大飞扬草的三维荧光光谱研究
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摘要
通过荧光分光光度计测定了不同采集时间的大飞扬草整株、叶、茎、根水提物的三维荧光光谱图,得出了可以用于鉴定的共有峰位置。绘制了峰位置分布图,直观地展现了各个荧光物质存在的部位。结合文献,指出了部分荧光峰的归属,未归属的荧光峰可能是属于新成分。
Three-dimensional fluorescence spectrums of water extracts of whole plant,leaves,stems and roots of Euphorbia hirta Linn were measured using fluorescence spectrophotometer. Common peaks were given for identification. A graph of the positions of the fluorescence peaks clearly showed which part of the plants contain certains chemicals that could emit fluorescence. Some fluorescence peaks were assigned to chemicals according to references. The peaks can not be assigned may belong to components that have not been discovered.
Three-dimensional fluorescence spectrums of water extracts of whole plant,leaves,stems and roots of Euphorbia hirta Linn were measured using fluorescence spectrophotometer. Common peaks were given for identification. A graph of the positions of the fluorescence peaks clearly showed which part of the plants contain certains chemicals that could emit fluorescence. Some fluorescence peaks were assigned to chemicals according to references. The peaks can not be assigned may belong to components that have not been discovered.
引文
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[3]国家药典委员会.中华人民共和国药典(2015年版)(一部)[M].北京:中国医药科技出版社出版社,2015.
[4]宋龙,徐宏喜,杨莉,等.飞扬草的化学成分与药理活性研究概况[J].中药材,2012,35(6):1 003-1 009.
[5]武毅,曲玮,耿頔,等.大飞扬草地上部位的酚类和黄酮类化合物(英文)[J].中国天然药物,2012,10(1):40-42.
[6]王莉,李盈,杨梦莹,等.飞扬草的化学成分研究(Ⅱ)[J].中成药,2014,36(8):1 687-1 692.
[7]黄勇红,黄艳萍,陈任宏.飞扬草药材HPLC指纹图谱研究[J].中国实验方剂学杂志,2012,18(16):74-76.
[8] Wang L,Wu H L,Yin X L, et al. Simultaneous determi-nation of umbelliferone and scopoletin in Tibetan medicine Saussure a laniceps and traditional Chinese medicine Radix angelicae pubescentis using excitation-emission matrix fluorescence coupled with second-order calibration method[J].Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy,2017(1):104-110.
[9]王丽.二阶校正方法在中药与食品质量安全领域的基础应用研究[D].长沙:湖南大学,2017.
[10]杨春飞.中药成分滨蒿内酯、7-甲氧基香豆素的荧光性质与分析方法研究[D].石家庄:河北师范大学,2011.
[11]周晓霞.基于荧光分析法的丁香酚提取工艺[J].衡水学院学报,2011, 13(4):43-44.
[12]杨莉,刘德龙,魏永巨.三维荧光结合二阶校正法测定绞股蓝中槲皮素含量[J].分析测试学报,2014, 33(9):1 038-1 043,1 049.
[13]刘博.敏化荧光法测定中药成分山奈酚、没食子酸的研究[D].石家庄:河北师范大学,2011.
[14]董怡.光果甘草叶黄酮的分离纯化、活性研究及应用[D].广州:华南理工大学,2016.
[15]李强.黄酮类化合物与牛血清白蛋白的相互作用研究[D].南昌:南昌大学,2011.