三七茎叶总皂苷对心肌缺血再灌注损伤保护作用研究
|
摘要
目的:研究三七茎叶总皂苷(PNSSL)对缺血再灌注损伤大鼠心肌的保护作用,为PNSSL的开发利用提供基础。方法:将SD大鼠随机分为6组,即假手术组、模型组40,80,160 mg·kg-1组、地奥心血康组,连续给药七天,末次给药后通过结扎冠状动脉左前降支的方法建立大鼠缺血再灌注损伤模型。观察缺血再灌注大鼠II导联心电图ST段的变化情况,采用2,3,5-氯化三苯基四氮唑(TTC)染色法检测心肌梗死面积,测定大鼠血清中乳酸脱氢酶(LDH)、肌酸激酶(CK)、谷草转氨酶(AST)、谷丙转氨酶(ALT)活性以及心肌组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性和丙二醛(MDA)含量,通过苏木精-伊红(HE)染色法观察缺血再灌心肌的病理形态。结果:与模型组相比,PNSSL各剂量组对于大鼠心电图ST段升高均具有降低作用,能够减少心肌梗死范围,其中PNSSL80,160 mg·kg-1组降低显著(P<0.05);PNSSL160 mg·kg-1剂量能够明显降低血清中心肌酶活性(P<0.05),PNSSL80 mg·kg-1显著升高心肌组织中SOD和GSH-Px的活性(P<0.05)。病理染色中,PNSSL组心肌组织间质水肿明显减轻,纤维排列整齐,心肌组织损伤降低。结论:三七茎叶总皂苷能够改善由缺血再灌注引起的心肌组织损伤,改善心电图变化,提高细胞膜稳定性,清除氧自由基,减少炎性浸润,具有心肌保护作用。
Objective To explore the protective effect of saponins in stems and leaves of Panax notoginseng onmyocardial ischemic reperfusion injury in rats. Methods The rats were divided into six groups(the sham operation group,model group, DAXXK group, PNSSL 40, 80, 160 mg·kg-1 group) and continuous oral administration for 7 days. The ratmodel of myocardial ischemic reperfusion injury(MIRI) was developed by ligation of the left anterior descending coronaryartery the electrocardiogram(ECG),myocardial infarct size, staining with hematoxylin and eosin were observed. Theactivities of LDH、CK、AST、ALT in serum and SOD、GSH-Px、MDA in cardiac muscle tissue were detected by kit.Results Saponins in stems and leaves of Panax notoginseng in each dose(40, 80, 160 mg · kg-1) group significantlyreduced the myocardial infarct size and improved the ECG on ST segment, the myocardial damage was obviously reducedfrom the pathological section. Stems and leaves of Panax notoginseng in a dose of 160 mg · kg-1 lowered the serumactivities of LDH、CK and AST, contents of SOD and GSH-Px in a dose of 80 mg·kg-1 significantly lowered. Conclusions The results indicates that PNSSL have the effect against myocardial ischemic reperfusion injury and that the mechanismof pharmacological action related to the improvement of ECG changes, the stability of cell membrane, eliminate oxygenfree radicals and the reducing of inflammatory infiltration.
Objective To explore the protective effect of saponins in stems and leaves of Panax notoginseng onmyocardial ischemic reperfusion injury in rats. Methods The rats were divided into six groups(the sham operation group,model group, DAXXK group, PNSSL 40, 80, 160 mg·kg-1 group) and continuous oral administration for 7 days. The ratmodel of myocardial ischemic reperfusion injury(MIRI) was developed by ligation of the left anterior descending coronaryartery the electrocardiogram(ECG),myocardial infarct size, staining with hematoxylin and eosin were observed. Theactivities of LDH、CK、AST、ALT in serum and SOD、GSH-Px、MDA in cardiac muscle tissue were detected by kit.Results Saponins in stems and leaves of Panax notoginseng in each dose(40, 80, 160 mg · kg-1) group significantlyreduced the myocardial infarct size and improved the ECG on ST segment, the myocardial damage was obviously reducedfrom the pathological section. Stems and leaves of Panax notoginseng in a dose of 160 mg · kg-1 lowered the serumactivities of LDH、CK and AST, contents of SOD and GSH-Px in a dose of 80 mg·kg-1 significantly lowered. Conclusions The results indicates that PNSSL have the effect against myocardial ischemic reperfusion injury and that the mechanismof pharmacological action related to the improvement of ECG changes, the stability of cell membrane, eliminate oxygenfree radicals and the reducing of inflammatory infiltration.
