气相色谱内标法测定酸奶中木糖醇的含量
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摘要
目的优化毛细管气相色谱条件及前处理衍生过程并用于测定酸奶中木糖醇含量。方法对气相色谱分流比、进样量、程序升温等参数进行优化,并对前处理衍生过程进行筛选。在最优条件下,以内标法定量。结果在最优化条件下进行方法学验证,测得酸奶样品中木糖醇检出限为0.02%,定量限为0.068%。加标回收范围为91%～95%,相对标准偏差小于2%(n=6)。结论该方法简单易操作,色谱峰峰形好且稳定,可用于测定酸奶中木糖醇的含量。
Objective To optimize conditions of capillary gas chromatography and pretreatment derivative process for determination of xylitol content in yoghurt. Method The gas chromatographic split ratio, injection volume, temperature programming and other parameters were optimized, and the pretreatment derivative process was screened. Under the optimal conditions, the internal standard method was used to quantify the content. Results Under the optimization conditions, the limit of detection of xylitol in yoghurt samples was 0.02% and the limit of quantitation was 0.068%. The standard recovery range was 91% to 95%, and the relative standard deviation was less than 2%(n=6). Conclusion The method is simple and easy to operate, and the peaks of chromatography are good and stable, which is suitable for the determination of xylitol in yoghurt.
Objective To optimize conditions of capillary gas chromatography and pretreatment derivative process for determination of xylitol content in yoghurt. Method The gas chromatographic split ratio, injection volume, temperature programming and other parameters were optimized, and the pretreatment derivative process was screened. Under the optimal conditions, the internal standard method was used to quantify the content. Results Under the optimization conditions, the limit of detection of xylitol in yoghurt samples was 0.02% and the limit of quantitation was 0.068%. The standard recovery range was 91% to 95%, and the relative standard deviation was less than 2%(n=6). Conclusion The method is simple and easy to operate, and the peaks of chromatography are good and stable, which is suitable for the determination of xylitol in yoghurt.
引文
[1]GB 2760-2014食品安全国家标准食品添加剂使用标准[S].GB 2760-2014 Food safety national standard-Standard for the use of food additives[S].
[2]吕白凤.木糖醇在各类食品中的应用[J].农村新技术,2010,(16):23-24.Lv BF.The application of xylitol in various foods[J].New Rural Technol,2010,(16):23-24.
[3]李素琴,高晓娟,苏鑫.露酒中木糖醇检测方法的研究[J].酿酒,2019,46(1):101-103.Li SQ,Gao XJ,Su X.Study on the determination of xylitol in wine[J].Liquor Making,2019,46(1):101-103.
[4]方春雷,王世红,梁莉.木糖醇防治骨质疏松的功能研究概述[J].山东化工,2016,(45):50-52.Fang CL,Wang SH,Liang L.Xylitol functional research overview of osteoporosis prevention[J].Shandong Chem Ind,2016,(45):50-52.
[5]尤新.木糖醇作为食糖替代品的特性和应用[J].中国食品添加剂,2004,(2):1-5.You X.The characteristics and application of xylitol as a sugar substitute[J].Chin Food Addit,2004,(2):1-5.
[6]成妮妮,陈广艳.木糖醇石榴汁酸奶发酵工艺研究[J].食品研究与开发,2017,38(2):121-125.Cheng NN,Chen GY.Study on yoghurt fermentation technology of xylitol pomegranate juice[J].Food Res Dev,2017,38(2):121-125.
[7]赵亚许,张小芳,刘玉青.木糖醇南瓜酸奶的工艺研究[J].食品工程,2014,(2):23-25.Zhao YX,Zhang XF,Liu YQ.Study on the technology of xylitol pumpkin yogurt[J].Food Eng,2014,(2):23-25.
[8]王蕊.功能性甜味剂木糖醇及在食品加工中的应用[J].江苏食品与发酵,2008,(2):18-20.Wang R.Functional sweetener xylitol and application in food processing[J].Jiangsu Food Ferment,2008,(2):18-20.
[9]李疆,陈桐,鞠昭函,等.毛细管区带电泳-间接紫外测定食品中木糖醇[J].食品安全质量检测学报,2016,7(2):791-797.Li J,Chen T,Ju ZH,et al.Capillary district electric swimming-indirect ultraviolet determination of xylitol in food[J].J Food Saf Qual,2016,7(2):791-797.
[10]杨柳,赵聪,姚默,等.木糖醇应用及毒副作用研究进展[J].安徽农业科学,2011,39(34):21355-21356.Yang L,Zhao C,Yao M,et al.Application of xylitol and toxic side effects research progress[J].Anhui Agric Sci,2011,39(34):21355-21356.
[11]GB 5009.279-2016食品安全国家标准食品中木糖醇、山梨醇、麦芽糖醇、赤藓糖醇的测定[S].GB 5009.279-2016 Food safety national standard-Determination of xylitol,sorbitol,maltol,and albite in food[S].
