不同年生凤丹根际AM真菌与土壤酶活性的相关性分析
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摘要
利用碱解离-酸性品红方法测定凤丹根样AM真菌结构定殖情况,同时进行土壤菌丝含量、土壤孢子密度及土壤酶活性的测定。结果表明,全部根样均被AM真菌侵染,并形成了典型Arum-type类型的AM结构;AM真菌定殖率、菌丝定殖率、丹皮酚含量及孢子密度在不同年生呈现出相同趋势,均表现为3年生凤丹<4年生凤丹<2年生凤丹,而泡囊定殖率与菌丝圈定殖率呈现出相同趋势,表现为3年生凤丹<2年生凤丹<4年生凤丹;酸性磷酸酶、脲酶、蔗糖酶、多酚氧化酶的活性与土壤菌丝含量呈正相关,酸性磷酸酶、脲酶、蔗糖酶、多酚氧化酶的活性与泡囊定殖率呈负相关,孢子密度和菌丝圈定殖率与酸性磷酸酶、蔗糖酶、多酚氧化酶的活性呈正相关,与脲酶活性呈负相关;菌丝定殖率、AM真菌定殖率与酸性磷酸酶的活性呈正相关,与脲酶、蔗糖酶、多酚氧化酶的活性呈负相关。揭示了在休眠期,不同年生(2、3、4年生)的凤丹根际AM真菌(arbuscular mycorrhizae fungi,AM真菌)共生状况、根部丹皮酚含量测定及土壤酶活性变化。
The method of alkaline hydrolys acid fuchsin staining was used for determining the colonization of AM fungi in the root of Paeonia ostii. The content of soil hypha,spore density in soil and the activity of soil enzyme were also determined. The results showed that all roots samples were infected by AM fungi and formed the structure of Arum-type AM. AM fungal colonization rate in Triennial Paeonia ostii is smaller than the four-year-old Paeonia ostii's which was smaller than the Biennial Paeonia ostii' s. Hypha colonization rate,content of paeonol and spore density in different years' root samples showed the same trend as the AM fungal colonization rate.The colonization of vesicle in Triennial Paeonia ostii was smaller than that in Biennial Paeonia ostii's,the biggest one was four-year-old Paeonia ostii's. The colonization of hyphal coil showed the same trend as the colonization of vesicle. The activity of acid phosphatase,urease,sucrase and polyphenol oxidase was positively correlated with the content of soil hypha and negatively correlated with the colonization of Vesicle. The activity of acid phosphatase,sucrase and polyphenol oxidase was positively correlated with the colonization of hyphae circle and spore density,but negatively correlated with the urease activity. The activity of acid phosphatase was positively correlated with the colonization of hypha and AM fungi,but negatively correlated with the urease,sucrase and polyphenol oxidase's activity.The research reveals the symbiotic relationship of AM fungi with the different year-old root of Paeonia ostii during period of dormancy and determinates the content of cortex moutan phenol and the activity of soil enzyme.
The method of alkaline hydrolys acid fuchsin staining was used for determining the colonization of AM fungi in the root of Paeonia ostii. The content of soil hypha,spore density in soil and the activity of soil enzyme were also determined. The results showed that all roots samples were infected by AM fungi and formed the structure of Arum-type AM. AM fungal colonization rate in Triennial Paeonia ostii is smaller than the four-year-old Paeonia ostii's which was smaller than the Biennial Paeonia ostii' s. Hypha colonization rate,content of paeonol and spore density in different years' root samples showed the same trend as the AM fungal colonization rate.The colonization of vesicle in Triennial Paeonia ostii was smaller than that in Biennial Paeonia ostii's,the biggest one was four-year-old Paeonia ostii's. The colonization of hyphal coil showed the same trend as the colonization of vesicle. The activity of acid phosphatase,urease,sucrase and polyphenol oxidase was positively correlated with the content of soil hypha and negatively correlated with the colonization of Vesicle. The activity of acid phosphatase,sucrase and polyphenol oxidase was positively correlated with the colonization of hyphae circle and spore density,but negatively correlated with the urease activity. The activity of acid phosphatase was positively correlated with the colonization of hypha and AM fungi,but negatively correlated with the urease,sucrase and polyphenol oxidase's activity.The research reveals the symbiotic relationship of AM fungi with the different year-old root of Paeonia ostii during period of dormancy and determinates the content of cortex moutan phenol and the activity of soil enzyme.
引文
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[24]许庆龙,刘晓敏,徐小兵,等.4种丛枝菌根真菌对南高丛蓝莓抗旱性的影响[J].浙江大学学报(农业与生命科学版),2016,42(4):427-434.
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[28]关松荫.土壤酶及其研究法[M].北京:农业出版社,1986:45-47.
[29]杨安娜,李凌飞,赵之伟.滑桃木的丛枝菌根[J].云南大学学报(自然科学版),2004,26(1):85-87.
[30]JUMPONEN A.Dark septate endophytes-Are they mycorrhizal[J].Mycorrhiza,2001,11(4):207-211.
