肝癌组织上皮细胞转化序列2基因的表达及其临床意义
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摘要
目的探讨肝癌组织上皮细胞转化序列2(ECT2)基因的表达及其临床意义。方法从GEO数据库下载相关芯片数据,选取GPL3921平台包含的220例正常肝组织和215例肝癌组织及其附带临床信息进行分析。根据ECT2基因(219787_s_at)表达值的中位数(5. 54)将肝癌患者分为ECT2基因低表达组107例和ECT2基因高表达组108例,比较两组患者的临床特征以及预后。采用Cox模型分析肝癌患者预后的影响因素。结果肝癌组织中ECT2基因表达水平高于正常肝组织(P <0. 05);相比于ECT2基因低表达组,ECT2基因高表达组的甲胎蛋白(AFP)水平、原发肿瘤-区域淋巴结-远处转移(TNM)分期和肿瘤转移风险更高,肿瘤直径更大,预后更差(P <0. 05)。多因素Cox分析结果显示,AFP>300 ng/ml、TNM分期Ⅱ～Ⅲ期、高转移风险、ECT2基因高表达均是患者预后的独立危险因素(均P <0. 05)。结论 ECT2基因在肝癌组织中高表达,是肝癌患者预后的独立危险因素,ECT2基因高表达患者预后较差。
Objective To investigate the expression and clinical significance of epithelial cell transforming sequence 2( ECT2)gene in hepatocellular carcinoma tissues. Methods The related chip data were downloaded from the GEO database. Totally 220 cases of normal liver tissues and 215 cases of hepatocellular carcinoma tissues from GPL3921 platform as well as their clinical data were enrolled in the study. The patients with hepatocellular carcinoma were divided into low-expression ECT2 gene group( n = 107) and high-expression ECT2 gene group( n = 108) according to the median( 5. 54) expression level of ECT2 gene( 219787 _s _at). The clinical features and prognosis were compared between the two groups. The factors influencing the prognosis of patients with hepatocellular carcinoma were analyzed using the Cox model. Results The expression level of ECT2 gene in hepatocellular carcinoma tissues was higher than that in normal liver tissues( P < 0. 05); compared to the low-expression ECT2 gene group,the high-expression ECT2 gene group had higher alpha-fetoprotein( AFP) level,higher tumor-node-metastasis( TNM) stage,higher risk of tumor metastasis,greater tumor diameter and poorer prognosis( P < 0. 05). The results of multivariate Cox analysis showed that AFP > 300 ng/ml,TNM stage Ⅱ-Ⅲ,high risk of metastasis,and high expression of ECT2 gene were independent risk factors for prognosis( all P < 0. 05). Conclusion ECT2 gene is highly expressed in hepatocellular carcinoma tissues and is an independent risk factor for prognosis of patients with hepatocellular carcinoma. Patients with high expression of ECT2 gene have a poorer prognosis.
Objective To investigate the expression and clinical significance of epithelial cell transforming sequence 2( ECT2)gene in hepatocellular carcinoma tissues. Methods The related chip data were downloaded from the GEO database. Totally 220 cases of normal liver tissues and 215 cases of hepatocellular carcinoma tissues from GPL3921 platform as well as their clinical data were enrolled in the study. The patients with hepatocellular carcinoma were divided into low-expression ECT2 gene group( n = 107) and high-expression ECT2 gene group( n = 108) according to the median( 5. 54) expression level of ECT2 gene( 219787 _s _at). The clinical features and prognosis were compared between the two groups. The factors influencing the prognosis of patients with hepatocellular carcinoma were analyzed using the Cox model. Results The expression level of ECT2 gene in hepatocellular carcinoma tissues was higher than that in normal liver tissues( P < 0. 05); compared to the low-expression ECT2 gene group,the high-expression ECT2 gene group had higher alpha-fetoprotein( AFP) level,higher tumor-node-metastasis( TNM) stage,higher risk of tumor metastasis,greater tumor diameter and poorer prognosis( P < 0. 05). The results of multivariate Cox analysis showed that AFP > 300 ng/ml,TNM stage Ⅱ-Ⅲ,high risk of metastasis,and high expression of ECT2 gene were independent risk factors for prognosis( all P < 0. 05). Conclusion ECT2 gene is highly expressed in hepatocellular carcinoma tissues and is an independent risk factor for prognosis of patients with hepatocellular carcinoma. Patients with high expression of ECT2 gene have a poorer prognosis.
