MS-222对东海带鱼幼鱼的麻醉效果研究
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摘要
为研究MS-222麻醉剂对东海带鱼Trichiurus haumela幼鱼的麻醉效果,在水温(26. 0±1. 0)℃条件下,对体质量为(43. 21±0. 52) g的东海带鱼进行了不同MS-222浓度的麻醉试验,根据鱼体在最终麻醉状态和复苏过程中的行为特征,把麻醉程度分为6个时期,复苏过程分为4个时期,测定了麻醉和复苏各期试验鱼的呼吸频率,并利用不同质量浓度的麻醉剂,研究了其对东海带鱼幼鱼的麻醉程度和麻醉效果,以及对血清皮质醇和血糖水平的影响。结果表明:当MS-222麻醉剂浓度低于20 mg/L时,试验鱼的最终麻醉程度为0期;当麻醉剂浓度高于80 mg/L时,试验鱼的最终麻醉程度均能达到6期;试验鱼的入麻时间随着MS-222浓度的升高而减少,复苏时间随MS-222浓度的升高而增加;在本试验条件下,测得的MS-222有效质量浓度为120～140 mg/L;在MS-222浓度为20、40 mg/L条件下,血清皮质醇含量随麻醉时间的延长呈先上升后下降的趋势,在MS-222浓度为60 mg/L条件下,血清皮质醇含量呈逐渐下降的趋势,各浓度组试验鱼在6 h时血清皮质醇含量最低,但无显著性差异(P>0. 05);各试验组鱼血糖含量随麻醉时间的延长呈逐渐下降的趋势,均在6 h时降至最低,也无显著性差异(P>0. 05)。研究表明,MS-222麻醉剂对东海带鱼幼鱼的有效质量浓度为120～140 mg/L,具有良好的麻醉效果。
The respiratory rate,anesthetic process,and concentrations of serum cortisol( COR) and glucose( GLU) were determined in largehead hairtail juvenile Trichiurus haumela with body weight of( 43.21±0.52) g anaesthetized by MS-222 at a dose of 10,20,40,60,80,100,120,140,and 160 mg/L in East China Sea at water temperature of( 26.0±1.0) ℃ to investigate the anesthetic effect of MS-222 on largehead hairtail juveniles. According to the fish behavior characteristics in the final stage of anesthetic and recovery process,the anesthetic process was divided into six periods and the recovery process into four stages. It was found that the final anesthetic degree of0 was observed at MS-222 concentration of below 20 mg/L,and the final anesthetic degree of 6 at MS-222 concentration of over 80 mg/L in all experimental groups. The required time for narcotic state was found to be decreased and the recovery time increased as the concentration of MS-222 was elevated,with the efficient MS-222 mass concentration of 120-140 mg/L under the experimental conditions. The COR contents were increased and then decreased with the prolonged anesthetic period at MS-222 concentration of 20 mg/L and 40 mg/L,and again decrease at MS-222 concentration of 60 mg/L,with the minimal COR content in the fish anaesthetized by MS-222 at6 hours,without significant difference( P > 0. 05). There was low serum GLU content with elapse of anesthetic process,with the minimal value at 6 hours post anesthetic,and without significant difference( P>0.05). In conclusion,it is suggested that the effective mass concentration for largehead hairtail juveniles be 120-140 mg/L of MS-222.
The respiratory rate,anesthetic process,and concentrations of serum cortisol( COR) and glucose( GLU) were determined in largehead hairtail juvenile Trichiurus haumela with body weight of( 43.21±0.52) g anaesthetized by MS-222 at a dose of 10,20,40,60,80,100,120,140,and 160 mg/L in East China Sea at water temperature of( 26.0±1.0) ℃ to investigate the anesthetic effect of MS-222 on largehead hairtail juveniles. According to the fish behavior characteristics in the final stage of anesthetic and recovery process,the anesthetic process was divided into six periods and the recovery process into four stages. It was found that the final anesthetic degree of0 was observed at MS-222 concentration of below 20 mg/L,and the final anesthetic degree of 6 at MS-222 concentration of over 80 mg/L in all experimental groups. The required time for narcotic state was found to be decreased and the recovery time increased as the concentration of MS-222 was elevated,with the efficient MS-222 mass concentration of 120-140 mg/L under the experimental conditions. The COR contents were increased and then decreased with the prolonged anesthetic period at MS-222 concentration of 20 mg/L and 40 mg/L,and again decrease at MS-222 concentration of 60 mg/L,with the minimal COR content in the fish anaesthetized by MS-222 at6 hours,without significant difference( P > 0. 05). There was low serum GLU content with elapse of anesthetic process,with the minimal value at 6 hours post anesthetic,and without significant difference( P>0.05). In conclusion,it is suggested that the effective mass concentration for largehead hairtail juveniles be 120-140 mg/L of MS-222.