引文
1 Hausenloy D J,Yellon D M.Myocardial ischemia-reperfusion injury:a neglected therapeutic target.The Journal of clinical investigation,2013,123(1):92-100.
2 Lejay A,Fei F,John R,et al.Ischemia reperfusion injury,ischemic conditioning and diabetes mellitus.Journal of Molecular&Cellular Cardiology,2016,91:11.
3 胡飞,戎亦骊,朱科燕,等.冠心宁片对大鼠心肌缺血再灌注损伤的保护作用研究.中国比较医学杂志,2017,27(5):76-82.
4 赖晓峰,马康华,秦春常,等.IGF-1后处理与缺血后处理对缺血再灌注心肌保护作用的对比研究.解放军医学杂志,2011,36(8):808-812.
5 许波华,许立.中药抗心肌缺血作用机制的研究进展.中国实验方剂学杂志,2011,17(15):265.
6 李冀,王秀珍,李在斯,等.中药治疗心肌缺血再灌注损伤的研究进展.中医药学报,2015(2):107-109.
7 占海思,潘涛.红景天在心肌缺血再灌注损伤中保护机制的研究进展.中国实验方剂学杂志,2016(8):231-234.
8 Yu Z,Wang S,Zhang X,et al.Pterostilbene protects against myocardial ischemia/reperfusion injury via suppressing oxidative/nitrative stress and inflammatory response..International Immunopharmacology,2017,43:7-15.
9 周家明,崔秀明,曾鸿超,等.三七茎叶的综合开发利用.现代中药研究与实践,2009,23(3):32.
10 黄胜英,谢世荣,黄彩云,等.三七叶皂苷抗心律失常作用的实验研究.大连大学学报,2001,22(6):82.
11 向胜群,向明利.三七叶神安片的临床应用.现代医药卫生,2005,21(24):3453.
12 任小宇,孙桂波,张强,等.三七茎叶皂苷对大鼠离体心脏缺血/再灌注损伤的保护作用.中国药理学通报,2012,28(1):92-96.
13 付建华,李欣志,尚晓泓,等.三七茎叶皂苷对麻醉犬急性心肌缺血的保护作用.中国中药杂志,2006,31(1):62-65.
14 温剑艺,谭宁,杨大浩.大鼠心肌缺血-再灌注模型的心电图变化.岭南心血管病杂志,2011,17(6):503-506.
15 徐明亮,王丽丽,李琳,等.白鲜皮水提物对大鼠心肌缺血再灌注损伤的保护作用.中国实验动物学报,2013,21(1):47-52.
16 Hausenloy D J,Kharbanda R,Rahbek S M,et al.Effect of remote ischaemic conditioning on,clinical outcomes in patients presenting with an,ST-segment elevation myocardial infarction,undergoing primary percutaneous coronary,intervention..European Heart Journal,2015,36(29):1846.
17 刘永国,任澎.心肌缺血/再灌注损伤的机制研究进展.医学综述,2010,16(21):3267-3269.
18 邓海英,赖为国.心肌缺血再灌注损伤的机制研究进展.中国医药指南,2013,11(1):63-64.
19 Yellon D M,Hausenloy D J.Myocardial reperfusion injury.New England Journal of Medicine,2007,357(11):1121-1135.
20 Rajadurai M,Stanely Mainzen Prince P.Preventive effect of naringin on lipid peroxides and antioxidants in isoproterenol-induced cardiotoxicity in Wistar rats:Biochemical and histopathological evidences.Toxicology,2006,228(2-3):259-68.
21 陈虹,朱鲲鹏,张真,等.地奥心血康对心肌缺血再灌注损伤的保护作用及机制研究.中草药,2010,41(12):2018-2023.
22 Chen C,Lu W,Wu G,et al.Cardioprotective effects of combined therapy with diltiazem and superoxide dismutase on myocardial ischemia-reperfusion injury in rats.Life Sciences,2017,183:50-59.
23 陈冰心,任澎,段明军,等.大鼠心肌缺血再灌注模型构建的改良.中国比较医学杂志,2010,20(5):24-28.
