(2):79-85
[2] Córdoba J,Molina-cano J L,Martínez-Carrasco R,Morcuende R,Pérez P. Functional and transcriptional characterization of a barleymutant with impaired photosynthesis[J]. Plant Science anInternational Journal of Experimental Plant Biology,2016,244:19-30
[3] Zhang C H,Yang H Y,Wang X M,Li W L,Wu WL. Identificationof a thymidine kinase(RUTK1)homolog differentially expressed inblackberry(Rubus L.)prickles[J]. Pakistan Journal of Botany,2016,48(6):2513-2520
[4] Gelderen C JⅤ,Gelderen D MⅤ. Maples for Gardens:A ColorEncyclopedia[M]. Portlant:Timber Press,1999:1-294
[5]朱延林,程相军.杨树新品种‘中红杨’[J].林业科学,2008,44(10):173-174
[6]武立权,沈圣泉,王荣富,舒庆尧.水稻黄叶突变体光合特性的日变化[J].核农学报,2007,21(5):425-429
[7]马春雷,姚明哲,王新超,金基强,马建强,陈亮.茶树叶绿素合成相关基因克隆及在白叶1号不同白化阶段的表达分析[J].作物学报,2015,41(2):240-250
[8]栾海业,臧慧,沈会权,张英虎,乔海龙,陶红,申玉香,陈和.大麦白化颖壳突变体的转录组学分析[J].核农学报,2017,31(12):2332-2339
[9] Walker A R,Lee E,Robinson S P. Two new grape cultivars,budsports of Cabernet Sauvignon bearing pale-coloured berries,are theresult of deletion of two regulatory genes of the berry colour locus[J]. Plant Molecular Biology,2006,62(4/5):623-635
[10] Zhou X S,Shen S Q,Wu D X,Sun J W,Shu Q Y. Introduction ofa xantha mutation for testing and increasing varietal purity in hybridrice[J]. Field Crops Research,2006,96(1):71-79
[11]曹桦,李涵,苗振,付国宝,杨春梅,吴丽芳,赵培飞,单芹丽,阮继伟,汪国鲜,王继华,李绅崇.铁皮石斛叶色突变体初步研究[J].核农学报,2017,31(3):461-471
[12]孙丽娟,蔣学皎,谢锋香,吴殿星,祝水金,陈进红.玉米自交系低植酸突变体的生物学与光合特性研究[J].核农学报,2010,24(4):668-674
[13]王彩霞,田韦韦,田敏,欧阳彤,汪凤珍.文心兰黄化突变体的初步研究[J].核农学报,2013,27(12):1845-1852
[14]张宪政.植物叶绿素含量测定-丙酮乙醇混合液法[J].辽宁农业科学,1986(3):26-27
[15] Ubi B E,Honda C,Bessho H,Kondo S,Wada M,Kobayashi S,Moriguchi T. Expression analysis of anthocyanin biosynthetic genesin apple skin:effect of UV-B and temperature[J]. Plant Science,2006,170(3):571-578
[16]王凌健,倪迪安,叶叙丰,夏镇澳,刘剑荣.植物叶片中8-氨基乙酰丙酸的测定[J].植物生理学通讯,1997,35(6):439-441
[17] Bogorad L. Porphydn synthesis[C]//Colowick S P,Kapha N O.Methods in Enzymology. New York:Academic Process,1962:885-891
[18] Rebeiz C A,Mattheis J L,Smith B B,Rebeiz C C,Dayton D F.Chloroplast biosynthesis and accumulation of protochlorophyll byisolated etioplasts and developing chloroplasts[J]. Archives ofBiochemistry and Biophysics,1975,167(1):351
[19]万雪琴,张帆,夏新莉,尹伟伦.镉处理对杨树光合作用及叶绿素荧光参数的影响[J].林业科学,2008,44(6):73-78
[20]刘仁林,虞功清,邹伟民,杜天真.植物透射电镜样品制备技术的改进[J].江西林业科技,2008(1):41-43
[21] Ono M,Iwashina T. Quantitative flavonoid variation accompanied bychange of flower colors in EDEG worthia chrysantha,Pittosporumtobira and Wisteria floribunda[J]. Natural ProductCommunications,2015,10(3):413-416
[22] Jornet-Somoza J,Alberdi-Rodriguez J,Milne B F,Andrade X,Marques M A,Nogueira F,Oliveira M J,Stewart J J,Rubio A.Insights into colour-tuning of chlorophyll optical response in greenplants[J]. Physical Chemistry Chemical Physics,2015,17(40):26599-26606
[23] Liu Q,Xu J,Liu Y Z,Zhao X L,Deng X X,Guo L L,Gu J Q. Anovel bud mutation that confers abnormal patterns of lycopeneaccumulation in sweet orange fruit(Citrus sinensis L. Osbeck)[J].Journal of Experimental Botany,2007,58(15/16):4161-4171
