纳米硅以及纳米三氧化二铁对大鼠中枢神经行为的影响
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摘要
目的:研究探讨纳米硅(nano-Si)及纳米三氧化二铁(nano-Fe_2O_3)对大鼠中枢神经行为的影响。方法:40只Wistar大鼠随机分为对照组(C组)、nano-Si低剂量组(SL组)、nano-Si高剂量组(SH组)、nano-Fe_2O_3低剂量组(FL组)和nano-Fe_2O_3高剂量组(FH)组,每组8只。采用灌胃方式给药4周,实验组给予纳米颗粒物与生理盐水悬浊液,对照组给予相应剂量生理盐水。给药量为低剂量组25 mg/kg、高剂量组100 mg/kg。通过旷场实验和Morris水迷宫实验检测大鼠的行为学和学习记忆功能。结果:在旷场实验中纳米颗粒高剂量实验组(SH、FH组)大鼠穿行格数、直立次数均显著低于对照组(P<0.05)。水迷宫实验,高剂量实验组(SH、FH组)大鼠在定位航行试验中逃避潜伏期明显长于同时期对照组(P<0.05),而空间探索实验中目的象限游泳时间明显低于对照组(P<0.05)。结论:一定剂量的纳米颗粒物可对神经系统造成损伤,影响大鼠的神经行为。
Objective:To investigate the effects of nano-Si and nano-Fe_2O_3 on central nervous behavior in rats.Method:40 Wistar rats were randomly divided into four groups:control group(C group),low and high dose of nano-Si groups(SL,SH group),low and high dose of nano-Fe_2O_3 groups(FL,FH group),8 rats in each group.The rats were exposed to nanometer particles through intragastric administration at 25 and 100 mg/kg body weight every day for 4 weeks.After exposure,the ability of learning and memory of rats were tested by Morris water maze and open field test.Result:Compared with the C group,the number of crossing and rearing decreased significantly in SH and FH group(P<0.05).In the space exploration experiment of Morris water maze test,the escape latency of SH and FH group were longer than those of C group(P<0.05).The rats of SH and FH group spent less time in finding the target quadrant(P<0.05).Conclusion:Nano-Si and nano-Fe_2O_3 can cause damage in the nervous system of rats after exposure and affect on the nervous behavior.
Objective:To investigate the effects of nano-Si and nano-Fe_2O_3 on central nervous behavior in rats.Method:40 Wistar rats were randomly divided into four groups:control group(C group),low and high dose of nano-Si groups(SL,SH group),low and high dose of nano-Fe_2O_3 groups(FL,FH group),8 rats in each group.The rats were exposed to nanometer particles through intragastric administration at 25 and 100 mg/kg body weight every day for 4 weeks.After exposure,the ability of learning and memory of rats were tested by Morris water maze and open field test.Result:Compared with the C group,the number of crossing and rearing decreased significantly in SH and FH group(P<0.05).In the space exploration experiment of Morris water maze test,the escape latency of SH and FH group were longer than those of C group(P<0.05).The rats of SH and FH group spent less time in finding the target quadrant(P<0.05).Conclusion:Nano-Si and nano-Fe_2O_3 can cause damage in the nervous system of rats after exposure and affect on the nervous behavior.
引文
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[2]Wang C,Hu X,Gao Y,et al.Zn O nanoparticles treatment induces apoptosis by increasing intracellular ROS levels in LTEP-a-2 cells[J].Biomed Res Int,2015,2015(5617):423287.
[3]Kim Y R,Park S H,Lee J K,et al.Organization of research team for nano-associated safety assessment in effort to study nanotoxicology of zinc oxide and silica nanoparticles[J].Int JNanomedicine,2014,9(Suppl 2):3-10.
[4]Ma H,Williams P L,Diamond S A.Ecotoxicity of manufactured Zn O nanoparticles:a review[J].Environ Pollut,2013,172(1):76-85.
[5]Ye X,Lian Q,Eckenhoff M F,et al.Differential general anesthetic effects on microglial cytokine expres-sion[J].PLo S One,2013,8(1):e52887.
[6]Cao Y L,Zhang W,Ai Y Q,et al.Effect of propofol and ketamine anesthesia on cognitive function and immune function in young rats[J].Asian Pacific Journal of Tropical Medicine,2014(5):89-92.
[7]Hu R,Gong X,Duan Y,et al.Neurotoxicological effects and the impairment of spatial recognition memory in mice caused by exposure to Ti O2 nanoparticles[J].Biomaterials,2010,31(31):8043-8050.
[8]Medina C,Santos-Martinez M J,Radomski A.Nanoparticles:pharmacological and toxicological significance[J].Br J Pharmacol,2007,150(5):552-558.
[9]Wang J X,Zhou G Q,Chen C Y,et al.Acute toxicity and biodistribution of different sized titanium dioxide particles in mice after oral administration[J].Toxicol Lett,2007,168(2):176-185
[10]Wang H F,Wang J,Deng X Y,et al.Biodistribution of carbon single-wall carbon nanotubes in mice[J].J Nanosci Nanotechnol,2004,4(8):1019-1024.
[11]刘丹,汪涛,何开华.异丙酚和氯胺酮对大鼠认知及免疫功能影响的研究[J].暨南大学学报(自然科学与医学版),2016,37(1):55-59.
[12]陈文,梁向党,孙赓,等.应用6%纳米二氧化硅改性氰基丙烯酸酯抗菌胶的抗菌性研究[J].解放军医药杂志,2015,27(3):37-40.
[13]齐锋.有氧运动对应激大鼠行为及相关神经内分泌指标影响的实验研究[J].内蒙古师范大学学报(自然科学汉文版),2013,42(4):490-493.
[14]马栋栋,张娜,马强,等.纳米二氧化硅及氧化锌对大鼠旷场行为及认知功能的影响[J].中华行为医学与脑科学杂志,2012,21(12):1080-1081.
[15]赵振,苏铁柱.中等强度有氧运动大鼠环境应激的保护效应及其机制探讨[J].长治学院学报,2012,29(5):78-82.
[16]王前,段盛明.李盛龙,等.社会隔离对大鼠空间和非空间认知功能的影响[J].中毕行为医学与脑科学杂志,2011,20(9):793-795.
[17]林晓春,李云鹏,卞艳芳,等.大鼠旷场实验指标检测及参考值的探讨[J].毒理学杂志,2010(3):224-225.
[18]宋淑玲,王君明.MSG对大鼠学习记忆能力及旷场行为影响的实验研究[J].广州医药,2008,39(1):1-3.
[19]胡镜清,温泽淮,赖世隆.Morris水迷宫检测的记忆属性与方法学初探[J].广州中医药大学学报,2000,6(17):117-119.
[20]郭德玉,陈铁玉,李斌,等.不同年龄大鼠学习记忆能力及矿场行为比较[J].实验动物科学与管理,1998(1):19-23.