摘要
为了研究抑制Dj-β-catenin-1基因对涡虫再生的影响,构建了L4440-Dj-β-catenin-1干扰载体.将含有重组质粒L4440-Dj-β-catenin-1的HT115细菌经IPTG诱导后直接喂食涡虫.结果表明,Dj-β-catenin-1基因经RNA干扰后涡虫不能正常再生,面向后端伤口再生出一个头部而不是尾.此外,Dj-β-catenin-1基因的沉默还能使涡虫正常的尾转变成头.上述工作为进一步研究Dj-β-catenin-1基因在日本三角涡虫再生中的作用奠定良好基础.
In order to investigate the effects of inhibition of Dj-β-catenin-1 gene on planarian regeneration,the interference vector of L4440-Dj-β-catenin-1 is constructed in this research.After the IPTG induction of dsRNA,the bacteria HT115 containing the recombinant plasmid L4440-Dj β-catenin-1 are directly fed to planarians.The results show that RNA interference(RNAi)of Dj-β-catenin-1 causes the inappropriate regeneration of a head instead of a tail at posterior amputations.In addition,the silencing of Dj-β-catenin-1 is sufficient to transform the normal tail into a head.This work plays a good foundation for further studying the function of Dj-β-catenin-1 gene in planarian regeneration.
引文
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