[24] Chang Q S,Chen S M,Chen Y,Deng Y M,Chen F D,Zhang F,Wang S W. Anatomical and physiological differences anddifferentially expressed genes between the green and yellow leaftissue in a variegated chrysanthemum variety[J]. MolecularBiotechnology,2013,54(2):393-411
[25] Hanke G,Mulo P. Plant type ferredoxins and ferredoxin-dependentmetabolism[J]. Plant Cell Environ,2013,36(6):1071-1084
[26] Yuxia Y,Xingxu C,Bin X,Yang Y,Chen X,Xu B,Li Y,Ma Y,Wang G. Phenotype and transcriptome analysis reveals chloroplastdevelopment and pigment biosynthesis together influenced the leafcolor formation in mutants of Anthurium andraeanum‘Sonate’[J].Fronties in Plant Science,2015,6:139
[27]王彩霞,田韦韦,田敏,欧阳彤,汪凤珍.文心兰黄化突变体的初步研究[J].核农学报,2013,27(12):1845-1852
[28]吕明,刘海衡,毛虎德,赵惠贤,胡胜武.芥菜型油菜黄化突变体叶片叶绿素合成代谢变化[J].西北植物学报,2010,30(11):2177-2183
[29]刘彩云.普通烟草中的白肋型烟草叶色性状遗传及其质体色素差异性研究[D].北京:中国农业科学院,201l
[30]刘中华,计玮玮,鲁聪,徐式近,谈建中.彩叶富贵竹叶绿素生物合成代谢的研究[J].安徽农业科学,2009,37(19):8963-8964
[31]宗学凤,罗力,吕典华,王三根,何光华.水稻叶片全生育期黄化突变体ylg3叶绿素合成特性研究[J].西南大学学报(自然科学版),2013,35(11):21-26
[32] Zhang K M,Wang X M,Cui J X,Ogweno J O,Shi K,Zhou Y H,Yu J Q. Characteristics of gas exchange and chlorophyll fluorescencein red and green leaves of Begonia semperflorens[J]. BiologiaPlantarum,2011,55(2):361-364
[33]徐培洲,李云,袁澍,张红宇,彭海,林宏辉,汪旭东,吴先军.叶绿素缺乏水稻突变体中光系统蛋白和叶绿素合成特性的研究[J].中国农业科学,2006,39(7):1299-1305
[34]常青山,张利霞,陈煜,陈素梅,刘兆磊,房伟民,陈发棣.菊花黄绿叶突变体的光合与类囊体膜光谱[J].林业科学,2013,49(2):72-78
[35] Zhang F,Zhao J,Wan X,Luo X,Li W,Sun L,Chen Q. Erratumto:from green to red:large-scale transcriptome comparison of a budsport in poplar(Populus deltoides)[J]. Acta PhysiologiaePlantarum,2017,39(4):108
[2] Córdoba J,Molina-cano J L,Martínez-Carrasco R,Morcuende R,Pérez P. Functional and transcriptional characterization of a barleymutant with impaired photosynthesis[J]. Plant Science anInternational Journal of Experimental Plant Biology,2016,244:19-30
[3] Zhang C H,Yang H Y,Wang X M,Li W L,Wu WL. Identificationof a thymidine kinase(RUTK1)homolog differentially expressed inblackberry(Rubus L.)prickles[J]. Pakistan Journal of Botany,2016,48(6):2513-2520
[4] Gelderen C JⅤ,Gelderen D MⅤ. Maples for Gardens:A ColorEncyclopedia[M]. Portlant:Timber Press,1999:1-294
[5]朱延林,程相军.杨树新品种‘中红杨’[J].林业科学,2008,44(10):173-174
[6]武立权,沈圣泉,王荣富,舒庆尧.水稻黄叶突变体光合特性的日变化[J].核农学报,2007,21(5):425-429
[7]马春雷,姚明哲,王新超,金基强,马建强,陈亮.茶树叶绿素合成相关基因克隆及在白叶1号不同白化阶段的表达分析[J].作物学报,2015,41(2):240-250
[8]栾海业,臧慧,沈会权,张英虎,乔海龙,陶红,申玉香,陈和.大麦白化颖壳突变体的转录组学分析[J].核农学报,2017,31(12):2332-2339
[9] Walker A R,Lee E,Robinson S P. Two new grape cultivars,budsports of Cabernet Sauvignon bearing pale-coloured berries,are theresult of deletion of two regulatory genes of the berry colour locus[J]. Plant Molecular Biology,2006,62(4/5):623-635
[10] Zhou X S,Shen S Q,Wu D X,Sun J W,Shu Q Y. Introduction ofa xantha mutation for testing and increasing varietal purity in hybridrice[J]. Field Crops Research,2006,96(1):71-79
[11]曹桦,李涵,苗振,付国宝,杨春梅,吴丽芳,赵培飞,单芹丽,阮继伟,汪国鲜,王继华,李绅崇.铁皮石斛叶色突变体初步研究[J].核农学报,2017,31(3):461-471
[12]孙丽娟,蔣学皎,谢锋香,吴殿星,祝水金,陈进红.玉米自交系低植酸突变体的生物学与光合特性研究[J].核农学报,2010,24(4):668-674
[13]王彩霞,田韦韦,田敏,欧阳彤,汪凤珍.文心兰黄化突变体的初步研究[J].核农学报,2013,27(12):1845-1852
[14]张宪政.植物叶绿素含量测定-丙酮乙醇混合液法[J].辽宁农业科学,1986(3):26-27
