丹黄散-纳米银海绵抑制铜绿假单胞菌的效果
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摘要
背景:课题组前期研究发现丹黄散可促进糖尿病足溃疡的愈合,但抑菌性能较差,因此将具有广谱抗菌作用的纳米银与丹黄散有机结合制成了丹黄散-纳米银海绵(以下简称丹银海绵)。目的:观察丹银海绵对铜绿假单胞菌的抑制效果。方法:将含纳米银高、中、低浓度(分别含硝酸银22.22%,16.67%,11.11%)的丹银海绵及高、中、低浓度(分别含硝酸银22.22%,16.67%,11.11%)的纳米银海绵裁剪为直径1cm的圆形规格,均匀分置于涂满铜绿假单胞菌悬液的培养基上;用同规格纸片蘸取亚胺培南-西司他丁水溶液,置于涂满铜绿假单胞菌悬液培养基上,作为阳性对照组;将壳聚糖海绵裁剪为直径1 cm的圆形规格,分置于涂满铜绿假单胞菌悬液的培养基上,作为阴性对照组。各组均置于35℃恒温箱培养24 h,观察比较各组抑菌圈的大小。结果与结论:亚胺培南-西司他丁组对铜绿假单胞菌的抑菌效果优于各浓度纳米银海绵组、各浓度丹银海绵组及壳聚糖海绵组(P<0.05);各浓度纳米银海绵组和各浓度丹银海绵组的抑菌效果优于壳聚糖海绵组(P <0.05);相同硝酸银浓度下,纳米银海绵组和丹银海绵组具有同等的抑菌效果(P> 0.05);不同浓度的纳米银海绵组抑菌效果无差异(P> 0.05);不同浓度的丹银海绵组抑菌效果无差异(P> 0.05);表明丹银海绵对铜绿假单胞菌具有抑制效果,在糖尿病足溃疡治疗中具有独特的优势。
BACKGROUND: Danhuang powder has been recently shown to promote the healing of diabetic foot ulcers, but its antibacterial activity is not satisfactory. Therefore, broad-spectrum antibacterial nanosilver sponge is loaded with Danhuang powder for clinical application.OBJECTIVE: To investigate the antibacterial activity of nanosilver sponge with Danhuang powder against Pseudomonas aeruginosa.METHODS: Nanosilver sponge with Danhuang powder containing 22.22%, 16.67%, 11.11% silver nitrate(Danhuang-nanosilver group) and simple nanosilver sponge containing 22.22%, 16.67%, 11.11% silver nitrate(simple nanosilver group) were cut into round pieces(1 cm in diameter) and placed on Pseudomonas aeruginosa suspension-spread culture medium. Paper sheets of the same size were dipped in the imipenem-cilastatin aqueous solution(imipenem-cilastatin group) and then also positioned on Pseudomonas aeruginosa suspension-spread culture medium for positive controls. For negative controls, chitosan sponge of the same size was used(chitosan group). All tested materials were cultured in an incubator at 35 °C for 24 hours, and the size of the inhibition zone was compared.RESULTS AND CONCLUSION: The antibacterial activity against Pseudomonas aeruginosa in the imipenem-cilastatin group was superior to that in the Danhuang-nanosilver, simple nanosilver, chitosan groups(P < 0.05). The antibacterial activity against Pseudomonas aeruginosa in the Danhuang-nanosilver and simple nanosilver groups was superior to those in the chitosan group(P < 0.05). The antibacterial activity against Pseudomonas aeruginosa was similar between Danhuang-nanosilver and simple nanosilver groups at the same concentration of silver nitrate(P > 0.05). There was no significant difference in the antibacterial activity against Pseudomonas aeruginosa between different concentrations of silver nitrate in the simple nanosilver and Danhuang-nanosilver groups(both P > 0.05). These results suggest that nanosilver sponge with Danhuang powder exhibits antibacterial activity against Pseudomonas aeruginosa, showing unique advantages in the treatment of diabetic foot ulcers.
