从三七茎叶皂苷中分离纯化人参皂苷C-Mx1和Rb3
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摘要
利用两步硅胶柱层析法,从三七茎叶皂苷中分离纯化人参皂苷Rb3和C-Mx1单体。30g三七茎叶皂苷,经分离得到纯度为50%~60%的粗品C-Mx1 4.66g和Rb3 8.58g;将C-Mx1和Rb3粗品皂苷进行二次硅胶柱分离,得到纯度为95%的C-Mx1单体0.92g和Rb3单体3.1g。以核磁共振法证明了分离产物为C-Mx1和Rb3皂苷。
The ginsenoside C-Mx1 and Rb3 were isolated and purified from notoginseng leaf ginsenosides.30 g notoginseng leaf ginsenosides were isolated,from which 50%-60% crude C-Mx1 of 4.66 g and Rb1 of 8.58 g were obtained.95% pure C-Mx1 of 0.92 g and Rb3 of 3.1 g were obtained from 4.66 g crude C-Mx1 and 8.58 g crude Rb3.The separation products were C-Mx1 and Rb3 saponins determined by ~(13) C NMR.
The ginsenoside C-Mx1 and Rb3 were isolated and purified from notoginseng leaf ginsenosides.30 g notoginseng leaf ginsenosides were isolated,from which 50%-60% crude C-Mx1 of 4.66 g and Rb1 of 8.58 g were obtained.95% pure C-Mx1 of 0.92 g and Rb3 of 3.1 g were obtained from 4.66 g crude C-Mx1 and 8.58 g crude Rb3.The separation products were C-Mx1 and Rb3 saponins determined by ~(13) C NMR.
引文
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[2]张均田.人参冠百草—人参化学、生物学活性和药代动力学研究进展[M].北京:化学工业出版社,2008.
[3]王凤,周亚滨.人参皂苷Rb3的药理研究[J].中医药学报,2012,40(1):93-95.
[4]刘东平,杨军,丁丹.三七及其有效成分对血液系统的药理活性研究概况[J].中医药信息,2012(4):172-173.
[5]饶高雄,张维明,王梅,等.人参皂苷Rb3对照品的制备研究[J].云南中医学院学报,2006,29(2):8-10.
[6]韩金玉,刘翀,王华,等.正相液相制备色谱分离纯化三七叶皂苷中人参皂苷单体Rb3[J].高校化学工程学报,2005,19(2):192-196.
[7]刘翀,韩金玉,常贺英,等.三七茎叶提取物中人参皂苷Rb3的分离及指纹图谱[J].天津大学学报,2006,39(10):1151-1156.
[8]黄凤,向飞军,伍杰雄.三七叶皂苷成分及其单体分离研究进展[J].中药材,2009,32(6):999-1004.
[9]姜彬慧,王承志,韩颖,等.三七叶中微量活性皂苷的分离与鉴定[J].中药材,2004,27(7):489-491.
[10]AZIMOVA S S.Natural Compounds—Triterpene Glycosides[M].New York:Springer Science Business Media,2013.
[11]HE K J,LIU Y,YANG Y,et al.A dammarane glycoside derived from ginsenoside Rb3[J].Chemical and Pharmaceutical Bulletin,2005,53(2):177-179.