[15] Ubi B E,Honda C,Bessho H,Kondo S,Wada M,Kobayashi S,Moriguchi T. Expression analysis of anthocyanin biosynthetic genesin apple skin:effect of UV-B and temperature[J]. Plant Science,2006,170(3):571-578
[16]王凌健,倪迪安,叶叙丰,夏镇澳,刘剑荣.植物叶片中8-氨基乙酰丙酸的测定[J].植物生理学通讯,1997,35(6):439-441
[17] Bogorad L. Porphydn synthesis[C]//Colowick S P,Kapha N O.Methods in Enzymology. New York:Academic Process,1962:885-891
[18] Rebeiz C A,Mattheis J L,Smith B B,Rebeiz C C,Dayton D F.Chloroplast biosynthesis and accumulation of protochlorophyll byisolated etioplasts and developing chloroplasts[J]. Archives ofBiochemistry and Biophysics,1975,167(1):351
[19]万雪琴,张帆,夏新莉,尹伟伦.镉处理对杨树光合作用及叶绿素荧光参数的影响[J].林业科学,2008,44(6):73-78
[20]刘仁林,虞功清,邹伟民,杜天真.植物透射电镜样品制备技术的改进[J].江西林业科技,2008(1):41-43
[21] Ono M,Iwashina T. Quantitative flavonoid variation accompanied bychange of flower colors in EDEG worthia chrysantha,Pittosporumtobira and Wisteria floribunda[J]. Natural ProductCommunications,2015,10(3):413-416
[22] Jornet-Somoza J,Alberdi-Rodriguez J,Milne B F,Andrade X,Marques M A,Nogueira F,Oliveira M J,Stewart J J,Rubio A.Insights into colour-tuning of chlorophyll optical response in greenplants[J]. Physical Chemistry Chemical Physics,2015,17(40):26599-26606
[23] Liu Q,Xu J,Liu Y Z,Zhao X L,Deng X X,Guo L L,Gu J Q. Anovel bud mutation that confers abnormal patterns of lycopeneaccumulation in sweet orange fruit(Citrus sinensis L. Osbeck)[J].Journal of Experimental Botany,2007,58(15/16):4161-4171
[24] Chang Q S,Chen S M,Chen Y,Deng Y M,Chen F D,Zhang F,Wang S W. Anatomical and physiological differences anddifferentially expressed genes between the green and yellow leaftissue in a variegated chrysanthemum variety[J]. MolecularBiotechnology,2013,54(2):393-411
[25] Hanke G,Mulo P. Plant type ferredoxins and ferredoxin-dependentmetabolism[J]. Plant Cell Environ,2013,36(6):1071-1084
[26] Yuxia Y,Xingxu C,Bin X,Yang Y,Chen X,Xu B,Li Y,Ma Y,Wang G. Phenotype and transcriptome analysis reveals chloroplastdevelopment and pigment biosynthesis together influenced the leafcolor formation in mutants of Anthurium andraeanum‘Sonate’[J].Fronties in Plant Science,2015,6:139
[27]王彩霞,田韦韦,田敏,欧阳彤,汪凤珍.文心兰黄化突变体的初步研究[J].核农学报,2013,27(12):1845-1852
[28]吕明,刘海衡,毛虎德,赵惠贤,胡胜武.芥菜型油菜黄化突变体叶片叶绿素合成代谢变化[J].西北植物学报,2010,30(11):2177-2183
[29]刘彩云.普通烟草中的白肋型烟草叶色性状遗传及其质体色素差异性研究[D].北京:中国农业科学院,201l
[30]刘中华,计玮玮,鲁聪,徐式近,谈建中.彩叶富贵竹叶绿素生物合成代谢的研究[J].安徽农业科学,2009,37(19):8963-8964
[31]宗学凤,罗力,吕典华,王三根,何光华.水稻叶片全生育期黄化突变体ylg3叶绿素合成特性研究[J].西南大学学报(自然科学版),2013,35(11):21-26
[32] Zhang K M,Wang X M,Cui J X,Ogweno J O,Shi K,Zhou Y H,Yu J Q. Characteristics of gas exchange and chlorophyll fluorescencein red and green leaves of Begonia semperflorens[J]. BiologiaPlantarum,2011,55(2):361-364
[33]徐培洲,李云,袁澍,张红宇,彭海,林宏辉,汪旭东,吴先军.叶绿素缺乏水稻突变体中光系统蛋白和叶绿素合成特性的研究[J].中国农业科学,2006,39(7):1299-1305
[34]常青山,张利霞,陈煜,陈素梅,刘兆磊,房伟民,陈发棣.菊花黄绿叶突变体的光合与类囊体膜光谱[J].林业科学,2013,49(2):72-78
[35] Zhang F,Zhao J,Wan X,Luo X,Li W,Sun L,Chen Q. Erratumto:from green to red:large-scale transcriptome comparison of a budsport in poplar(Populus deltoides)[J]. Acta PhysiologiaePlantarum,2017,39(4):108