不同时期人参、西洋参根中单体皂苷Rg_1、Re、Rb_1、Rb_2含量的比较
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摘要
采用超高效液相色谱法测定了不同年生、不同时期的人参和西洋参根中Rg1、Re、Rb1、Rb2 4种主要单体皂苷的含量。结果表明,同年生、同生育期人参根中皂苷Rg1、Rb2的含量均高于西洋参根,皂苷Re、Rb1的含量低于西洋参根;3年生红果期人参根中皂苷Rg1、Rb1、Rb2含量低于3年生绿果期人参根,皂苷Re、Rb1、Rb2含量高于2年生红果期人参。3年生红果期西洋参根中皂苷Rg1、Rb1含量均低于3年生绿果期西洋参根,皂苷Re、Rb2含量高于3年生绿果期西洋参,皂苷Rg1、Rb1、Rb2含量均高于2年生红果期西洋参。
Four kinds of monomer saponin contents of Panax ginseng and American ginseng in different growth periods were determined by ultra performance liquid chromatography. The results showed that the content of ginseng root saponins Rg1, Rb2 were higher than American ginseng, while Re, Rb1 less than American ginseng root in the same year with the growth. The content of ginseng root saponins Re, Rb1, Rb2 of 3-year-old with the same growth periods were more than 2 years old, and Rg1, Rb1, Rb2 of 3 years old red fruit ginseng were lower than the 3-year-old green fruit of ginseng root. The content of American ginseng root saponins Rg1, Rb1 of 3-year-old red fruit were more than 3 years old green fruit, while Re, Rb2 of 3 years old red fruit were lower than the 3-year-old green fruit, and the saponins Rg1, Rb1, Rb2 were more than 2-year-old American ginseng red fruit.
Four kinds of monomer saponin contents of Panax ginseng and American ginseng in different growth periods were determined by ultra performance liquid chromatography. The results showed that the content of ginseng root saponins Rg1, Rb2 were higher than American ginseng, while Re, Rb1 less than American ginseng root in the same year with the growth. The content of ginseng root saponins Re, Rb1, Rb2 of 3-year-old with the same growth periods were more than 2 years old, and Rg1, Rb1, Rb2 of 3 years old red fruit ginseng were lower than the 3-year-old green fruit of ginseng root. The content of American ginseng root saponins Rg1, Rb1 of 3-year-old red fruit were more than 3 years old green fruit, while Re, Rb2 of 3 years old red fruit were lower than the 3-year-old green fruit, and the saponins Rg1, Rb1, Rb2 were more than 2-year-old American ginseng red fruit.
引文
[1]邱楠楠,刘金平,艾民,等.西洋参茎叶化学成分及生物利用度的研究[J].天然产物研究与开发,2012,24(10):1393-1397.
[2]李红艳,张惠,娄军芳,等.不同生长时期人参中6种皂苷含量的变化[J].中华中医药杂志,2015,(2):432-434.
[3]刘胜群,徐海波,任跃英.人参单体皂苷动态变化分析[J].扬州大学学报:农业与生命科学版,2010,31(3):55-59.
[4]Qi L W,Wang C Z,Yuan C S.Ginsenosides from American ginseng:Chemical and pharmacological diversity[J].Phytochemistry,2011,72(8):689-699.
[5]杨金玲,高丽丽,朱平.人参皂苷生物合成研究进展[J].药学学报,2013,48(2):170-178.
[6]刘佳.吉林产区人参、西洋参化学和蛋白差异性对比研究[D].北京:北京中医药大学,2013.
[7]李向高,张连学,孟祥颖,等.西洋参特有成分-拟皂苷F11的分离、鉴定与含量测定[J].吉林农业大学学报,2005,27(6):645-648.
[8]金岩,刘闺男.皂苷Rg1对急性心肌梗死大鼠血管新生的作用[J].中国医科大学学报,2007,36(5):517-519.