引文
[1]张晓敏.带鱼下脚料水解蛋白的制备及其利用研究[D].重庆:西南大学,2008.
[2]刘长琳,何力,陈四清,等.鱼类麻醉研究综述[J].渔业现代化,2007,34(5):21-25.
[3] Mattson N S,Riple T H. Metomidate,a better anesthetic for cod(Gadus morhua)in comparison with benzocaine,MS-222,chlorobutanol,and phenoxyethanol[J].Aquaculture,1989,83(1-2):89-94.
[4]杜浩,危起伟,杨德国,等.MS-222、丁香油、苯唑卡因对养殖美洲鲥幼鱼的麻醉效果[J].大连水产学院学报,2007,22(1):20-26.
[5]田家元,王京树,万建义,等.MS-222不同处理方式对史氏鲟和中华鲟麻醉效果的影响[J].水生态学杂志,2011,32(5):87-90.
[6]刘长琳,陈四清,何力,等.MS-222对半滑舌鳎成鱼的麻醉效果研究[J].中国水产科学,2008,15(1):92-99.
[7] Oikawa S,Takeda T,Itazawa Y.Scale effects of MS-222 on a marine teleost,porgy Pagrus major[J]. Aquaculture,1994,121(4):369-379.
[8] Chambel J,Pinho R,Sousa R,et al.The efficacy of MS-222 as anaesthetic agent in four freshwater aquarium fish species[J]. Aquaculture Research,2015,46(7):1582-1589.
[9] Marking L L,Meyer F P.Are better anesthetics needed in fisheries?[J].Fisheries,1985,10(6):2-5.
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[11] Schoettger R A,Walker C R,Marking L L,et al.MS-222 as an anesthetic for channel catfish:its toxicity,efficacy,and muscle residues[R]. Washington,DC:U. S. Fish and Wildlife Service,1967,17:1-14.
[12] Summerfelt R C,Smith L S. Anaesthesia,surgery,and related techniques[M]//Schreck C B,Moyle P B.Methods for Fish Biology.Bethesda,MD:American Fisheries Society,1990:213-272.
[13]胡发文,李莉,刘元文,等.丁香油和MS-222对大泷六线鱼幼鱼的麻醉效果[J].大连海洋大学学报,2017,32(6):671-675.
[14]李春梅,黄永坚.MS-222麻醉金鱼的研究[J].黄冈职业技术学院学报,2002(4):67-70,63.
[15]张朝晖,丛娇日,王波,等.麻醉剂丁香酚对黄腊鲹耗氧的影响[J].海洋科学,2003,27(6):11-14,34.
[16] Cooke S J,Suski C D,Ostrand K G,et al.Behavioral and physiological assessment of low concentrations of clove oil anaesthetic for handling and transporting largemouth bass(Micropterus salmoides)[J].Aquaculture,2004,239(1-4):509-529.
[17]郭丰红,汪之和.MS-222对鳜鱼成鱼麻醉效果的研究[J].湖南农业科学,2009(7):150-153.
[18]赵明,柳学周,徐永江,等.MS-222麻醉圆斑星鲽成鱼效果研究[J].海洋科学进展,2010,28(4):531-537.
[19]彭宁东,汤文圣,郭栋,等.MS-222对赤眼鳟幼鱼麻醉效果的研究[J].华南师范大学学报:自然科学版,2016,48(6):37-43.
[20]王秀华,张烨伟,杨春志.MS-222对牙鲆麻醉效果[J].渔业科学进展,2009,30(3):1-6.
[21] Barton B A,Peter R E,Paulencu C R. Plasma cortisol levels of fingerling rainbow trout(Salmo gairdneri)at rest,and subjected to handling,confinement,transport,and stocking[J]. Canadian Journal of Fisheries and Aquatic Sciences,1980,37(5):805-811.
[22] Strange R J,Schreck C B,Ewing R D.Cortisol concentrations in confined juvenile Chinook salmon(Oncorhynchus tshawytscha)[J]. Transactions of the American Fisheries Society,1987,107(6):812-819.
[23]梁政远,安丽娜,董在杰,等.丁香油对罗非鱼的麻醉作用及其对血液指标和激素水平的影响[J].上海海洋大学学报,2009,18(5):629-635.
[24]朱挺兵,李飞,吴兴兵,等.电麻醉对圆口铜鱼幼鱼行为及血液生化指标的影响[J].四川动物,2015,34(6):885-888.
[25] Donaldson E M.The pituitary-interrenal axis as an indicator of stress in fish[M]//Pickering A D. Stress and Fish. London:Academic Press,1981:11-48.
[26]冯广朋,徐滨,庄平,等.化学麻醉与电麻醉对史氏鲟幼鱼血液指标的影响[J].安徽农业科学,2010,38(20):10731-10733,10754.
