外源NO对康乃馨鲜切花保鲜效果的影响
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摘要
为研究外源NO对康乃馨鲜切花保鲜效果的影响,以康乃馨为试验材料,硝普钠(SNP)为外源NO供体,研究了不同浓度(0,50,100,150,200μmol·L~(-1))SNP处理对康乃馨鲜切花瓶插寿命和花径大小的影响,以此筛选出最佳SNP处理浓度,并测定了最佳SNP处理浓度下,鲜切花花瓣中的可溶性蛋白含量、过氧化物酶(POD)和超氧化物歧化酶(SOD)活性的变化。结果表明:150μmol·L~(-1)的SNP处理康乃馨鲜切花瓶插寿命最长,比对照延长1.53d,延长率为11.57%;与对照相比,第8天花径提升率最高,为70.21%,第16天花径减退率最小,为23.61%。此外,150μmol·L~(-1)SNP处理明显提高了花瓣中的可溶性蛋白含量、POD和SOD活性,有效延缓了康乃馨鲜切花衰老。
In order to study the effect of exogenous NO on the preservation effect of carnation fresh cut flowers,effects of different concentrations of SNP(0,50,100,150,200μmol·L~(-1))on the vase life and the flower diameter of cut carnation were investigated to choose the optimal SNP concentration,using SNP as a donor of exogenous NO.Meanwhile,The changes of soluble protein content,POD and SOD activity in fresh cut flower petals were determined by the optimal SNP concentration.The results showed that the longest vase life was recorded with 150μmol·L~(-1) SNP treatment,which was 1.53 days longer than the control and the elongation rate was11.57%.The highest flower diameter increasing rate(70.21%)was at 8 dand the lowest decreasing rate(23.61%)was at 16 d,compared to the control.In addition,soluble protein content,the activities of POD and SOD were significantly increased with 150μmol·L~(-1) SNP treatment compared to the control.The senescence of fresh cut carnations was effectively delayed with 150μmol·L~(-1) SNP treatment.
In order to study the effect of exogenous NO on the preservation effect of carnation fresh cut flowers,effects of different concentrations of SNP(0,50,100,150,200μmol·L~(-1))on the vase life and the flower diameter of cut carnation were investigated to choose the optimal SNP concentration,using SNP as a donor of exogenous NO.Meanwhile,The changes of soluble protein content,POD and SOD activity in fresh cut flower petals were determined by the optimal SNP concentration.The results showed that the longest vase life was recorded with 150μmol·L~(-1) SNP treatment,which was 1.53 days longer than the control and the elongation rate was11.57%.The highest flower diameter increasing rate(70.21%)was at 8 dand the lowest decreasing rate(23.61%)was at 16 d,compared to the control.In addition,soluble protein content,the activities of POD and SOD were significantly increased with 150μmol·L~(-1) SNP treatment compared to the control.The senescence of fresh cut carnations was effectively delayed with 150μmol·L~(-1) SNP treatment.
引文
[1]姜倩倩,张保仁,汪承建,等.外源硫化氢对康乃馨切花叶绿素荧光参数和抗氧化酶活性的影响[J].北方园艺,2016(23):124-128.
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[11]邱芬,赵根.NO对月季采后超氧化歧化酶的影响[J].浙江农业科学,2013(10):1301-1304.
[12]余细红,曾海燕,杨柳青.SNP对康乃馨切花保鲜的初步研究[J].园艺与种苗,2012(1):44-46.
[13]Zeng C L,Liu L,Xu G Q.The physiological responses of carnation cut flowers to exogenous nitric oxide[J].Scientia Horticulturae,2010,127:424-430.
[14]李如亮.生物化学实验[M].武汉:武汉大学出版社,1998:57-58.
[15]张志良,翟伟著.植物生理学实验指导[M].北京:高等教育出版社,2003.
[16]赵丽,孙百灵,韩立双,等.CaCl2对康乃馨鲜切花保鲜的影响[J].延边大学农学学报,2014,36(4):352-355.
[17]杜兴翠.NO对非洲菊切花衰老的影响及作用机理的研究[D].杭州:浙江农林大学,2012.
[18]杜兴翠,任雪,杨尧,等.外源NO对非洲菊切花保鲜效果及作用机制[J].浙江农业学报,2012,24(3):440-444.
[19]郭彩霞,董艳芳,周媛,等.外源NO对德国鸢尾切花生理指标的影响[J].西北农林科技大学学报(自然科学版),2014,42(11):56-60.
[20]Singh H P,Batish D R,Kaur G,et al.Nitric oxide(as sodium nitropmsside)supplementation ameliorates Cd toxicity in hydroponically grown wheat roots[J].Environmental and Experimental Botany,2008,63:158-167.
[21]李明,王根轩.干旱胁迫对甘草幼苗保护酶活性及脂质过氧化作用的影响[J].生态学报,2002(4):503-507.
