香附水提液对小麦幼苗生理活性酶的影响
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摘要
鉴于中草药在抗菌、杀菌,抑制细胞分裂等方面的作用,利用中草药来抑制小麦穗发芽或许是一种新的途径和方法。为了初步研究香附水提液在小麦种子萌发过程中对幼苗生理活性酶的影响,采用室内培养皿培养法,分析不同浓度的香附水提液浸种后对小麦幼苗的α-淀粉酶含量、叶绿素含量和过氧化氢酶活性的作用,结果表明α-淀粉酶含量、叶绿素含量随水提液浓度的增加而降低,过氧化氢酶活性含量则随之递增,并且以香附水提液浓度为120 g/L和150 g/L的处理差异显著,此为研究开发抑制小麦穗发芽的中草药生物抑制剂提供试验依据。
In view of the effect of Chinese herbal medicine in antibiosis, sterilization, inhibition of cell division, it may be a new way to suppress the wheat ear sprouting by using Chinese herbal medicine. In order to preliminarily study on the effects of water extract from Rhizoma Cyperi on physiological enzyme activity of wheat seedling during wheat seed germination, we analyzed the influence of different concentrations of water extract from Rhizoma Cyperi after seed soaking on wheat seedling α-amylase content, chlorophyll content and catalase activity with indoor plate culture. The results showed that α-amylase content and chlorophyll content decreased with the increase of the aqueous extracts concentration, while the content of catalase activity increased with the increase of the aqueous extract concentrations. Besides, there were significant differences in the treatment of 120 g/L and 150 g/L concentrations of aqueous extract, which would provide an experimental basis for the research and development of Chinese herbal biological inhibitors to inhibit the germination of wheat spikes.
In view of the effect of Chinese herbal medicine in antibiosis, sterilization, inhibition of cell division, it may be a new way to suppress the wheat ear sprouting by using Chinese herbal medicine. In order to preliminarily study on the effects of water extract from Rhizoma Cyperi on physiological enzyme activity of wheat seedling during wheat seed germination, we analyzed the influence of different concentrations of water extract from Rhizoma Cyperi after seed soaking on wheat seedling α-amylase content, chlorophyll content and catalase activity with indoor plate culture. The results showed that α-amylase content and chlorophyll content decreased with the increase of the aqueous extracts concentration, while the content of catalase activity increased with the increase of the aqueous extract concentrations. Besides, there were significant differences in the treatment of 120 g/L and 150 g/L concentrations of aqueous extract, which would provide an experimental basis for the research and development of Chinese herbal biological inhibitors to inhibit the germination of wheat spikes.
引文
Lai Q.J.,Zhao W.Y.,Li X.L.,and Lv J.Z.,2016,Effects of artemisinin on seed germination and seedling growth of wheat,Mailei Zuowu Xuebao(Journal of Triticeae Crops),36(10):1386-1390(来庆捷,赵婉莹,李雪丽,吕建洲,2016,青篙素对小麦种子萌发及幼苗生长的影响,麦类作物学报,36(10):1386-1390)
Liu J.X.,Hu H.B.,Zhao G.L.,and Wang X.,2006,Effect of alkaloid extract from Peganum multisectum bobr on seed germination and seedling growth of wheat(Triticum aestivum L.),Zhiwu Shenglixue Tongxun(Plant Physiology Communications),42(2):213-216(刘建新,胡浩斌,赵国林,王鑫,2006,多裂骆驼蓬中生物碱类物质对小麦种子萌发和幼苗生长的影响,植物生理学通讯,42(2):213-216)