[2]刘长琳,何力,陈四清,等.鱼类麻醉研究综述[J].渔业现代化,2007,34(5):21-25.
[3] Mattson N S,Riple T H. Metomidate,a better anesthetic for cod(Gadus morhua)in comparison with benzocaine,MS-222,chlorobutanol,and phenoxyethanol[J].Aquaculture,1989,83(1-2):89-94.
[4]杜浩,危起伟,杨德国,等.MS-222、丁香油、苯唑卡因对养殖美洲鲥幼鱼的麻醉效果[J].大连水产学院学报,2007,22(1):20-26.
[5]田家元,王京树,万建义,等.MS-222不同处理方式对史氏鲟和中华鲟麻醉效果的影响[J].水生态学杂志,2011,32(5):87-90.
[6]刘长琳,陈四清,何力,等.MS-222对半滑舌鳎成鱼的麻醉效果研究[J].中国水产科学,2008,15(1):92-99.
[7] Oikawa S,Takeda T,Itazawa Y.Scale effects of MS-222 on a marine teleost,porgy Pagrus major[J]. Aquaculture,1994,121(4):369-379.
[8] Chambel J,Pinho R,Sousa R,et al.The efficacy of MS-222 as anaesthetic agent in four freshwater aquarium fish species[J]. Aquaculture Research,2015,46(7):1582-1589.
[9] Marking L L,Meyer F P.Are better anesthetics needed in fisheries?[J].Fisheries,1985,10(6):2-5.
[10]邬国民,何莉,何桂福.我国鱼类麻醉运输的现状及发展前景[J].中山大学学报论丛,1998(4):62-65.
[11] Schoettger R A,Walker C R,Marking L L,et al.MS-222 as an anesthetic for channel catfish:its toxicity,efficacy,and muscle residues[R]. Washington,DC:U. S. Fish and Wildlife Service,1967,17:1-14.
[12] Summerfelt R C,Smith L S. Anaesthesia,surgery,and related techniques[M]//Schreck C B,Moyle P B.Methods for Fish Biology.Bethesda,MD:American Fisheries Society,1990:213-272.
[13]胡发文,李莉,刘元文,等.丁香油和MS-222对大泷六线鱼幼鱼的麻醉效果[J].大连海洋大学学报,2017,32(6):671-675.
[14]李春梅,黄永坚.MS-222麻醉金鱼的研究[J].黄冈职业技术学院学报,2002(4):67-70,63.
[15]张朝晖,丛娇日,王波,等.麻醉剂丁香酚对黄腊鲹耗氧的影响[J].海洋科学,2003,27(6):11-14,34.
[16] Cooke S J,Suski C D,Ostrand K G,et al.Behavioral and physiological assessment of low concentrations of clove oil anaesthetic for handling and transporting largemouth bass(Micropterus salmoides)[J].Aquaculture,2004,239(1-4):509-529.
[17]郭丰红,汪之和.MS-222对鳜鱼成鱼麻醉效果的研究[J].湖南农业科学,2009(7):150-153.
[18]赵明,柳学周,徐永江,等.MS-222麻醉圆斑星鲽成鱼效果研究[J].海洋科学进展,2010,28(4):531-537.
[19]彭宁东,汤文圣,郭栋,等.MS-222对赤眼鳟幼鱼麻醉效果的研究[J].华南师范大学学报:自然科学版,2016,48(6):37-43.
[20]王秀华,张烨伟,杨春志.MS-222对牙鲆麻醉效果[J].渔业科学进展,2009,30(3):1-6.
[21] Barton B A,Peter R E,Paulencu C R. Plasma cortisol levels of fingerling rainbow trout(Salmo gairdneri)at rest,and subjected to handling,confinement,transport,and stocking[J]. Canadian Journal of Fisheries and Aquatic Sciences,1980,37(5):805-811.
[22] Strange R J,Schreck C B,Ewing R D.Cortisol concentrations in confined juvenile Chinook salmon(Oncorhynchus tshawytscha)[J]. Transactions of the American Fisheries Society,1987,107(6):812-819.
[23]梁政远,安丽娜,董在杰,等.丁香油对罗非鱼的麻醉作用及其对血液指标和激素水平的影响[J].上海海洋大学学报,2009,18(5):629-635.
[24]朱挺兵,李飞,吴兴兵,等.电麻醉对圆口铜鱼幼鱼行为及血液生化指标的影响[J].四川动物,2015,34(6):885-888.
[25] Donaldson E M.The pituitary-interrenal axis as an indicator of stress in fish[M]//Pickering A D. Stress and Fish. London:Academic Press,1981:11-48.
[26]冯广朋,徐滨,庄平,等.化学麻醉与电麻醉对史氏鲟幼鱼血液指标的影响[J].安徽农业科学,2010,38(20):10731-10733,10754.