BACKGROUND: Danhuang powder has been recently shown to promote the healing of diabetic foot ulcers, but its antibacterial activity is not satisfactory. Therefore, broad-spectrum antibacterial nanosilver sponge is loaded with Danhuang powder for clinical application.OBJECTIVE: To investigate the antibacterial activity of nanosilver sponge with Danhuang powder against Pseudomonas aeruginosa.METHODS: Nanosilver sponge with Danhuang powder containing 22.22%, 16.67%, 11.11% silver nitrate(Danhuang-nanosilver group) and simple nanosilver sponge containing 22.22%, 16.67%, 11.11% silver nitrate(simple nanosilver group) were cut into round pieces(1 cm in diameter) and placed on Pseudomonas aeruginosa suspension-spread culture medium. Paper sheets of the same size were dipped in the imipenem-cilastatin aqueous solution(imipenem-cilastatin group) and then also positioned on Pseudomonas aeruginosa suspension-spread culture medium for positive controls. For negative controls, chitosan sponge of the same size was used(chitosan group). All tested materials were cultured in an incubator at 35 °C for 24 hours, and the size of the inhibition zone was compared.RESULTS AND CONCLUSION: The antibacterial activity against Pseudomonas aeruginosa in the imipenem-cilastatin group was superior to that in the Danhuang-nanosilver, simple nanosilver, chitosan groups(P < 0.05). The antibacterial activity against Pseudomonas aeruginosa in the Danhuang-nanosilver and simple nanosilver groups was superior to those in the chitosan group(P < 0.05). The antibacterial activity against Pseudomonas aeruginosa was similar between Danhuang-nanosilver and simple nanosilver groups at the same concentration of silver nitrate(P > 0.05). There was no significant difference in the antibacterial activity against Pseudomonas aeruginosa between different concentrations of silver nitrate in the simple nanosilver and Danhuang-nanosilver groups(both P > 0.05). These results suggest that nanosilver sponge with Danhuang powder exhibits antibacterial activity against Pseudomonas aeruginosa, showing unique advantages in the treatment of diabetic foot ulcers.
引文
[1]Zhang P,Lu J,Jing Y,et al.Global epidemiology of diabetic foot ulceration:a systematic review and meta-analysis.Ann Med.2016;49(2):106-116.
[2]He Y,Qian H,Xu L,et al.Association between estimated glomerular filtration rate and outcomes in patients with diabetic foot ulcers:a3-year follow-up study.Eur J Endocrinol.2017;177(1):41-50.
[3]罗伟,刘高熊,池卫东,等.不同致病菌感染对糖尿病足溃疡患者病情与预后影响分析[J].中华医院感染学杂志,2018,28(10):1511-1514.
[4]项家丽,张杰,王淑敏,等.继发铜绿假单胞菌感染的糖尿病足溃疡患者病情特点及预后分析[J].中华内分泌代谢杂志,2018,34(5):371-376.
[5]幸运,鲁卫平,喻华,等.中国西部地区耐亚胺培南-西司他丁铜绿假单胞菌的分布及耐药特点[J].中国抗生素杂志,2018,43(9):1079-1082.
[6]Subedi D,Vijay AK,Willcox M.Overview of mechanisms of antibiotic resistance in Pseudomonas aeruginosa:an ocular perspective.Clin Exp Optom.2018;101(2):162-171.
[7]Bowling FL,Rashid ST,Boulton AJ.Preventing and treating foot complicationsassociated with diabetes mellitus.Nat Rev Endocrinol.2015;11(10):606-616.
[8]中国医疗保健国际交流促进会糖尿病足病分会.中国糖尿病足诊治指南[J].中华医学杂志,2017,97(4):251-258.
[9]Lim JZ,Ng NS,Thomas C.Prevention and treatment of diabetic foot ulcers.J R Soc Med.2017;110(3):104-109.
[10]Armstrong DG,Boulton AJM,Bus SA.Diabetic Foot Ulcers and Their Recurrence.N Engl J Med.2017;376(24):2367-2375.
[11]中国微循环学会周围血管疾病专业委员会糖尿病足学组.糖尿病足创面修复治疗专家共识[J].中华糖尿病杂志,2018,10(5):305-309.
[12]Armstrong DG,Boulton AJM,Bus SA.Diabetic Foot Ulcers and Their Recurrence.N Engl J Med.2017;376(24):2367-2375.
[13]徐波,杨彩哲,刘朝阳,等.糖尿病足感染患者病原菌分布及其药物敏感试验[J].中华传染病杂志,2016,34(6):344-348.
[14]王安宇,徐寒松,赵胜,等.基因表达谱差异分析丹黄散对糖尿病足创面修复的作用[J].中国组织工程研究与临床康复,2011,15(28):5233-5236.
[15]朱朝军,吕佳康,周贝,等.糖尿病足溃疡外用中药新药临床研究的思考[J].中国临床药理学杂志,2017,33(13):1257-1259.
[16]张超.丹黄散促进糖尿病大鼠皮肤溃疡创面愈合的实验研究[D].贵阳:贵阳中医学院,2014:1-45.
[17]张定安.丹黄散对糖尿病大鼠创面修复作用及CK14、CK15表达的研究[D].贵阳:贵阳中医学院,2014:1-57.
