杜马斯燃烧法快速检测液体乳制品中蛋白质
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摘要
采用杜马斯燃烧法建立液体乳制品中蛋白质的快速检测方法。选取37种液体乳制品,分别采用凯氏定氮法和杜马斯燃烧法进行蛋白质检测,对比结果表明2种方法精密度之间并无显著性差异,但在方法上存在一定的差异。精密度、准确度和加标回收率实验结果表明杜马斯燃烧法的标准差小于2%;以凯氏定氮法结果为标准值,杜马斯燃烧法结果的相对偏差小于5%;加标回收率结果范围为99.0%~101.3%,说明杜马斯燃烧法检测液体乳制品仍然满足标准检测要求,用来检测液体乳制品中蛋白质是可行的。
Built a rapid detection method by Dumas combustion method to protein in liquid dairy products. Selected 37 kinds of liquid milk products, using Kjeldahl method and Dumas combustion method respectively for protein detection, the results showed that the two methods' precision had no significant difference, but there were some differences in the method. The precision, accuracy and recovery experiment results showed that the standard deviation of Dumas combustion method was less than 2%; the relative deviation of the results of Dumas combustion method was less than 5% by referencing the Kjeldahl method results as the standard value; the range of the recovery rate was about 99.0%~101.3%. These could testify that Dumas combustion method could meet the requirements of standard detection, and it was feasible of detection of protein in liquid dairy products by Dumas combustion method.
Built a rapid detection method by Dumas combustion method to protein in liquid dairy products. Selected 37 kinds of liquid milk products, using Kjeldahl method and Dumas combustion method respectively for protein detection, the results showed that the two methods' precision had no significant difference, but there were some differences in the method. The precision, accuracy and recovery experiment results showed that the standard deviation of Dumas combustion method was less than 2%; the relative deviation of the results of Dumas combustion method was less than 5% by referencing the Kjeldahl method results as the standard value; the range of the recovery rate was about 99.0%~101.3%. These could testify that Dumas combustion method could meet the requirements of standard detection, and it was feasible of detection of protein in liquid dairy products by Dumas combustion method.
引文
[1]苏海霞.乳制品的分类与营养[J].食品安全导刊,2016,(9):55-56
[2]侯向昶,董浩,罗东辉,等.乳与乳制品中蛋白质检测方法的研究现状与进展[J].乳业科学与技术,2013,36(3):28-31
[3]宋思远,李巧玲,刘英华,等.乳及乳制品中蛋白质检测技术的研究进展[J].江苏农业科学,2012,40(5):279-282
[4]王钦权,翁佳妍,黄诚,等.凯氏定氮法和杜马斯燃烧法测定食品中蛋白质含量的比较研究[J].轻工科技,2014,(3):13-14
[5]Thierry Saint-Denis,Jacques Goupy.Optimization of a nitrogen analyser based on the Dumas method[J].Analytica Chimica Acta 2004,515:191-198
[6]韩博,金凯,张文娟,等.凯氏定氮法与杜马斯燃烧法测定大豆粗蛋白的比较研究[J].中国畜牧杂志,2010,46(23):67-69
[7]Etheridge R D,Pesti G M,Foster E H.A comparison of nitrogen values obtained utilizing the Kjeldahl nitrogen and Dumas combustion methodologies(LecoCNS-2000)on samples typical of an animal nutrition analytical laboratory[J].Animal Feed Science and Technology,1998,73:21-28
