胸腔积液细胞蜡块免疫组化检测对胸腔积液的价值分析
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摘要
目的分析胸腔积液细胞蜡块免疫组化检测对胸腔积液的价值。方法方便选取2018年2月—2019年2月该院诊断的96例胸腔积液病患,进行随机分组,46例病患进行单纯细胞涂片HE染色作为参照组,50例病患进行细胞蜡块免疫组化检测作为探究组,观察并对比诊断结果。结果不同方式诊断后对比两组诊断准确率,探究组98.00%显著高于参照组86.96%(χ~2=4.32,P<0.05);对比两组检测结果的阳性率,探究组阳性率100.00%显著高于参照组78.26%(χ~2=10.45,P<0.05);96例胸腔积液病患中,48例诊断为查见腺癌细胞,48例为查见反应性增生间皮细胞。从抗体表达来看,发现腺癌细胞的特异性抗体有CK7、CEA和TTF-1,反应性增生间皮细胞的特异性抗体有WT-1、Calretinin和CK5/6(P<0.05)。结论细胞蜡块免疫组化检测对胸腔积液的诊断准确率高,值得临床推广。
Objective To analyze the value of immunohistochemical detection of pleural effusion cells in pleural effusion.Methods A total of 96 patients with pleural effusion diagnosed in our hospital from February 20 to February 2019 were convenient enrolled in a randomized group. 46 patients underwent HE staining with a single cell smear as a reference group,and 50 patients underwent cell waxing block immunohistochemistry was used as an exploration group to observe and compare the diagnosis results. Results After diagnosis, the diagnostic accuracy of the two groups was compared. 98.00% of the exploration group was significantly higher than the reference group by 86.96%(χ~2=4.32, P<0.05). The positive rate of the two groups was 100.00%, higher than the reference group of 78.26%(χ~2=10.45, P<0.05); of the 96 patients with pleural effusion, 48 were diagnosed as adenocarcinoma cells, and 48 were found to be reactive hyperplastic mesothelial cells. From the expression of antibodies, specific antibodies against adenocarcinoma cells were found to be CK7, CEA and TTF-1, and specific antibodies to reactive proliferative mesothelial cells were WT-1, Calretinin and CK5/6(P<0.05). Conclusion The immunohistochemical detection of cell wax block has a high diagnostic accuracy for pleural effusion, which is worthy of clinical promotion.
Objective To analyze the value of immunohistochemical detection of pleural effusion cells in pleural effusion.Methods A total of 96 patients with pleural effusion diagnosed in our hospital from February 20 to February 2019 were convenient enrolled in a randomized group. 46 patients underwent HE staining with a single cell smear as a reference group,and 50 patients underwent cell waxing block immunohistochemistry was used as an exploration group to observe and compare the diagnosis results. Results After diagnosis, the diagnostic accuracy of the two groups was compared. 98.00% of the exploration group was significantly higher than the reference group by 86.96%(χ~2=4.32, P<0.05). The positive rate of the two groups was 100.00%, higher than the reference group of 78.26%(χ~2=10.45, P<0.05); of the 96 patients with pleural effusion, 48 were diagnosed as adenocarcinoma cells, and 48 were found to be reactive hyperplastic mesothelial cells. From the expression of antibodies, specific antibodies against adenocarcinoma cells were found to be CK7, CEA and TTF-1, and specific antibodies to reactive proliferative mesothelial cells were WT-1, Calretinin and CK5/6(P<0.05). Conclusion The immunohistochemical detection of cell wax block has a high diagnostic accuracy for pleural effusion, which is worthy of clinical promotion.
引文
[1]李清苗.胸腔积液细胞蜡块免疫组化检测对胸腔积液的价值分析[J/OL].中国医学工程,2019(1):107-110.
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[3]唐忠庆,郑赓唐.分析胸腔积液细胞蜡块免疫组化检测的病理诊断价值[J].中国农村卫生,2016(12):3.
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[5]谢刚,原庆会,柯琦,等.免疫组化在老年胸腔积液细胞蜡块病理诊断中的应用[J].四川医学,2015(7):935-937.
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[7]林静,李美琼,农林琳.胸腔积液细胞蜡块联合免疫组化在肺腺癌与间皮细胞增生鉴别诊断中的应用[J].现代医药卫生,2016(3):338-339.
[8]黄其文,姜汉国.胸腔积液细胞蜡块切片免疫组化染色技术鉴别诊断肺腺癌的临床研究[J].临床肺科杂志,2017,22(2):227-229.
[9]蒙秋萍,薛逢贵,罗志飞,等.胸腔积液细胞蜡块技术在分子病理检测上的应用价值[J].世界最新医学信息文摘,2017(45):228-229.
[10]黄晓萍.肺腺癌恶性胸腔积液细胞蜡块的诊断价值及表皮生长因子受体表达的临床意义[J].中国基层医药,201825(16):2049.
[11]钱红,钟鹏,赵娟霞,等.多种细胞学方法联合检查不明原因渗出性胸腔积液的诊断价值[J].中国实验诊断学,201822(3):11-12.
[2]余燕琪.胸腔积液病理诊断中应用细胞蜡块切片免疫组化染色检测的价值[J].医疗装备,2017,30(24):68-69.
[3]唐忠庆,郑赓唐.分析胸腔积液细胞蜡块免疫组化检测的病理诊断价值[J].中国农村卫生,2016(12):3.
[4]桂红武.细胞蜡块免疫组化检测在胸腔积液细胞蜡块病理检测中的应用探讨[J].世界最新医学信息文摘,2017,17(28)66,71.
[5]谢刚,原庆会,柯琦,等.免疫组化在老年胸腔积液细胞蜡块病理诊断中的应用[J].四川医学,2015(7):935-937.
[6]李中祥.细胞蜡块切片免疫组化染色在胸腔积液病理诊断中的应用价值[J].河南医学研究,2015(4):88-89.
[7]林静,李美琼,农林琳.胸腔积液细胞蜡块联合免疫组化在肺腺癌与间皮细胞增生鉴别诊断中的应用[J].现代医药卫生,2016(3):338-339.
[8]黄其文,姜汉国.胸腔积液细胞蜡块切片免疫组化染色技术鉴别诊断肺腺癌的临床研究[J].临床肺科杂志,2017,22(2):227-229.
[9]蒙秋萍,薛逢贵,罗志飞,等.胸腔积液细胞蜡块技术在分子病理检测上的应用价值[J].世界最新医学信息文摘,2017(45):228-229.
[10]黄晓萍.肺腺癌恶性胸腔积液细胞蜡块的诊断价值及表皮生长因子受体表达的临床意义[J].中国基层医药,201825(16):2049.
[11]钱红,钟鹏,赵娟霞,等.多种细胞学方法联合检查不明原因渗出性胸腔积液的诊断价值[J].中国实验诊断学,201822(3):11-12.