[22]Shan L,Chen P Y.The Physiological and Ecological Basis of Dryland Farming[M].Beijing:Science Press,1998:1-18.
[23]Podd L A,Van S J.Physiological response and extension of vase life of cut carnation flowers treated with ethanol and acetaldehyde.Chlorophyll con tent and carbohydrate status[J].Plant Growth Regulation,2002,38:99-105.
[24]曾长立,陈禅友.外源一氧化氮对百合鲜切花的生理效应[J].东北林业大学学报,2011,39(1):46-48.
[25]Bartoli C G,Simontacchi M,Guiamet J J,et al.Antioxidant enzymes and lipid peroxidation during aging of Chrysanthemum morifolium RAM petals[J].Plant Science,1995,104:161-168.
[2]马丽.磷酸钠对康乃馨切花保鲜效果的影响[J].湖北农业科学,2015,54(11):2698-2701,2706.
[3]赵滢,李新凤.6-BA对香石竹切花保鲜效果的研究[J].西北农业学报,2008,17(2):254-257.
[4]任永波,夏晶晖,余前媛,等.保鲜剂对切花康乃馨的生理效应研究[J].成都大学学报:自然科学版,2005,24(3):179-181.
[5]吴文佑,朱天辉.五种抗生素对康乃馨切花保鲜效应[J].四川农业大学学报,2006,24(3):355-359.
[6]Lorenzo L,Garlos G M,Magdalena G,et al.Nitric oxide:The versatility of an extensive signal molecule[J].Annual Review of Plant Biology,2003,54:109-137.
[7]张少颖,饶景萍,任小林.一氧化氮对瓶插月季呼吸作用及相关酶活性的影响[J].园艺学报,2007(1):183-188.
[8]张少颖,饶景萍,高慧.一氧化氮对切花月季瓶插过程中乙烯合成代谢的影响[J].西北农林科技大学学报(自然科学版),2007(11):171-175,180.
[9]Badiyan D,Wills R B H,Bowyer M C.Use of a nitric oxide donor compound to extend the vase life of cut flowers[J].Hortscience,2004,39(6):1371-1372.
[10]杨运英,谭卫萍,余娴陈,等.一氧化氮对非洲菊切花的保鲜效应[J].湖南农业大学学报(自然科学版),2008(3):311-313.
[11]邱芬,赵根.NO对月季采后超氧化歧化酶的影响[J].浙江农业科学,2013(10):1301-1304.
[12]余细红,曾海燕,杨柳青.SNP对康乃馨切花保鲜的初步研究[J].园艺与种苗,2012(1):44-46.
[13]Zeng C L,Liu L,Xu G Q.The physiological responses of carnation cut flowers to exogenous nitric oxide[J].Scientia Horticulturae,2010,127:424-430.
[14]李如亮.生物化学实验[M].武汉:武汉大学出版社,1998:57-58.
[15]张志良,翟伟著.植物生理学实验指导[M].北京:高等教育出版社,2003.
[16]赵丽,孙百灵,韩立双,等.CaCl2对康乃馨鲜切花保鲜的影响[J].延边大学农学学报,2014,36(4):352-355.
[17]杜兴翠.NO对非洲菊切花衰老的影响及作用机理的研究[D].杭州:浙江农林大学,2012.
[18]杜兴翠,任雪,杨尧,等.外源NO对非洲菊切花保鲜效果及作用机制[J].浙江农业学报,2012,24(3):440-444.
[19]郭彩霞,董艳芳,周媛,等.外源NO对德国鸢尾切花生理指标的影响[J].西北农林科技大学学报(自然科学版),2014,42(11):56-60.
[20]Singh H P,Batish D R,Kaur G,et al.Nitric oxide(as sodium nitropmsside)supplementation ameliorates Cd toxicity in hydroponically grown wheat roots[J].Environmental and Experimental Botany,2008,63:158-167.
[21]李明,王根轩.干旱胁迫对甘草幼苗保护酶活性及脂质过氧化作用的影响[J].生态学报,2002(4):503-507.
[22]Shan L,Chen P Y.The Physiological and Ecological Basis of Dryland Farming[M].Beijing:Science Press,1998:1-18.
[23]Podd L A,Van S J.Physiological response and extension of vase life of cut carnation flowers treated with ethanol and acetaldehyde.Chlorophyll con tent and carbohydrate status[J].Plant Growth Regulation,2002,38:99-105.
[24]曾长立,陈禅友.外源一氧化氮对百合鲜切花的生理效应[J].东北林业大学学报,2011,39(1):46-48.
[25]Bartoli C G,Simontacchi M,Guiamet J J,et al.Antioxidant enzymes and lipid peroxidation during aging of Chrysanthemum morifolium RAM petals[J].Plant Science,1995,104:161-168.