姬玉露组织培养体系的建立
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摘要
以姬玉露叶片为试验材料,研究建立其组织培养体系的方法。结果表明,75%酒精浸泡30 s,0.1%HgCl_2消毒9 min是最适宜的叶片消毒方法;1 mg/L 6-BA+1 mg/L KT+0.1 mg/L NAA为最适诱导愈伤组织培养基,诱导率达87.5%;在MS+0.8 mg/L 6-BA+0.1 mg/L NAA培养基中,丛生芽平均增殖倍数为6.1倍,为最适增殖培养基;MS+0.8 mg/L 6-BA+0.1 mg/L NAA培养基中添加20 g/L马铃薯泥有利于姬玉露壮苗生长;生根培养基为1/2MS+0.03 mg/L IBA+2 g/L活性炭+20 g/L马铃薯泥时,幼苗生根率为86.7%;移栽基质以粗河沙+泥炭(体积比为2∶1)较好,植株生长健壮,新根长度平均为2.69 cm。
The leaves of Haworthia cooperi var.truncata were used for the test material to study the method of establishing its tissue culture.The results showed that the optimal method of leaf disinfection was immersion in 75% ethanol by 30s,disinfection with 0.1%HgCl_2 by 9 min.The optimal medium for callus initiation was 1 mg/L 6-BA+1 mg/L KT+0.1 mg/L NAA,under this medium,achieved 87.5% induction rate.The optimal proliferation medium was MS+0.8 mg/L 6-BA+0.1 mg/L NAA,under this medium,average proliferation rate of multiple shoots was 6.1.It was benefit to the growth of seeds by adding 20 g/L mashed potato in medium of MS+0.8 mg/L 6-BA+0.1 mg/L NAA.Seedling rooting rate achieved 90% in the rooting medium with 1/2 MS+0.03 mg/L IBA+2 g/L activated carbon+20 g/L mashed potato.The transplanting medium with coarse river sand and peat was well for strong growth of plant,length of newroots was 2.69 cm.
The leaves of Haworthia cooperi var.truncata were used for the test material to study the method of establishing its tissue culture.The results showed that the optimal method of leaf disinfection was immersion in 75% ethanol by 30s,disinfection with 0.1%HgCl_2 by 9 min.The optimal medium for callus initiation was 1 mg/L 6-BA+1 mg/L KT+0.1 mg/L NAA,under this medium,achieved 87.5% induction rate.The optimal proliferation medium was MS+0.8 mg/L 6-BA+0.1 mg/L NAA,under this medium,average proliferation rate of multiple shoots was 6.1.It was benefit to the growth of seeds by adding 20 g/L mashed potato in medium of MS+0.8 mg/L 6-BA+0.1 mg/L NAA.Seedling rooting rate achieved 90% in the rooting medium with 1/2 MS+0.03 mg/L IBA+2 g/L activated carbon+20 g/L mashed potato.The transplanting medium with coarse river sand and peat was well for strong growth of plant,length of newroots was 2.69 cm.
引文
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[21]李亮,张冬敏,雷华辉,等.植物组织培养中有机添加物应用研究[J].宁夏农林科技,2012,53(2):28-30.
[2]兑宝峰.热门多肉植物盘点——玉露篇(上)[J].花卉盆景(花卉园艺),2016(1):20-23.
[3]谢维荪,徐民生.多肉花卉品种与栽培[M].北京:中国农业科技出版社,1994:56.
[4]任改婷,张盛圣,张桂芳,等.姬玉露不定芽诱导及高频离体再生体系的建立[M]//张启翔.中国观赏园艺研究进展2016.北京:中国林业出版社,2016:555-560.
[5]陈红刚,高素芳,杨韬.玉露的组织培养与快速繁殖[J].北方园艺,2011(12):101-102.
[6]宋扬.冰灯玉露的组织培养与快速繁殖研究[J].现代农业科技,2014(18):164-166.
[7]孙涛,金蕊,李德森.康平寿的组织培养与快速繁殖[J].植物生理学通讯,2003,39(3):232.
[8]高越,王娅欣,孙涛,等.毛玉露的组织培养与快速繁殖[J].生物学通报,2010,45(6):54-55.
[9]左志宇,李建希,安晓云,等.克里克特寿的组织培养与快速繁殖[J].植物生理学通讯,2007,43(2):311-313.
[10]宋晓涛,沈萌,左志宇,等. 12卷属植物西山寿的组织培养与快速繁殖[J].植物生理学通讯,2007,43(5):883-884.
[11]赵昂.左志宇,宋晓涛,等. Hawoahm mirabilis的组织培养与快速繁殖[J].植物生理学通讯,2007,43(6):1137-1138.
[12]王泉,左志宇,宋晓涛,等.百合科多肉植物美吉寿的组织培养与快速繁殖[J].植物生理学通讯,2008,44(1):123-124.
[13]张吴鹏,宋晓涛,张耀,等.白银寿的组织培养与快速繁殖[J].植物生理学通讯,2008,44(5):951-952.
[14]郭生虎,朱永兴,关雅静.百合科十二卷属玉露的组培快繁关键技术研究[J].中国农学通报,2016,32(34):85-89.
[15]郜李彬,曹征宇,顾韵莉,等.玉露组培诱导不同部位外植体及不同培养基配方筛选初报[J].上海农业科技,2017(5):102-104.
[16]严小峰,刘艳军,黄俊轩,等.冰灯玉露松散型胚性愈伤组织的诱导方法[J].天津农业科学,2017,23(7):21-24.
[17]段鹏慧.花卉组培快繁与脱毒[M].北京:中国农业出版社,2017:48.
[18]曹春英,丁雪珍.植物组织培养[M].北京:中国农业出版社,2014:32.
[19]周鑫.植物组织培养[M].北京:航空工业出版社,2012:29.
[20]梁一池,杨华.植物组织培养技术的研究进展[J].福建林学院学报,2002,22(1):1-3.
[21]李亮,张冬敏,雷华辉,等.植物组织培养中有机添加物应用研究[J].宁夏农林科技,2012,53(2):28-30.