心可宁胶囊中非法添加三七茎叶的检测
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摘要
目的建立心可宁胶囊中非法添加三七茎叶的检测方法。方法采用薄层色谱法(TLC)和高效液相色谱法(HPLC)检测心可宁胶囊中三七茎叶投料。结果若样品中检出人参皂苷Rb3,则为三七茎叶投料。结果表明:心可宁胶囊存在以三七茎叶非法投料的现象。结论该方法简便可行,准确度高,不存在假阳性干扰。
Objective To establish a method for the determination of illegally added Panax notoginseng leaves in Xinkening Capsules.Methods TLC and HPLC was used to check Panax notoginseng leaves in Xinkening Capsules.The two methods validated each other and made the results more accurate.Results Illegally added Panax notoginseng leaves were determination by detection of ginsenoside Rb3.The results confirmed that that there existed adulteration in Xinkening Capsules.Conclusion The method is simple and accurate for the identification of Panax notoginseng leaves in Xinkening Capsules,and excludes the possibility of the false positive.
Objective To establish a method for the determination of illegally added Panax notoginseng leaves in Xinkening Capsules.Methods TLC and HPLC was used to check Panax notoginseng leaves in Xinkening Capsules.The two methods validated each other and made the results more accurate.Results Illegally added Panax notoginseng leaves were determination by detection of ginsenoside Rb3.The results confirmed that that there existed adulteration in Xinkening Capsules.Conclusion The method is simple and accurate for the identification of Panax notoginseng leaves in Xinkening Capsules,and excludes the possibility of the false positive.
引文
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[2]《卫生部药品标准》中药成方制剂第九册,心可宁胶囊[S].1994:48-49.
[3]中国科学院植物研究所.中国高等植物图鉴[M].北京:科学出版社,1972:1045.
[4]马金双,于海英.中国五加科药用植物资源[J].中草药,1989,20(11):43.
[5]国家药典委员会.中国药典2015年版一部[S].北京:中国医药科技出版社,2015:11-12.
[6]刘翀,韩金玉,常贺英,等.三七茎叶提取物中人参皂苷R b 3的分离及指纹图谱[J].天津大学学报,2006,39(10):1151-1156.
[7]国家药典委员会.中国药典2015年版四部[S].北京:中国医药科技出版社,2015:57-59.
[8]周家明,崔秀明,曾鸿超,等.三七茎叶的综合开发利用[J].现代中药研究与实践,2009,23(3):32-33.
[9]魏均娴,唐宝书,王菊芳,等.三七叶皂苷成分的研究[J].华西药学杂志,1986,1(1):7.
[10]黄捷,覃红萍.复方丹参片中非法添加的三七茎叶的检测[J].中国药业,2011,20(14):32-33.
[11]费毅琴,杨萍,肖凌,等.HPLC法快速筛查中成药中三七/人参茎叶代替根和根茎[J].中成药,2015,37(8):1730-1733.
[12]林伟鑫,姚曦,李勇,等.三七总皂苷提取、大孔树脂纯化工艺研究[J].食品与药品,2015,17(3):156-161.
[13]何耀慧,林荣锋,陈剑平,等.人参、三七在复方芪术调经散中的高效液相色谱法鉴别[J].广州中医药大学学报,2009,26(5):468-470.