[18]魏良纲.丹黄散对糖尿病足创面再上皮化能力的影响[D].贵阳:贵阳中医学院,2013:1-39.
[19]邸铁涛,张春玲,陈露,等.丹黄散促进糖尿病足溃疡大鼠创面毛细血管新生[J].糖尿病新世界,2017,20(16):165-166,189.
[20]王安宇,乔艺杰,魏良纲.丹黄散外敷治疗糖尿病足的临床疗效观察[J].贵州医药,2011,35(6):505-507.
[21]戴新新,宿树兰,郭盛,等.丹参酮类成分的生物活性与应用开发研究进展[J].中草药,2017,48(7):1442-1448.
[22]高伟,胡添源,郭娟,等.丹参酮合成生物学研究进展[J].中国中药杂志,2015,40(13):2486-2491.
[23]梁伟雯,陈源,梁颖,等.丹参酮胶囊对临床细菌分离株的体外抑菌作用[J].广东医学,2017,38(24):3792-3795.
[24]杨滢.大黄药效成分及其药理活性研究进展[J].中医临床研究,2018,10(5):142-144.
[25]张斯琪,曾臣红,陈冲,等.如意金黄散的现代研究进展[J].中成药,2018,40(2):411-415.
[26]张博,朱建光.浅析大黄在《伤寒杂病论》中的应用及其现代药理机制[J].中医学报,2017,32(5):766-769.
[27]张来宾,吕洁丽,陈红丽,等.当归中苯酞类成分及其药理作用研究进展[J].中国中药杂志,2016,41(2):167-176.
[28]张炳厚.理气药的应用与鉴别(三)[J].中医杂志,2016,57(18):1545-1548.
[29]罗淑敏,蒋丽红,王亚明.松香活性基团的改性及应用研究进展[J].天然产物研究与开发,2015,27(4):737-741.
[30]刘名倬,刘苗星,王小磊,等.纳米银的抗菌作用及其在烧伤治疗中应用的研究进展[J].中华烧伤杂志,2017,33(2):121-125.
[31]Zapata PA,Larrea M,Tamayo L,et al.Polyethylene/silver-nanofiber composites:A material for antibacterialfilms.Mat Sci Eng C.2016;(69):1282-1289.
[32]陈学情,蒋家璇,任志鸿,等.纳米银的抗菌特性及对多重耐药菌株的抗菌作用[J].微生物学报,2017,57(4):539-549.
[33]于晓旭,鲍会静,徐晨,等.纳米银抗菌作用机制:剂量依赖性促进细菌凋亡[J].中国组织工程研究,2015,19(38):6173-6178.
[34]Maryam A,Sakineh MS.Effects of silver nanoparticles on the thermal properties of polyethylene matrix nano-composites.Therm Anal Calori.2017;128(2):1117-1124.
[35]王君君,薛玉英,唐萌.纳米银在医学领域中的抗菌应用及毒性[J].中华烧伤杂志,2016,32(10):631-634.
[36]曹雪玲,陆书来,张东杰,等.纳米银作为抗菌剂的抗菌性能研究[J].吉林化工学院学报,2017,34(11):30-34.
[2]He Y,Qian H,Xu L,et al.Association between estimated glomerular filtration rate and outcomes in patients with diabetic foot ulcers:a3-year follow-up study.Eur J Endocrinol.2017;177(1):41-50.
[3]罗伟,刘高熊,池卫东,等.不同致病菌感染对糖尿病足溃疡患者病情与预后影响分析[J].中华医院感染学杂志,2018,28(10):1511-1514.
[4]项家丽,张杰,王淑敏,等.继发铜绿假单胞菌感染的糖尿病足溃疡患者病情特点及预后分析[J].中华内分泌代谢杂志,2018,34(5):371-376.
[5]幸运,鲁卫平,喻华,等.中国西部地区耐亚胺培南-西司他丁铜绿假单胞菌的分布及耐药特点[J].中国抗生素杂志,2018,43(9):1079-1082.
[6]Subedi D,Vijay AK,Willcox M.Overview of mechanisms of antibiotic resistance in Pseudomonas aeruginosa:an ocular perspective.Clin Exp Optom.2018;101(2):162-171.
[7]Bowling FL,Rashid ST,Boulton AJ.Preventing and treating foot complicationsassociated with diabetes mellitus.Nat Rev Endocrinol.2015;11(10):606-616.
[8]中国医疗保健国际交流促进会糖尿病足病分会.中国糖尿病足诊治指南[J].中华医学杂志,2017,97(4):251-258.
[9]Lim JZ,Ng NS,Thomas C.Prevention and treatment of diabetic foot ulcers.J R Soc Med.2017;110(3):104-109.
