摘要
利用两步硅胶柱层析法,从三七茎叶皂苷中分离纯化人参皂苷Rb3和C-Mx1单体。30g三七茎叶皂苷,经分离得到纯度为50%~60%的粗品C-Mx1 4.66g和Rb3 8.58g;将C-Mx1和Rb3粗品皂苷进行二次硅胶柱分离,得到纯度为95%的C-Mx1单体0.92g和Rb3单体3.1g。以核磁共振法证明了分离产物为C-Mx1和Rb3皂苷。
The ginsenoside C-Mx1 and Rb3 were isolated and purified from notoginseng leaf ginsenosides.30 g notoginseng leaf ginsenosides were isolated,from which 50%-60% crude C-Mx1 of 4.66 g and Rb1 of 8.58 g were obtained.95% pure C-Mx1 of 0.92 g and Rb3 of 3.1 g were obtained from 4.66 g crude C-Mx1 and 8.58 g crude Rb3.The separation products were C-Mx1 and Rb3 saponins determined by ~(13) C NMR.
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