[12]王成福,赵光辉,周娟.气相色谱毛细管柱对木糖醇产品的测定[J].中国食品添加剂,2005,(3):100-107.Wang CF,Zhao GH,Zhou J.Determination of xylitol products by gas chromatography capillary column[J].Chin Food Addit,2005,(3):100-107.
[13]曹元龙,刘红利,李永利.食品中木糖醇含量的气相色谱法测定[J].河南预防医学杂志,2000,11(5):260-261.Cao YL,Liu HL,Li YL.Determination of xylitol content in foods by gas chromatography[J].Henan J Prev Med,2000,11(5):260-261.
[14]GB 1886.234-2016食品安全国家标准食品添加剂木糖醇[S].GB 1886.234-2016 Food safety national standard-Food additive xylitol[S].
[15]中华人民共和国药典第四部[M].北京:中国医药科技出版社,2015.People's Republic of China Pharmacopoeia Part IV[M].Beijing:China Medical Science and Technology Press,2015.
[2]吕白凤.木糖醇在各类食品中的应用[J].农村新技术,2010,(16):23-24.Lv BF.The application of xylitol in various foods[J].New Rural Technol,2010,(16):23-24.
[3]李素琴,高晓娟,苏鑫.露酒中木糖醇检测方法的研究[J].酿酒,2019,46(1):101-103.Li SQ,Gao XJ,Su X.Study on the determination of xylitol in wine[J].Liquor Making,2019,46(1):101-103.
[4]方春雷,王世红,梁莉.木糖醇防治骨质疏松的功能研究概述[J].山东化工,2016,(45):50-52.Fang CL,Wang SH,Liang L.Xylitol functional research overview of osteoporosis prevention[J].Shandong Chem Ind,2016,(45):50-52.
[5]尤新.木糖醇作为食糖替代品的特性和应用[J].中国食品添加剂,2004,(2):1-5.You X.The characteristics and application of xylitol as a sugar substitute[J].Chin Food Addit,2004,(2):1-5.
[6]成妮妮,陈广艳.木糖醇石榴汁酸奶发酵工艺研究[J].食品研究与开发,2017,38(2):121-125.Cheng NN,Chen GY.Study on yoghurt fermentation technology of xylitol pomegranate juice[J].Food Res Dev,2017,38(2):121-125.
[7]赵亚许,张小芳,刘玉青.木糖醇南瓜酸奶的工艺研究[J].食品工程,2014,(2):23-25.Zhao YX,Zhang XF,Liu YQ.Study on the technology of xylitol pumpkin yogurt[J].Food Eng,2014,(2):23-25.
[8]王蕊.功能性甜味剂木糖醇及在食品加工中的应用[J].江苏食品与发酵,2008,(2):18-20.Wang R.Functional sweetener xylitol and application in food processing[J].Jiangsu Food Ferment,2008,(2):18-20.
[9]李疆,陈桐,鞠昭函,等.毛细管区带电泳-间接紫外测定食品中木糖醇[J].食品安全质量检测学报,2016,7(2):791-797.Li J,Chen T,Ju ZH,et al.Capillary district electric swimming-indirect ultraviolet determination of xylitol in food[J].J Food Saf Qual,2016,7(2):791-797.
[10]杨柳,赵聪,姚默,等.木糖醇应用及毒副作用研究进展[J].安徽农业科学,2011,39(34):21355-21356.Yang L,Zhao C,Yao M,et al.Application of xylitol and toxic side effects research progress[J].Anhui Agric Sci,2011,39(34):21355-21356.
[11]GB 5009.279-2016食品安全国家标准食品中木糖醇、山梨醇、麦芽糖醇、赤藓糖醇的测定[S].GB 5009.279-2016 Food safety national standard-Determination of xylitol,sorbitol,maltol,and albite in food[S].
[12]王成福,赵光辉,周娟.气相色谱毛细管柱对木糖醇产品的测定[J].中国食品添加剂,2005,(3):100-107.Wang CF,Zhao GH,Zhou J.Determination of xylitol products by gas chromatography capillary column[J].Chin Food Addit,2005,(3):100-107.
[13]曹元龙,刘红利,李永利.食品中木糖醇含量的气相色谱法测定[J].河南预防医学杂志,2000,11(5):260-261.Cao YL,Liu HL,Li YL.Determination of xylitol content in foods by gas chromatography[J].Henan J Prev Med,2000,11(5):260-261.
[14]GB 1886.234-2016食品安全国家标准食品添加剂木糖醇[S].GB 1886.234-2016 Food safety national standard-Food additive xylitol[S].
[15]中华人民共和国药典第四部[M].北京:中国医药科技出版社,2015.People's Republic of China Pharmacopoeia Part IV[M].Beijing:China Medical Science and Technology Press,2015.