引文
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[10]董志珍,朱小东,李照,等. 2016年肝癌基础与临床研究进展[J].中华肝脏病杂志,2017,25(2):85-93.
[11] Jin Y,Yu Y,Shao Q,et al. Up-regulation of ECT2 is associated with poor prognosis in gastric cancer patients[J]. Int J Clin Exp Pathol,2014,7(12):8 724-8 731.
[12] Yen CC,Chen YJ,Pan CC,et al. Copy number changes of target genes in chromosome 3q25. 3-qter of esophageal squamous cell carcinoma:TP63 is amplified in early carcinogenesis but downregulated as disease progressed[J]. World J Gastroenterol,2005,11(9):1 267-1 272.
[13] Justilien V,Ali SA,Jamieson L,et al. Ect2-Dependent rRNA Synthesis Is Required for KRAS-TRP53-Driven Lung Adenocarcinoma[J]. Cancer Cell,2017,31(2):256-269.
[14] Chen CJ,Sung WW,Chen HC,et al. Early Assessment of Colorectal Cancer by Quantifying Circulating Tumor Cells in Peripheral Blood:ECT2 in Diagnosis of Colorectal Cancer[J]. Int J Mol Sci,2017,18(4). pii:E743.
[2] Bruix J,Gores GJ,Mazzaferro V. Hepatocellular carcinoma:clinical frontiers and perspectives[J]. Gut,2014,63(5):844-855.
[3] Dang H,Takai A,Forgues M,et al. Oncogenic Activation of the RNA Binding Protein NELFE and MYC Signaling in Hepatocellular Carcinoma[J]. Cancer Cell,2017,32(1):101-114,e8.
[4] Ye QH,Zhu WW,Zhang JB,et al. GOLM1 Modulates EGFR/RTK Cell-Surface Recycling to Drive Hepatocellular Carcinoma Metastasis[J]. Cancer Cell,2016,30(3):444-458.
[5] Matthews HK,Delabre U,Rohn JL,et al. Changes in Ect2localization couple actomyosin-dependent cell shape changes to mitotic progression[J]. Dev Cell,2012,23(2):371-383.
[6] Murata Y,Minami Y,Iwakawa R,et al. ECT2 amplification and overexpression as a new prognostic biomarker for early-stage lung adenocarcinoma[J]. Cancer Sci,2014,105(4):490-497.
[7] Luo Y,Qin SL,Mu YF,et al. Elevated expression of ECT2predicts unfavorable prognosis in patients with colorectal cancer[J]. Biomed Pharmacother,2015,73:135-139.
[8] Wang HB,Yan HC,Liu Y. Clinical significance of ECT2expression in tissue and serum of gastric cancer patients[J]. Clin Transl Oncol,2016,18(7):735-742.
[9]代智,樊嘉.肝癌转移复发的生物学研究进展[J].中华实验外科杂志,2012,29(10):1 883-1 885.
[10]董志珍,朱小东,李照,等. 2016年肝癌基础与临床研究进展[J].中华肝脏病杂志,2017,25(2):85-93.
[11] Jin Y,Yu Y,Shao Q,et al. Up-regulation of ECT2 is associated with poor prognosis in gastric cancer patients[J]. Int J Clin Exp Pathol,2014,7(12):8 724-8 731.
[12] Yen CC,Chen YJ,Pan CC,et al. Copy number changes of target genes in chromosome 3q25. 3-qter of esophageal squamous cell carcinoma:TP63 is amplified in early carcinogenesis but downregulated as disease progressed[J]. World J Gastroenterol,2005,11(9):1 267-1 272.
[13] Justilien V,Ali SA,Jamieson L,et al. Ect2-Dependent rRNA Synthesis Is Required for KRAS-TRP53-Driven Lung Adenocarcinoma[J]. Cancer Cell,2017,31(2):256-269.
[14] Chen CJ,Sung WW,Chen HC,et al. Early Assessment of Colorectal Cancer by Quantifying Circulating Tumor Cells in Peripheral Blood:ECT2 in Diagnosis of Colorectal Cancer[J]. Int J Mol Sci,2017,18(4). pii:E743.