[8]Michael Thompson,Linda Owen,Kate Wilkinson.Testing for bias between the Kjeldahl and Dumas methods for the determination of nitrogen in meat mixtures by using data from a designed interlaboratory experiment[J].Meat Science,2004,68:631-634
[9]Jung S,Rickert DA,Deak NA.Comparison of Kjeldahl and Dumas methods for determining protein contents of soybean products[J].Journal of the American Oil Chemists Society,2003,80(12):1169-1173
[10]Chiacchierini E,Ascenzo F,Restuccia D.Milk soluble whey proteins:Fast and precise determination with Dumas method[J].Analytical Letters,2003,36(11):2473-2484
[11]徐丽,翟丽娜,刘亚楠,等.杜马斯燃烧法测定牛乳中的蛋白质含量[J].食品安全质量检测学报,2014,12(5):3903-3905
[12]郭望山,孟庆翔.杜马斯燃烧法与凯氏法测定饲料含氮量的比较研究[J].畜牧兽医报,2006,37(5):464-468
[13]Waston ME,Galliber TL.Comparison of Dumas and Kjeldahl method with automatic analyzers on agricultural samples under routine rapid analysis conditions[J].Commum Soil Sci Plant Ana,2001,32:2007-2019
[14]陈涛,曾炫萍,宋悦华,等.乳制品中硝酸盐和亚硝酸盐检验方法的改进[J].中国卫生检验杂志,2010,20(7):1807-1810
[15]赵佳,董永,马晓杰,等.我国29省市自治区市售纯牛乳亚硝酸盐含量普查[J].中国奶牛,2011,(12):55-59
[2]侯向昶,董浩,罗东辉,等.乳与乳制品中蛋白质检测方法的研究现状与进展[J].乳业科学与技术,2013,36(3):28-31
[3]宋思远,李巧玲,刘英华,等.乳及乳制品中蛋白质检测技术的研究进展[J].江苏农业科学,2012,40(5):279-282
[4]王钦权,翁佳妍,黄诚,等.凯氏定氮法和杜马斯燃烧法测定食品中蛋白质含量的比较研究[J].轻工科技,2014,(3):13-14
[5]Thierry Saint-Denis,Jacques Goupy.Optimization of a nitrogen analyser based on the Dumas method[J].Analytica Chimica Acta 2004,515:191-198
[6]韩博,金凯,张文娟,等.凯氏定氮法与杜马斯燃烧法测定大豆粗蛋白的比较研究[J].中国畜牧杂志,2010,46(23):67-69
[7]Etheridge R D,Pesti G M,Foster E H.A comparison of nitrogen values obtained utilizing the Kjeldahl nitrogen and Dumas combustion methodologies(LecoCNS-2000)on samples typical of an animal nutrition analytical laboratory[J].Animal Feed Science and Technology,1998,73:21-28
[8]Michael Thompson,Linda Owen,Kate Wilkinson.Testing for bias between the Kjeldahl and Dumas methods for the determination of nitrogen in meat mixtures by using data from a designed interlaboratory experiment[J].Meat Science,2004,68:631-634
[9]Jung S,Rickert DA,Deak NA.Comparison of Kjeldahl and Dumas methods for determining protein contents of soybean products[J].Journal of the American Oil Chemists Society,2003,80(12):1169-1173
[10]Chiacchierini E,Ascenzo F,Restuccia D.Milk soluble whey proteins:Fast and precise determination with Dumas method[J].Analytical Letters,2003,36(11):2473-2484
[11]徐丽,翟丽娜,刘亚楠,等.杜马斯燃烧法测定牛乳中的蛋白质含量[J].食品安全质量检测学报,2014,12(5):3903-3905
[12]郭望山,孟庆翔.杜马斯燃烧法与凯氏法测定饲料含氮量的比较研究[J].畜牧兽医报,2006,37(5):464-468
[13]Waston ME,Galliber TL.Comparison of Dumas and Kjeldahl method with automatic analyzers on agricultural samples under routine rapid analysis conditions[J].Commum Soil Sci Plant Ana,2001,32:2007-2019
[14]陈涛,曾炫萍,宋悦华,等.乳制品中硝酸盐和亚硝酸盐检验方法的改进[J].中国卫生检验杂志,2010,20(7):1807-1810
[15]赵佳,董永,马晓杰,等.我国29省市自治区市售纯牛乳亚硝酸盐含量普查[J].中国奶牛,2011,(12):55-59