小儿止泻巴布剂体外抑菌及皮肤刺激性试验
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摘要
目的对小儿止泻巴布剂进行体外抑菌及皮肤刺激性试验,为其临床应用提供实验依据。方法采用平皿法观察小儿止泻巴布剂浸膏对大肠埃希菌、金黄色葡萄球菌、铜绿假单孢杆菌的抑菌效果从而确定最低抑菌浓度;应用新西兰兔完整皮肤及破损皮肤进行试验,考察小儿止泻巴布剂对新西兰兔皮肤刺激性反应。结果小儿止泻巴布剂最低抑菌浓度(MIC):金黄色葡萄球菌100mg/m L,大肠埃希菌400mg/m L,铜绿假单孢杆菌800mg/m L。新西兰兔完整皮肤组多次给药后两侧皮肤无红斑水肿,破损皮肤组出现轻度红斑及轻度水肿。结论小儿止泻巴布剂有一定的抑菌作用,对新西兰兔完整皮肤无刺激性,对破损皮肤有轻度刺激性。
Objective To investigate the antibacterial effect and skin irritation test of Xiao'er Zhixie cataplasm in vitro to provide experimental basis for its clinical application. Methods Xiao'er Zhixie cataplasm was extracted by culture dish method and cocultured with Escherichia Coli, Staphylococcus Aureus, and Pseudomonas Aeruginosa to determine the minimum inhibitory concentrations(MICs). New Zealand rabbits were divided into intact skin group and damaged skin group to test irritation reaction with Xiao'er Zhixie cataplasm. Results The MICs of Xiao'er Zhixie cataplasm on Escherichia Coli, Staphylococcus Aureus, and Pseudomonas Aeruginosa were 100, 400, and800 mg/m L, respectively. No erythema and edema was observed on both sides skin of New Zealand rabbits in the intact skin group after multiple dosing, while the damaged skin group appeared mild erythema and edema. Conclusion Xiao'er Zhixie cataplasm has some antibacterial effect, no irritation to rabbits' intact skin, and mild irritation to damaged skin.
Objective To investigate the antibacterial effect and skin irritation test of Xiao'er Zhixie cataplasm in vitro to provide experimental basis for its clinical application. Methods Xiao'er Zhixie cataplasm was extracted by culture dish method and cocultured with Escherichia Coli, Staphylococcus Aureus, and Pseudomonas Aeruginosa to determine the minimum inhibitory concentrations(MICs). New Zealand rabbits were divided into intact skin group and damaged skin group to test irritation reaction with Xiao'er Zhixie cataplasm. Results The MICs of Xiao'er Zhixie cataplasm on Escherichia Coli, Staphylococcus Aureus, and Pseudomonas Aeruginosa were 100, 400, and800 mg/m L, respectively. No erythema and edema was observed on both sides skin of New Zealand rabbits in the intact skin group after multiple dosing, while the damaged skin group appeared mild erythema and edema. Conclusion Xiao'er Zhixie cataplasm has some antibacterial effect, no irritation to rabbits' intact skin, and mild irritation to damaged skin.
引文
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[3]陈卫平,宾琳,杨胜辉.黄白痤疮膏体外抑菌实验[J].浙江中西医结合杂志,2014,24(2):114-115.
[4]马冬梅,陶庆春,齐宏伟.中药双黄连对泛耐药鲍曼不动杆菌的体外抑菌实验研究[J].实用临床医药杂志,2014,18(16):109-111.
[5]王树梅.小儿止泻膏急性毒性及皮肤刺激性试验[J].中医儿科杂志,2012,8(1):10-12.
[6]姚譞,沈侃,刘然,等.关节止痛膏橡胶膏与热熔压敏胶剂型的体外透皮特性、药效以及皮肤刺激性比较[J].中草药,2013,44(4):443-448.
[7]邱洪,唐旭东,王慧,等.复方冰甲乳膏皮肤刺激性研究[J].中国药房,2014,25(23):2119-2120.
[8]刘慧民,陈向齐,刘玉明,等.复方苦参酊剂对家兔皮肤刺激性研究[J].中国中西医结合皮肤性病学杂志,2014,13(3):154-156.
[9]杨超,唐桂英,吕姗珊,等.麝香追风止痛膏局部皮肤刺激性及过敏性实验研究[J].中国中医急诊,2014,23(8):1491-1493.
[10]蒋婕,郭抗萧,龙玲,等.超微七味白术散体外抑菌作用的研究[J].中国中医药信息杂志,2013,20(11):28-30.
[11]药物刺激性、过敏性和溶血性研究技术指导原则课题研究组.药物刺激性、过敏性和溶血性研究技术指导原则[S].北京:国家食品药品监督管理局,2014,5:63-65.