24 董六一,邵旭,江勤,等.牡荆素对大鼠实验性心肌缺血损伤的保护作用及其机制.中草药,2011,42(7):1378-1383.
2 Lejay A,Fei F,John R,et al.Ischemia reperfusion injury,ischemic conditioning and diabetes mellitus.Journal of Molecular&Cellular Cardiology,2016,91:11.
3 胡飞,戎亦骊,朱科燕,等.冠心宁片对大鼠心肌缺血再灌注损伤的保护作用研究.中国比较医学杂志,2017,27(5):76-82.
4 赖晓峰,马康华,秦春常,等.IGF-1后处理与缺血后处理对缺血再灌注心肌保护作用的对比研究.解放军医学杂志,2011,36(8):808-812.
5 许波华,许立.中药抗心肌缺血作用机制的研究进展.中国实验方剂学杂志,2011,17(15):265.
6 李冀,王秀珍,李在斯,等.中药治疗心肌缺血再灌注损伤的研究进展.中医药学报,2015(2):107-109.
7 占海思,潘涛.红景天在心肌缺血再灌注损伤中保护机制的研究进展.中国实验方剂学杂志,2016(8):231-234.
8 Yu Z,Wang S,Zhang X,et al.Pterostilbene protects against myocardial ischemia/reperfusion injury via suppressing oxidative/nitrative stress and inflammatory response..International Immunopharmacology,2017,43:7-15.
9 周家明,崔秀明,曾鸿超,等.三七茎叶的综合开发利用.现代中药研究与实践,2009,23(3):32.
10 黄胜英,谢世荣,黄彩云,等.三七叶皂苷抗心律失常作用的实验研究.大连大学学报,2001,22(6):82.
11 向胜群,向明利.三七叶神安片的临床应用.现代医药卫生,2005,21(24):3453.
12 任小宇,孙桂波,张强,等.三七茎叶皂苷对大鼠离体心脏缺血/再灌注损伤的保护作用.中国药理学通报,2012,28(1):92-96.
13 付建华,李欣志,尚晓泓,等.三七茎叶皂苷对麻醉犬急性心肌缺血的保护作用.中国中药杂志,2006,31(1):62-65.
14 温剑艺,谭宁,杨大浩.大鼠心肌缺血-再灌注模型的心电图变化.岭南心血管病杂志,2011,17(6):503-506.
15 徐明亮,王丽丽,李琳,等.白鲜皮水提物对大鼠心肌缺血再灌注损伤的保护作用.中国实验动物学报,2013,21(1):47-52.
16 Hausenloy D J,Kharbanda R,Rahbek S M,et al.Effect of remote ischaemic conditioning on,clinical outcomes in patients presenting with an,ST-segment elevation myocardial infarction,undergoing primary percutaneous coronary,intervention..European Heart Journal,2015,36(29):1846.
17 刘永国,任澎.心肌缺血/再灌注损伤的机制研究进展.医学综述,2010,16(21):3267-3269.
18 邓海英,赖为国.心肌缺血再灌注损伤的机制研究进展.中国医药指南,2013,11(1):63-64.
19 Yellon D M,Hausenloy D J.Myocardial reperfusion injury.New England Journal of Medicine,2007,357(11):1121-1135.
20 Rajadurai M,Stanely Mainzen Prince P.Preventive effect of naringin on lipid peroxides and antioxidants in isoproterenol-induced cardiotoxicity in Wistar rats:Biochemical and histopathological evidences.Toxicology,2006,228(2-3):259-68.
21 陈虹,朱鲲鹏,张真,等.地奥心血康对心肌缺血再灌注损伤的保护作用及机制研究.中草药,2010,41(12):2018-2023.
22 Chen C,Lu W,Wu G,et al.Cardioprotective effects of combined therapy with diltiazem and superoxide dismutase on myocardial ischemia-reperfusion injury in rats.Life Sciences,2017,183:50-59.
23 陈冰心,任澎,段明军,等.大鼠心肌缺血再灌注模型构建的改良.中国比较医学杂志,2010,20(5):24-28.
24 董六一,邵旭,江勤,等.牡荆素对大鼠实验性心肌缺血损伤的保护作用及其机制.中草药,2011,42(7):1378-1383.