复方蜥蜴散不同微粒组合剂对胃癌前病变大鼠IKKβ、VEGF表达的影响
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摘要
目的:研究复方蜥蜴散不同微粒组合剂(简称FFXY)治疗对胃癌前病变(PLGC)大鼠凋亡相关蛋白IKKβ及血管内皮生长因子(VEGF)表达的影响,揭示其治疗PLGC的可能作用机制。方法:将120只SPF级SD大鼠随机分为6组,各治疗组均采取单功能烷化剂甲基硝基亚硝基胍(MNNG)配合饥饱失常及情绪刺激综合造模,空白组正常供应标准饲料及清洁蒸馏水。造模成功后分别作相应处理,应用免疫组化法检测胃黏膜组织IKKβ、VEGF蛋白的表达,并对实验结果进行统计分析。结果:IKKβ、VEGF两种蛋白的表达情况,模型组阳性表达高于空白对照组(P<0.01);其他各治疗组阳性表达均低于模型对照(P<0.05);FFXY各治疗组阳性表达均低于维酶素治疗组(P<0.05);80目100目等量混合组两种因子阳性表达低于80目组和100目组(P<0.05)。结论:FFXY各组和维酶素组治疗PLGC均有效,其中,FFXY各组对PLGC大鼠疗效均优于维酶素组,尤以80目100目等量混合剂量组疗效最显著。提示复方蜥蜴散不同微粒组合剂对胃黏膜损伤可能具有保护、修复作用,其作用机制可能是通过降低或抑制PLGC组织中IKKβ、VEGF的表达,从而促进细胞凋亡和抑制血管生成来实现的。
Objective:Study the effect of different particle composition of compound lizards powder(FFXY)therapy on expression of IKKβ and VEGF in PLGC model rats,reveals the possible mechanism for the treatment of PLGC. Methods:120 SPF SD rats were randomly divided into 6 groups,the treatment group were taken MNNG with hunger disorders and emotional stimuli comprehensive building,feed and clean distilled water supply blank group standards. Building after a successful make corresponding processing respectively,using immunohistochemical method to detect gastric mucosa tissue IKKβ,the expression of VEGF protein,and the experimental results were analyzed. Results:IKKβ and VEGF protein expression of model group,positive expression is higher than the blank control group(P<0.01);positive expression of the other treatment group were lower than control group(P<0.01);the positive expression of FFXY treatment group were lower than vatacoenayme treatment group(P<0.05);two factor positive expression of 80 mesh100 mesh equivalent mixing group is lower than 80 mesh and 100 mesh group(P<0.05). Conclusion:FFXY groups and vatacoenayme group were effective on treating PLGC,among them,the FFXY group curative effect of PLGC rats were superior than vatacoenayme group,the 80 mesh 100 mesh equivalent mixing group has the most significant curative effect especially. Different particle composition of compound lizards powder may have protection and repair effect on gastric mucosa injury,its mechanism may be through reducing or inhibiting IKKβ,the expression of VEGF in PLGC organization,so as to promote apoptosis and inhibition of angiogenesis.
Objective:Study the effect of different particle composition of compound lizards powder(FFXY)therapy on expression of IKKβ and VEGF in PLGC model rats,reveals the possible mechanism for the treatment of PLGC. Methods:120 SPF SD rats were randomly divided into 6 groups,the treatment group were taken MNNG with hunger disorders and emotional stimuli comprehensive building,feed and clean distilled water supply blank group standards. Building after a successful make corresponding processing respectively,using immunohistochemical method to detect gastric mucosa tissue IKKβ,the expression of VEGF protein,and the experimental results were analyzed. Results:IKKβ and VEGF protein expression of model group,positive expression is higher than the blank control group(P<0.01);positive expression of the other treatment group were lower than control group(P<0.01);the positive expression of FFXY treatment group were lower than vatacoenayme treatment group(P<0.05);two factor positive expression of 80 mesh100 mesh equivalent mixing group is lower than 80 mesh and 100 mesh group(P<0.05). Conclusion:FFXY groups and vatacoenayme group were effective on treating PLGC,among them,the FFXY group curative effect of PLGC rats were superior than vatacoenayme group,the 80 mesh 100 mesh equivalent mixing group has the most significant curative effect especially. Different particle composition of compound lizards powder may have protection and repair effect on gastric mucosa injury,its mechanism may be through reducing or inhibiting IKKβ,the expression of VEGF in PLGC organization,so as to promote apoptosis and inhibition of angiogenesis.
引文
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[15]牛晓晖,李鹏飞,贾雪婷,等.中国鹿科动物源药材药理作用十年研究进展(二)[J].吉林中医药,2012,32(7):704.
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[2]周本杰,陈蔚文,王建华,等.硝酸结合MNNG攻击大鼠胃癌模型与评价[J].中国药理学通报,2001,7(1):119.
[3]谢晶日,孙芳,梁国英.胃癌前病变动物模型的研究进展[J].中华中医药学刊,2013,30(11):2377.
[4]张颜伟,郭喜军,赵见文.胃癌前病变的中医药研究进展[J].中医学报,2010,27(1):12.
[5]陆晓恒.TLR9-NF-κB信号通路在胃癌组织中的表达及其临床意义[D].济南:山东大学,2012:7.
[6]Kakeji Y,Maehara Y,Sumiyoshi Y,et al.Angiogenesis as a target for gastric cancer[J].Surgery,2002,131:48-54.
[7]Shibata A,Nagaya T,Imai T,et al.Inhibition of NF-kappa B activity decreasesthe VEGF m RNA expression in MDA-MB-231breast cancer cells[J].Breast Cancer Res Treat,2002,73(3):237-243.
[8]Huang S,Robinson JB,Deguzman A,et al.Blockade of nuclear Factor-kappa B signaling inhibits angiogenesis and tumorigenicity of Human ovarian cancer cells by supperssing experssion of vascular en-dothelial growth factor and interleukin 8[J].Cnacer Res,2000,60:5334-5339.
[9]邵冬珊.胃痛日久用鹿角霜[J].中医杂志,2003,44(5):331.
[10]张小梅.黄芪多糖的免疫调节作用及抗肿瘤作用研究进展[J].大连大学学报,2003,24(6):101.
[11]宿廷敏,王敏娟,阮时宝.白术的化学成分及药理作用研究概述[J].贵阳医学学报,2008,2(3):34.
[12]叶华,崔燎.半枝莲多糖的抗肝癌作用及其机制的研究[J].癌症进展杂志,2009,7(3):331-334.
[13]徐敏,卜平,李瑶瑶.半枝莲黄酮类化合物对体外肿瘤血管生成的影响[J].世界华人消化杂志,2007,15(20):2215-2219.
[14]王海波.人参、西洋参和三七抗凝血活性及与药性关系的研究[D].长春:吉林农业大学,2011.
[15]牛晓晖,李鹏飞,贾雪婷,等.中国鹿科动物源药材药理作用十年研究进展(二)[J].吉林中医药,2012,32(7):704.
[16]鲁春梅,张春森,姜立勇.延胡索化学成分及药理作用研究进展[J].中国现代药物应用,2011,5(15):126.