紫草对心房颤动模型大鼠的影响
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摘要
目的:探讨紫草对房颤模型大鼠的心房颤动的发生及发展的作用及相关机制。方法:大鼠通过尾静脉连续给乙酰胆碱-氯化钙(Ach-CaCl_2)造模。第6天开始,紫草组予紫草汤药灌胃,胺碘酮组予胺碘酮溶液灌胃,空白组和模型组予生理盐水灌胃。于第14天进行心电图检查并记录房颤的诱发时间及持续时间,之后,超声心电图检查以记录左房面积(LA area)、射血分数(EF%)、左室舒张末径(LVDD)、左室收缩末径(LVDS),以评估紫草对模型大鼠的心脏重构和功能的影响。结果:1.紫草降低心房颤动大鼠的心房颤动诱发率并缩短了心房颤动的持续时间;2.紫草使心房颤动大鼠的心房重构改善,表现为左房面积和左室内径的减小,但对EF值无明显影响。
Objects: This study aimed to investigate whether Radix Arnebiae could improve the atrial fibrillation in rats. Method: The model of atrial fibrillation in rats was established by Ach-CaCl_2 solutions through tail vein injection. Since the 6-th day,,the Radix Arnebiae herb soup and Amiodarone solution were administered via gavage, separately. The vehicle group and model group were treated with 0.9% Naclsoulution. Twenty days later, electrocardiograph were used to record ECG, the AF duration time and the AF induced frequency After, the echocardiograms were used to examine the LA area、EF、LVDD and LVDS. Conclusion: Radix Arnebiae can reduce the morbidity and duration of atrial fibrillation in rats. Radix Arnebiae can also improve the LA area 、LVDD、LVDS in rats, except EF.
Objects: This study aimed to investigate whether Radix Arnebiae could improve the atrial fibrillation in rats. Method: The model of atrial fibrillation in rats was established by Ach-CaCl_2 solutions through tail vein injection. Since the 6-th day,,the Radix Arnebiae herb soup and Amiodarone solution were administered via gavage, separately. The vehicle group and model group were treated with 0.9% Naclsoulution. Twenty days later, electrocardiograph were used to record ECG, the AF duration time and the AF induced frequency After, the echocardiograms were used to examine the LA area、EF、LVDD and LVDS. Conclusion: Radix Arnebiae can reduce the morbidity and duration of atrial fibrillation in rats. Radix Arnebiae can also improve the LA area 、LVDD、LVDS in rats, except EF.
引文
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[2] WOLF P A,ABBOTT R D,KANNEL W B.Atrial fibrillation as an independent risk factor for stroke:the Framingham Study[J].Stroke,1991,22:983-988.
[3] LAUPACIS A,BOYSEN G,Connolly S,et al.Risk factors for stroke and efficacy of antithrombotic therapy in atrial fibrillation.Analysis of pooled data from five randomized controlled trials[J].Arch Intern Med,1994,154:1449-1457.
[4] BUNCH T J,WEISS J P,CRANDALL B G,et al.Atrial fibrillation is independently associated with senile,vascular,and Alzheimer’s dementia[J].Heart Rhythm,2010,7:433-437.
[5] SHINDO S,HOSOKAWA Y,HOSOKAWA I,et al.Shikonin Inhibits Inflammatory Cytokine Production in Human Periodontal Ligament Cells[J].Inflammation,2016,39(3):1124-1129.
[6] 张改平,杨建雄,朱玉安.紫草提取物的体外抗氧化活性研究[J].植物科学学报,2007,25(5):490-493.
[7] YANG J,WANG Z,CHEN D L.Shikonin ameliorates isoproterenol (ISO)-induced myocardial damage through suppressing fibrosis,inflammation,apoptosis and ER stress[J].Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie,2017,93:1343.
[8] WASAKIL Y,NISHIDA K,KATO T,et al.Atrial Fibrillation Pathophysiology Implications for Management[J].Circulation,2011,124(20):2264-2274.
[9] ENGELMANNN M D,SVENDSEN J H.Inflammation in the genesis and perpetuation of atrial fibrillation.[J].European Heart Journal,2005,26(20):2083-2092.
[10] WU G,CHENG M,HUANG H,et al.A Variant of IL6R Is Associated with the Recurrence of Atrial Fibrillation after Catheter Ablation in a Chinese Han Population[J].Plos One,2014,9(6):e99623.
[11] 陈春林,巩甜甜,汤依群,等.SD大鼠房颤模型的建立[J].实验动物科学,2009,26(3):1-4.