[9]郜玉钢,郝建勋,臧埔,等.高效液相色谱法测定农田人参中9种皂苷单体含量[J].食品科学,2012,33(2):189-193.
[10]王红宁.人参皂苷Rb1、Rg1、Re对K562细胞、KG1细胞增殖影响及信号转导分子机制研究[D].重庆:重庆医科大学,2010.
[11]徐成路,张武义,王玉方,等.人参红皮病发病指数对单体皂苷Rb1,Rb2,Re,Rg1的影响[J].人参研究,2016,(4):10-13.
[12]逄世峰,李亚丽,许世泉,等.人参不同部位人参皂苷类成分研究[J].人参研究,2015,27(1):5-8.
[13]孙海,张亚玉,孙长伟,等.不同生长模式下人参土壤养分状况与人参皂苷含量的关系[J].西北农业学报,2012,21(8):146-152.
[14]Sun B S,Xu M Y,Li Z,et al.UPLC-Q-TOF-MS/MS Analysis for Steaming Times-dependent Profiling of Steamed Panax quinquefolius and Its Ginsenosides Transformations Induced by Repetitious Steaming[J].Journal of Ginseng Research,2012,36(3):277-290.
[15]Sollorz G.Quality evaluation of ginseng roots.Quantitative HPLC determination of ginsenosides[J].Deutsch.Apoth.Ztg,1985,125:2052-2055.
[16]李翔国,全炳武,李虎林,等.人参皂苷量变异的研究进展[J].中草药,2012,43(11):2300-2304.
[17]李向高,帅绯.人参、西洋参、人参三七中无机元素的比较[J].中草药,1986,17(10):10-12.
[18]许传莲,郭若薇,郑友兰,等.不同年生西洋参中皂甙的比较研究[J].吉林农业大学学报,1991,(2):88-89.
[19]刘万海,江流,张崇禧,等.不同产地人参一般组成成分分析[J].林业实用技术,1990,(3):12-14.
[20]石威.不同生长期人参中化学成分及农药残留的研究[D].长春:吉林大学,2007.
[21]许云章,任烨,王静霞,等.不同生长年限、不同采收月份加拿大原产地西洋参根中9种皂苷和2种拟皂苷含量动态变化研究[J].中药材,2014,37(10):1743-1748.
[22]谢彩香,索风梅,贾光林,等.皂苷与生态因子的相关性[J].生态学报,2011,31(24):7551-7563.
[23]Choi J E,Li X,Han Y H,et al.Changes of saponin contents of leaves,stems and flower-buds of Panax ginseng CA Meyer by harvesting days[J].Korean Journal of Medicinal Crop Science,2009,17(4):251-256.
[24]吴秀丽,张怡轩,赵文倩,等.真菌EST-Ⅰ及EST-Ⅱ对人参皂苷Rg1定向转化为人参皂苷F1的研究[J].沈阳药科大学学报,2008,25(1):73-76.
[25]张薇,孙晓东,张萍,等.专一转化人参二醇类皂苷Rb1为Rd的真菌菌株的筛选[J].菌物学报,2011,30(2):305-311.
[26]白龙律,臧蕴霞,尹成日.微生物转化人参皂苷Rb1为Rg3的研究[J].延边大学学报:自然科学版,2009,35(2):141-144.
[27]国家药典委员会.中国药典:一部[K].北京:中国医药科技出版社,2015:8-9.
[2]李红艳,张惠,娄军芳,等.不同生长时期人参中6种皂苷含量的变化[J].中华中医药杂志,2015,(2):432-434.
[3]刘胜群,徐海波,任跃英.人参单体皂苷动态变化分析[J].扬州大学学报:农业与生命科学版,2010,31(3):55-59.
[4]Qi L W,Wang C Z,Yuan C S.Ginsenosides from American ginseng:Chemical and pharmacological diversity[J].Phytochemistry,2011,72(8):689-699.
[5]杨金玲,高丽丽,朱平.人参皂苷生物合成研究进展[J].药学学报,2013,48(2):170-178.