[10]Armstrong DG,Boulton AJM,Bus SA.Diabetic Foot Ulcers and Their Recurrence.N Engl J Med.2017;376(24):2367-2375.
[11]中国微循环学会周围血管疾病专业委员会糖尿病足学组.糖尿病足创面修复治疗专家共识[J].中华糖尿病杂志,2018,10(5):305-309.
[12]Armstrong DG,Boulton AJM,Bus SA.Diabetic Foot Ulcers and Their Recurrence.N Engl J Med.2017;376(24):2367-2375.
[13]徐波,杨彩哲,刘朝阳,等.糖尿病足感染患者病原菌分布及其药物敏感试验[J].中华传染病杂志,2016,34(6):344-348.
[14]王安宇,徐寒松,赵胜,等.基因表达谱差异分析丹黄散对糖尿病足创面修复的作用[J].中国组织工程研究与临床康复,2011,15(28):5233-5236.
[15]朱朝军,吕佳康,周贝,等.糖尿病足溃疡外用中药新药临床研究的思考[J].中国临床药理学杂志,2017,33(13):1257-1259.
[16]张超.丹黄散促进糖尿病大鼠皮肤溃疡创面愈合的实验研究[D].贵阳:贵阳中医学院,2014:1-45.
[17]张定安.丹黄散对糖尿病大鼠创面修复作用及CK14、CK15表达的研究[D].贵阳:贵阳中医学院,2014:1-57.
[18]魏良纲.丹黄散对糖尿病足创面再上皮化能力的影响[D].贵阳:贵阳中医学院,2013:1-39.
[19]邸铁涛,张春玲,陈露,等.丹黄散促进糖尿病足溃疡大鼠创面毛细血管新生[J].糖尿病新世界,2017,20(16):165-166,189.
[20]王安宇,乔艺杰,魏良纲.丹黄散外敷治疗糖尿病足的临床疗效观察[J].贵州医药,2011,35(6):505-507.
[21]戴新新,宿树兰,郭盛,等.丹参酮类成分的生物活性与应用开发研究进展[J].中草药,2017,48(7):1442-1448.
[22]高伟,胡添源,郭娟,等.丹参酮合成生物学研究进展[J].中国中药杂志,2015,40(13):2486-2491.
[23]梁伟雯,陈源,梁颖,等.丹参酮胶囊对临床细菌分离株的体外抑菌作用[J].广东医学,2017,38(24):3792-3795.
[24]杨滢.大黄药效成分及其药理活性研究进展[J].中医临床研究,2018,10(5):142-144.
[25]张斯琪,曾臣红,陈冲,等.如意金黄散的现代研究进展[J].中成药,2018,40(2):411-415.
[26]张博,朱建光.浅析大黄在《伤寒杂病论》中的应用及其现代药理机制[J].中医学报,2017,32(5):766-769.
[27]张来宾,吕洁丽,陈红丽,等.当归中苯酞类成分及其药理作用研究进展[J].中国中药杂志,2016,41(2):167-176.
[28]张炳厚.理气药的应用与鉴别(三)[J].中医杂志,2016,57(18):1545-1548.
[29]罗淑敏,蒋丽红,王亚明.松香活性基团的改性及应用研究进展[J].天然产物研究与开发,2015,27(4):737-741.
[30]刘名倬,刘苗星,王小磊,等.纳米银的抗菌作用及其在烧伤治疗中应用的研究进展[J].中华烧伤杂志,2017,33(2):121-125.
[31]Zapata PA,Larrea M,Tamayo L,et al.Polyethylene/silver-nanofiber composites:A material for antibacterialfilms.Mat Sci Eng C.2016;(69):1282-1289.
[32]陈学情,蒋家璇,任志鸿,等.纳米银的抗菌特性及对多重耐药菌株的抗菌作用[J].微生物学报,2017,57(4):539-549.
[33]于晓旭,鲍会静,徐晨,等.纳米银抗菌作用机制:剂量依赖性促进细菌凋亡[J].中国组织工程研究,2015,19(38):6173-6178.
[34]Maryam A,Sakineh MS.Effects of silver nanoparticles on the thermal properties of polyethylene matrix nano-composites.Therm Anal Calori.2017;128(2):1117-1124.
[35]王君君,薛玉英,唐萌.纳米银在医学领域中的抗菌应用及毒性[J].中华烧伤杂志,2016,32(10):631-634.
[36]曹雪玲,陆书来,张东杰,等.纳米银作为抗菌剂的抗菌性能研究[J].吉林化工学院学报,2017,34(11):30-34.