Lin X.S.,Shi H.Q.,Huang F.,and Huang X.L.,2006,Analysis of essential oil from extraction Cyperus rotundus L.by GC/MS,Zhipu Xuebao(Journal of Chinese Mass Spectrometry Society),27(1):40-44(林晓珊,吴惠勤,黄芳,黄晓兰,2006,香附挥发油的提取和GC/MS分析,质谱学报,27(1):40-44)
Sun G.Z.,Yan C.S.,and Xiao S.H.,2003,The mechanism on wheat pre-harvest sprouting,Zhongguo Nongye Kexue Daobao(Journal of Agricultural Science and Technology),5(6):13-18(孙果忠,闰长生,肖世和,2003,小麦穗发芽机制研究进展,中国农业科技导报,5(6):13-18)
Sun J.Y.,Li S.M.,Tong S.L.,and Liu S.H.,2017,Effect of leach liquor from Rhizoma cyperi on wheat seed germination and seedling growth,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),36(9):3907-3911(孙君艳,李淑梅,仝胜利,刘书含,2017,香附浸提液对小麦种子的萌发及幼苗生长的影响,基因组学与应用生物学,36(9):3907-3911)
Wu Y.,Hu H.Q.,Wang G.,Zhang Y.Z.,and Ji J.,2002,Relationship between a-amylase activity and resistance of pre-harvest sprouting in spring wheat,Jinlin Nongye Daxue Xuebao(Journal of Jilin Agricultural University),24(4):22-25(吴颖,胡汉桥,王罡,张艳贞,季静,2002,春小麦a-淀粉酶活性及其与穗发芽抗性的关系,吉林农业大学学报,24(4):22-25)
Xu X.T.,Deng Z.P.,Zhong H.,and Yao Q.Q.,2012,Research progress on chemical constituents and pharmacological activities of Cyperus rotundus L.,Qilu Yaoshi(Qilu Pharmaceutical Affairs),31(8):473-475(徐晓婷,邓志鹏,仲浩,姚庆强,2012,香附化学成分及药理作用研究进展,齐鲁药事,31(8):473-475)
Xu Y.,Li D.X.,Ling T.J.,and Xiang W.,2010a,Advances in studies on chemical constitutents of rhizomes of Cyperus rotuudus,Zhongguo Shiyan Fangjixue Zazhi(China Journal of Experimental Traditional Medical Formulae),16(11):214-218(徐燕,李大祥,凌铁军,项威,2010a,香附化学成分研究进展,中国实验方剂学杂志,16(11):214-218)
Xu Y.,Zhang Z.Z.,and Zou Z.M.,2010b,Studies on chemical constituents of the rhizomes of Cyperus rotundus L.,Zhongguo Yaoxue Zazhi(China Pharmaceutical Journal),45(11):818-821(徐燕,张正竹,邹忠梅,2010b,香附的化学成分研究,中国药学杂志,45(11):818-821)
Xue X.,Wang Q.L.,and Ru P.P.,2007,Inhibiting effect of willow extracting solution on wheat sprouting,Zhongzi(Seed),26(3):38-40(薛香,王巧玲,茹萍萍,2007,柳枝浸提液对小麦籽粒发芽的抑制作用研究,种子,26(3):38-40)
Zhang H.F.,and Lu R.H.,1993,Study on mechanism of the resistance to preharvest sproutingand inberitance in wheat,Zuowu Xuebao(Acta Agronomica Sinica),19(6):523-529(张海峰,卢荣禾,1993,小麦穗发芽抗性机理与遗传研究,作物学报,19(6):523-530)
Zhang Q.J.,Yang X.S.,and Hao X.J.,2008,Advances in studies on new natural antibacterial compounds in China,Zhongcaoyao(Chinese Traditional and Herbal Drugs),39(2):304-308(张前军,杨晓生,郝小江,2008,我国天然抗菌药物研究进展,中草药,39(2):304-308)
Zhang Z.Y.,Tang J.X.,Han J.,and Shi X.R.,2009,Allellopathy of extracts of different cucumber(Cucumis sativus)varieties on Lettuce(lactuca sativa)seed germination,Hubei Nongye Kexue(Hubei Agricultural Sciences),47(11):1298-1300(张志勇,汤菊香,韩静,时小瑞,2009,不同黄瓜品种对莴苣种子萌发化感作用的影响,湖北农业科学,47(11):1298-1300)
Zheng X.F.,and Li C.X.,2008,Effects of Datura stramonium L's alkaloids on maize seeds germination and seedling growth,Xinan Shifan Daxue Xuebao(Journal of Southwest China Normal University(Natural Science)),33(5):121-125(郑秀芳,李彩霞,2008,曼陀罗生物碱对玉米种子萌发和幼苗生长的影响,西南师范大学学报,33(5):121-125)
Liu J.X.,Hu H.B.,Zhao G.L.,and Wang X.,2006,Effect of alkaloid extract from Peganum multisectum bobr on seed germination and seedling growth of wheat(Triticum aestivum L.),Zhiwu Shenglixue Tongxun(Plant Physiology Communications),42(2):213-216(刘建新,胡浩斌,赵国林,王鑫,2006,多裂骆驼蓬中生物碱类物质对小麦种子萌发和幼苗生长的影响,植物生理学通讯,42(2):213-216)