[31]贺学礼,陈程,何博.北方两省农牧交错带沙棘根围AM真菌与球囊霉素空间分布[J].生态学报,2011,31(6):1653-1661.
[2]韩宏毅,王剑.多不饱和脂肪酸及其生理功能[J].中国临床研究,2010,23(6):523-525.
[3]刘立新.ω-3脂肪酸对高血压合并颈动脉粥样硬化患者血管内皮功能的影响[J].中国临床药理学杂志,2010,26(5):330-333.
[4]杨振晶.植物生长物质对‘凤丹’牡丹生根率及幼苗生长的影响[D].聊城:聊城大学,2016.
[5]吴国成.牡丹籽油的提取工艺及主要脂肪酸组成[J].中国化工贸易,2015,7(33):388.
[6]潘法柱.南陵凤丹生物产业基地升为“国家级”[J].安徽林业科技,2014(2):8.
[7]刘润进,陈应龙.菌根学[M].北京:科学出版社,2007:1-447.
[8]白祯.基质和AM真菌对酸枣幼苗生长及营养的影响[D].重庆:西南大学,2013.
[9]乔亚君.AM真菌与施硒量对丹参幼苗生长及品质的影响[D].保定:河北大学,2015.
[10]SURYANARAYANAN T S,MURALI T S,VENKATESAN G.Occurrence and distrbution of fungal endphytes in tropical for ests across a rain fall gradient[J].Can J Bot,2002,80(8):818-826.
[11]于二敏,李聪,贺超兴.AM真菌对大棚芦笋矿质元素吸收和营养品质的影响[C].乌鲁木齐:中国设施园艺学术年会,2014.
[12]贺超,陈伟燕,贺学礼,等.不同水肥因子与AM真菌对黄芩生长和营养成分的交互效应[J].生态学报,2016,36(10):2798-2806.
[13]陈伟燕.不同水肥条件下AM真菌对黄芩生长的影响[D].保定:河北大学,2014.
[14]ABDESSAMAD T A,LINGUA G,AVROVA A.A cullin gene is induced in tomato roots forming arbuscular mycorrhizae[J].Can JBot,2002,80(6):607-616.
[15]鲍长余.三叉苦化学成分和其他两种药用植物有效成分含量测定的研究[D].海口:海南南师范大学,2012.
[16]仇惠君,曾富兰,曾燕红,等.AM真菌对黄花蒿养分吸收和有效成分积累的效应[C].南昌:中国植物学会会员代表大会暨八十周年学术年会,2013.
[17]黄京华,谭钜发,揭红科,等.丛枝菌根真菌对黄花蒿生长及药效成分的影响[J].应用生态学报,2011,22(6):1443-1449.
[18]李建恒.不同因素对丹参等药用植物化学成分的影响研究[D].保定:河北大学,2014.
[19]王平,贺学礼,马丽,等.3种杀真菌剂对AM真菌侵染和黄芩生长的影响[C].重庆:全国菌根学术研讨会,2011.
[20]贺学礼,王凌云,马晶,等.河北省安国地区丹参根围AM真菌多样性[J].生物多样性,2010,18(2):175-181.
[21]秦燕,何峰,仝宗永,等.刈割对羊草草原土壤酶活性和养分含量的影响[J].草业学报,2016,25(4):55-62.
[22]LI Y P,FENG Y L,CHEN Y J,et al.Soil microbes alleviate allelopathy of invasive plants[J].Science Bulletin,2015,60(12):1083-1091.
[23]欧洪,郭冬琴,林俊杰,等.AM真菌对滇重楼根际土壤微生物数量及酶活性的影响[J].中药材,2016,39(5):948-955.
[24]许庆龙,刘晓敏,徐小兵,等.4种丛枝菌根真菌对南高丛蓝莓抗旱性的影响[J].浙江大学学报(农业与生命科学版),2016,42(4):427-434.
[25]BERCH S M,KENDRICK B.Vesicular-arbuscular mycorrhizae of southern ontario ferns and fern-allies[J].Mycologia,1982,74:769-776.
[26]MCGONIGLE T P,MILLER M H,EVANS D G.A new method which gives an objective measure of colonization of roots by vesicular-arbuscular mycorrhizal fungi[J].New Phytologist,1990,115(3):495-501.
[27]KOSKE R E,WALKER C.Gigaspora erytyropa,a new species forming arbuscular mycorrhizae[J].Mycologia,1984,76(2):250-255.
[28]关松荫.土壤酶及其研究法[M].北京:农业出版社,1986:45-47.
[29]杨安娜,李凌飞,赵之伟.滑桃木的丛枝菌根[J].云南大学学报(自然科学版),2004,26(1):85-87.
[30]JUMPONEN A.Dark septate endophytes-Are they mycorrhizal[J].Mycorrhiza,2001,11(4):207-211.
[31]贺学礼,陈程,何博.北方两省农牧交错带沙棘根围AM真菌与球囊霉素空间分布[J].生态学报,2011,31(6):1653-1661.