猪CISH基因过表达对IL-6和TNF-α基因mRNA的表达调控研究
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摘要
为了分析猪细胞因子诱导的含SH2结构域蛋白(CISH)基因对白细胞介素6(IL-6)和α肿瘤坏死因子(TNF-α)等基因表达的调控,深入研究CISH基因的生物学功能,试验采用猪CISH基因过表达质粒pcDNA-CISH瞬时转染PK-15细胞,利用Real-time PCR技术分析了CISH过表达对相关基因表达量的影响。结果表明:在PK-15细胞中,CISH基因过表达并没有引起TLR4、MyD88、NF-κB、IL-1β、IL-6、TNF-α等基因相对表达量的显著改变;细菌脂多糖(LPS)刺激PK-15细胞后,IL-6、TNF-α和TLR4等基因的相对表达量极显著上调(P<0.01);LPS刺激CISH基因过表达的PK-15细胞后,IL-6基因的相对表达量显著上调(P<0.05)。说明在LPS的刺激下,CISH过表达可以上调IL-6基因的相对表达量。
引文
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[3] 查安东,邢桂玲,高晓雯,等.IL6基因内含子3多态性与仔猪腹泻、生长和公猪繁殖性状的关联分析[J].畜牧兽医学报,2017,48(9): 1674-1682.
[4] 牛步月,邢桂玲,查安东,等.猪肿瘤坏死因子α基因5′侧翼区域多态性分析[J].东北农业大学学报,2017,48(3): 17-23.
[5] HU Z Y,YANG J L,WU Y P,et al. Polymorphisms in CISH Gene Are Associated with Persistent Hepatitis B Virus Infection in Han Chinese Population [J].PLoS One,2014,9(6): e100826.
[6] SONG G,RAO H,FENQ B,et al. Association between CISH polymorphisms and spontaneous clearance of hepatitis B virus in hepatitis B extracellular antigen-positive patients during immune active phase[J]. Chin Med J (Engl),2014,127(9): 1691-1695.
[7] TODENDI P F,POSSUELO L G,KLINGER E I,et al. Low-grade inflammation markers in children and adolescents: Influence of anthropometric characteristics and CRP and IL6 polymorphisms[J].Cytokine,2016,88:177-183.
[8] BANDAY M Z,BALKHI H M,SAMEER A S,et al. Strong association of interleukin-6-174G/C promoter single nucleotide polymorphism with a decreased risk of colorectal cancer in ethnic Kashmiri population: A case control study[J].Tumour Biol,2017,39(3):1010428317695940.
[9] MAINTINGUER NORDE M,OKI E,FERREIRA CARIOCA A A,et al. Influence of IL1B,IL6 and IL10 gene variants and plasma fatty acid interaction on metabolic syndrome risk in a cross-sectional population-based study[J]. Clin Nutr,2018,37(2):659-666.
[10] 田国保,曾争,陆海英,等.肿瘤坏死因子α基因启动子区多态性与乙型肝炎病毒感染相关性的荟萃分析[J].世界华人消化杂志,2007,15(6): 580-584.
[11] LISA R L. Molecular biology of thermoregulation-Invited review:Cytokine regulation of fever: studies using gene knockout mice [J]. J Appl Physiol,2002,92(6):2648-2655.
[12] NADERI M,HASHEMI M,REZAEI M,et al. Association of genetic polymorphisms of IFNGR1 with the risk of pulmonary tuberculosis in Zahedan,Southeast Iran[J].Tuberc Res Treat,2015,2015:292505.
[13] NIU B Y,GUO D C,LIU Z R,et al. Characterization of porcine cytokine inducible SH2-containing protein gene and its association with piglet diarrhea traits[J].Asian-Australas J Anim Sci,2017,30(12):1689-1695.
[14] 孙丽,夏日炜,刘颖,等.大白猪肿瘤坏死因子α基因启动子区多态性及其对mRNA表达水平的影响[J].中国畜牧兽医,2015,42(6):1518-1523.
[15] 李腊梅,杨雪梅,伍洋平,等.肝细胞LO2中细胞因子诱导的含CISH基因的表达及其炎性因子调控的研究[J].成都医学院学报,2017,12(1): 44-48.
[16] HU G K,ZHOU R,LIU J,et al. MicroRNA-98 and let-7 confer cholangiocyte expression of cytokine-inducible Src homology 2-containing protein in response to microbial challenge [J]. Immunol,2009,183(3):1617-1624.