Lin X.S.,Shi H.Q.,Huang F.,and Huang X.L.,2006,Analysis of essential oil from extraction Cyperus rotundus L.by GC/MS,Zhipu Xuebao(Journal of Chinese Mass Spectrometry Society),27(1):40-44(林晓珊,吴惠勤,黄芳,黄晓兰,2006,香附挥发油的提取和GC/MS分析,质谱学报,27(1):40-44)
Sun G.Z.,Yan C.S.,and Xiao S.H.,2003,The mechanism on wheat pre-harvest sprouting,Zhongguo Nongye Kexue Daobao(Journal of Agricultural Science and Technology),5(6):13-18(孙果忠,闰长生,肖世和,2003,小麦穗发芽机制研究进展,中国农业科技导报,5(6):13-18)
Sun J.Y.,Li S.M.,Tong S.L.,and Liu S.H.,2017,Effect of leach liquor from Rhizoma cyperi on wheat seed germination and seedling growth,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),36(9):3907-3911(孙君艳,李淑梅,仝胜利,刘书含,2017,香附浸提液对小麦种子的萌发及幼苗生长的影响,基因组学与应用生物学,36(9):3907-3911)
Wu Y.,Hu H.Q.,Wang G.,Zhang Y.Z.,and Ji J.,2002,Relationship between a-amylase activity and resistance of pre-harvest sprouting in spring wheat,Jinlin Nongye Daxue Xuebao(Journal of Jilin Agricultural University),24(4):22-25(吴颖,胡汉桥,王罡,张艳贞,季静,2002,春小麦a-淀粉酶活性及其与穗发芽抗性的关系,吉林农业大学学报,24(4):22-25)
Xu X.T.,Deng Z.P.,Zhong H.,and Yao Q.Q.,2012,Research progress on chemical constituents and pharmacological activities of Cyperus rotundus L.,Qilu Yaoshi(Qilu Pharmaceutical Affairs),31(8):473-475(徐晓婷,邓志鹏,仲浩,姚庆强,2012,香附化学成分及药理作用研究进展,齐鲁药事,31(8):473-475)
Xu Y.,Li D.X.,Ling T.J.,and Xiang W.,2010a,Advances in studies on chemical constitutents of rhizomes of Cyperus rotuudus,Zhongguo Shiyan Fangjixue Zazhi(China Journal of Experimental Traditional Medical Formulae),16(11):214-218(徐燕,李大祥,凌铁军,项威,2010a,香附化学成分研究进展,中国实验方剂学杂志,16(11):214-218)
Xu Y.,Zhang Z.Z.,and Zou Z.M.,2010b,Studies on chemical constituents of the rhizomes of Cyperus rotundus L.,Zhongguo Yaoxue Zazhi(China Pharmaceutical Journal),45(11):818-821(徐燕,张正竹,邹忠梅,2010b,香附的化学成分研究,中国药学杂志,45(11):818-821)
Xue X.,Wang Q.L.,and Ru P.P.,2007,Inhibiting effect of willow extracting solution on wheat sprouting,Zhongzi(Seed),26(3):38-40(薛香,王巧玲,茹萍萍,2007,柳枝浸提液对小麦籽粒发芽的抑制作用研究,种子,26(3):38-40)
Zhang H.F.,and Lu R.H.,1993,Study on mechanism of the resistance to preharvest sproutingand inberitance in wheat,Zuowu Xuebao(Acta Agronomica Sinica),19(6):523-529(张海峰,卢荣禾,1993,小麦穗发芽抗性机理与遗传研究,作物学报,19(6):523-530)
Zhang Q.J.,Yang X.S.,and Hao X.J.,2008,Advances in studies on new natural antibacterial compounds in China,Zhongcaoyao(Chinese Traditional and Herbal Drugs),39(2):304-308(张前军,杨晓生,郝小江,2008,我国天然抗菌药物研究进展,中草药,39(2):304-308)
Zhang Z.Y.,Tang J.X.,Han J.,and Shi X.R.,2009,Allellopathy of extracts of different cucumber(Cucumis sativus)varieties on Lettuce(lactuca sativa)seed germination,Hubei Nongye Kexue(Hubei Agricultural Sciences),47(11):1298-1300(张志勇,汤菊香,韩静,时小瑞,2009,不同黄瓜品种对莴苣种子萌发化感作用的影响,湖北农业科学,47(11):1298-1300)
Zheng X.F.,and Li C.X.,2008,Effects of Datura stramonium L's alkaloids on maize seeds germination and seedling growth,Xinan Shifan Daxue Xuebao(Journal of Southwest China Normal University(Natural Science)),33(5):121-125(郑秀芳,李彩霞,2008,曼陀罗生物碱对玉米种子萌发和幼苗生长的影响,西南师范大学学报,33(5):121-125)