[6]刘佳.吉林产区人参、西洋参化学和蛋白差异性对比研究[D].北京:北京中医药大学,2013.
[7]李向高,张连学,孟祥颖,等.西洋参特有成分-拟皂苷F11的分离、鉴定与含量测定[J].吉林农业大学学报,2005,27(6):645-648.
[8]金岩,刘闺男.皂苷Rg1对急性心肌梗死大鼠血管新生的作用[J].中国医科大学学报,2007,36(5):517-519.
[9]郜玉钢,郝建勋,臧埔,等.高效液相色谱法测定农田人参中9种皂苷单体含量[J].食品科学,2012,33(2):189-193.
[10]王红宁.人参皂苷Rb1、Rg1、Re对K562细胞、KG1细胞增殖影响及信号转导分子机制研究[D].重庆:重庆医科大学,2010.
[11]徐成路,张武义,王玉方,等.人参红皮病发病指数对单体皂苷Rb1,Rb2,Re,Rg1的影响[J].人参研究,2016,(4):10-13.
[12]逄世峰,李亚丽,许世泉,等.人参不同部位人参皂苷类成分研究[J].人参研究,2015,27(1):5-8.
[13]孙海,张亚玉,孙长伟,等.不同生长模式下人参土壤养分状况与人参皂苷含量的关系[J].西北农业学报,2012,21(8):146-152.
[14]Sun B S,Xu M Y,Li Z,et al.UPLC-Q-TOF-MS/MS Analysis for Steaming Times-dependent Profiling of Steamed Panax quinquefolius and Its Ginsenosides Transformations Induced by Repetitious Steaming[J].Journal of Ginseng Research,2012,36(3):277-290.
[15]Sollorz G.Quality evaluation of ginseng roots.Quantitative HPLC determination of ginsenosides[J].Deutsch.Apoth.Ztg,1985,125:2052-2055.
[16]李翔国,全炳武,李虎林,等.人参皂苷量变异的研究进展[J].中草药,2012,43(11):2300-2304.
[17]李向高,帅绯.人参、西洋参、人参三七中无机元素的比较[J].中草药,1986,17(10):10-12.
[18]许传莲,郭若薇,郑友兰,等.不同年生西洋参中皂甙的比较研究[J].吉林农业大学学报,1991,(2):88-89.
[19]刘万海,江流,张崇禧,等.不同产地人参一般组成成分分析[J].林业实用技术,1990,(3):12-14.
[20]石威.不同生长期人参中化学成分及农药残留的研究[D].长春:吉林大学,2007.
[21]许云章,任烨,王静霞,等.不同生长年限、不同采收月份加拿大原产地西洋参根中9种皂苷和2种拟皂苷含量动态变化研究[J].中药材,2014,37(10):1743-1748.
[22]谢彩香,索风梅,贾光林,等.皂苷与生态因子的相关性[J].生态学报,2011,31(24):7551-7563.
[23]Choi J E,Li X,Han Y H,et al.Changes of saponin contents of leaves,stems and flower-buds of Panax ginseng CA Meyer by harvesting days[J].Korean Journal of Medicinal Crop Science,2009,17(4):251-256.
[24]吴秀丽,张怡轩,赵文倩,等.真菌EST-Ⅰ及EST-Ⅱ对人参皂苷Rg1定向转化为人参皂苷F1的研究[J].沈阳药科大学学报,2008,25(1):73-76.
[25]张薇,孙晓东,张萍,等.专一转化人参二醇类皂苷Rb1为Rd的真菌菌株的筛选[J].菌物学报,2011,30(2):305-311.
[26]白龙律,臧蕴霞,尹成日.微生物转化人参皂苷Rb1为Rg3的研究[J].延边大学学报:自然科学版,2009,35(2):141-144.
[27]国家药典委员会.中国药典:一部[K].北京:中国医药科技出版社,2015:8-9.