大花蕙兰组织培养技术研究
|
摘要
以大花蕙兰Cymbidium hybridum侧芽为外植体,以N6、B5、CC、MS为基本培养基,设计无机大量元素、无机微量元素、有机物三因素四水平正交试验,探究大花蕙兰丛生芽增殖的最佳基本培养基、生长调节剂浓度配比以及诱导生根的最佳生长调节剂浓度。结果显示,大花蕙兰丛生芽增殖的最佳培养基组合为B5无机大量元素+CC无机微量元素+MS有机物+蔗糖30 g·L~(-1)+琼脂7 g·L~(-1)+活性炭0.5 g·L~(-1)(p H 5.8~6.0),诱导丛生芽增殖的适宜生长调节剂浓度配比为6-BA 4.0 mg·L~(-1)+NAA 0.2 mg·L~(-1),诱导生根的最佳生长调节剂浓度为NAA 1.0 mg·L~(-1)。
To set up a new tissue culture system with the buds of Cymbidium hybridum as tissue explants,N6,B5,CC and MS as basic medium,orthogonal experiment design was carried out with macroelement,microelement and organic matter as three different influencing factors.Results showed that the best medium combination for developing C.hybridum was B5 macroelement + CC microelement + MS organic + sucrose 30 g·L~(-1) + agar 7 g·L~(-1) + activated carbon 0.5 g·L~(-1)(pH 5.8—6.0),the best medium hormone concentration combination for multiple bud proliferation was 6-BA 4.0 mg·L~(-1)+ NAA 0.2 mg·L~(-1),the best hormone concentration for induce in vitro shoot rooting was NAA 1.0 mg·L~(-1).
To set up a new tissue culture system with the buds of Cymbidium hybridum as tissue explants,N6,B5,CC and MS as basic medium,orthogonal experiment design was carried out with macroelement,microelement and organic matter as three different influencing factors.Results showed that the best medium combination for developing C.hybridum was B5 macroelement + CC microelement + MS organic + sucrose 30 g·L~(-1) + agar 7 g·L~(-1) + activated carbon 0.5 g·L~(-1)(pH 5.8—6.0),the best medium hormone concentration combination for multiple bud proliferation was 6-BA 4.0 mg·L~(-1)+ NAA 0.2 mg·L~(-1),the best hormone concentration for induce in vitro shoot rooting was NAA 1.0 mg·L~(-1).
引文
[1]王育选,任建宏,王鹏丽,赵娟.植物激素对大花蕙兰丛生芽增殖与生长的影响[J].山西农业大学学报,2016,36(9):628-632.
[2]樊家荣,孟想想,曹可.大花蕙兰组织快繁技术研究[J].生物学杂志,2014,31(6):99-102.
[3]蔺红萍,李培.大花蕙兰组织培养技术[J].北方园艺,2010(3):138-140.
[4]Morel G M.Producing virns-free Cymbidium[J].American Orchid Society Bulletin,1960(9):495-497.
[5]邹宗兰,秦庭豪,文颖.大花蕙兰工厂化繁殖技术研究[J].西南农业学报,2007,20(4):743-746.
[6]姚丽娟,徐晓薇,陈义增,林绍生.大花蕙兰原球茎增殖分化影响因素研究[J].农业科技通讯,2008(5):58-59.
[7]施隆文,孟祥云,庄应强.大花蕙兰类原球茎增殖与分化影响因素的初步探究[J].北方园艺,2008(4):230-232.
[8]韩明,李晏萍,潘学峰.利用丛生芽增殖途径对大花蕙兰进行离体快繁[J].热带生物学报,2012,3(3):261-266.
[9]Morel G M.Tissue culture--a new means of clonal propagation of orchids[J].American Orchid Society Bulletin,1964,28(1):375-380.
[10]Whimber D E.Clonal multiplicatication of Cymbidium through tissue culture of the shoot meristem[J].American Orchid Society Bulletin,1993,32:105-107.
[11]何红卫.花生再生系统的建立[D].广州:华南师范大学硕士学位论文,2002.
[12]黄学林,李筱菊.高等植物组织离体培养的形态建成及其调控[M].北京:中国农业出版社,1992:65-68.
[2]樊家荣,孟想想,曹可.大花蕙兰组织快繁技术研究[J].生物学杂志,2014,31(6):99-102.
[3]蔺红萍,李培.大花蕙兰组织培养技术[J].北方园艺,2010(3):138-140.
[4]Morel G M.Producing virns-free Cymbidium[J].American Orchid Society Bulletin,1960(9):495-497.
[5]邹宗兰,秦庭豪,文颖.大花蕙兰工厂化繁殖技术研究[J].西南农业学报,2007,20(4):743-746.
[6]姚丽娟,徐晓薇,陈义增,林绍生.大花蕙兰原球茎增殖分化影响因素研究[J].农业科技通讯,2008(5):58-59.
[7]施隆文,孟祥云,庄应强.大花蕙兰类原球茎增殖与分化影响因素的初步探究[J].北方园艺,2008(4):230-232.
[8]韩明,李晏萍,潘学峰.利用丛生芽增殖途径对大花蕙兰进行离体快繁[J].热带生物学报,2012,3(3):261-266.
[9]Morel G M.Tissue culture--a new means of clonal propagation of orchids[J].American Orchid Society Bulletin,1964,28(1):375-380.
[10]Whimber D E.Clonal multiplicatication of Cymbidium through tissue culture of the shoot meristem[J].American Orchid Society Bulletin,1993,32:105-107.
[11]何红卫.花生再生系统的建立[D].广州:华南师范大学硕士学位论文,2002.
[12]黄学林,李筱菊.高等植物组织离体培养的形态建成及其调控[M].北京:中国农业出版社,1992:65-68.