无毛囊发干遗传分析新标记的应用
|
摘要
目的解决法医检验工作中自然脱落的毛发、严重降解的骨骼及牙齿这类检材检验难度大且检出完整DNA分型的成功率低的问题。方法应用一类特殊的DNA序列——逆转座子(retrotransponson,RE)进行检验鉴定,用作遗传分析的RE DNA片段长度多在60~125 bp,利用21个基因座组合的RE个体识别系统计算似然比(likelihood,LR),并计算亲缘关系鉴定中的累计非父排除率(combined paternity index,CPI)。结果本系统对无毛囊的发干、严重降解的生物检材有较高的检出成功率,LR值多在10~8~10~(12),完全能够达到同一认定的水平。计算CPI值后发现难以得出支持假设的结论,多个案例计算的CPI数值小于10 000。结论 21个基因座的RE系统可以用于个体识别鉴定,亲缘关系的鉴定仍需增加基因座。
Objective To solve the problems of low success rate in detecting complete DNA typing of biological samples,such as naturally fallen hair,severely degraded bones and teeth which is difficult in forensic identification. Methods We applyed a specific retrotransposon(RE)DNA sequences in identification,with DNA fragment length of 60-125 bp.A RE combined with 21 loci were used in individual discrimination to compute the likelihood(LR)values and combined paternity index(CPI)values in phylogenetic identification. Results This research obtained high success rate in detection of hair stem without follicles and severely degraded biological samples.We found that most of the LR values were 10~8-10~(12),which met the requirement of identity establishing.Through the CPI values,it was difficult to support the assumptions,with most of the CPI values less than 10 000. Conclusions The RE with 21 loci can be used for individual discrimination,but more loci are need for applying in phylogenetic identification.
Objective To solve the problems of low success rate in detecting complete DNA typing of biological samples,such as naturally fallen hair,severely degraded bones and teeth which is difficult in forensic identification. Methods We applyed a specific retrotransposon(RE)DNA sequences in identification,with DNA fragment length of 60-125 bp.A RE combined with 21 loci were used in individual discrimination to compute the likelihood(LR)values and combined paternity index(CPI)values in phylogenetic identification. Results This research obtained high success rate in detection of hair stem without follicles and severely degraded biological samples.We found that most of the LR values were 10~8-10~(12),which met the requirement of identity establishing.Through the CPI values,it was difficult to support the assumptions,with most of the CPI values less than 10 000. Conclusions The RE with 21 loci can be used for individual discrimination,but more loci are need for applying in phylogenetic identification.
引文
[1]刘开会,郭燕霞,季安全.毛干及自然脱落毛囊复合扩增Amel及13个STR基因座检验的实验研究[J].中国法医学杂志,2003,18(5):294-296.
[2]程宝文,刘永剑,李文富.毛干mtDNA单倍型母系家系排查法侦破命案[J].中国法医学杂志,2010,25(1):48-49.
[3]ISKOW RC,MCCABE MT,MILLS RE,et al.Natural mutagenesis of human genomes by endogenous retrotransposons[J].Cell,2010,141(7):1253-1261.
[4]WAGSTAFF BJ,BAMERSSOI M,ROYAL-ANGEL AM,Evolutionary conservation of the functional modularity of primate and murine line-1 elements[J].PLoS ONE,2011,6(5):e19672.
[5]SMIT AF.Interspersed repeats and other mementos of transposable elements in mammalian genomes[J]Curr Opin Genet Dev,1999,9(6):657-663.
[6]凃政,陈松,李万水.脱落毛发及毛干DNA的STR分型研究[J].刑事技术,2011(5):3-7.
[7]王林生,苏勇,顾林岗.硅珠法提取PCR模板DNA[J].中国法医学杂志,2000,15(1):36-37.
[8]ECKHART L,BACH J,BAN J,et al.Melanin binds reversibly to thermostable DNA polymerase and inhibits its activity[J].Biochem Biophys Res Commun,2000,271(3):726-730.
[9]ANNILAURI VILLEME BS,GRETCHEN BARTIZAL MS,CAROLYN RS,et al.Examination of 20retrotransposable polymorphic insertion/Null(IN/NUL)markers for their utility in kinship testing using a commercial software program[R].Las Vegas:AAFS Annual Meeting NV,2016.
[10]PETERS D,GRISEDALE K,SCHADE L,et al.The width of a human hair,how new technologies are getting result from challenging DNA samples?[J].Forensic Mag,2015,9:22-24.
[2]程宝文,刘永剑,李文富.毛干mtDNA单倍型母系家系排查法侦破命案[J].中国法医学杂志,2010,25(1):48-49.
[3]ISKOW RC,MCCABE MT,MILLS RE,et al.Natural mutagenesis of human genomes by endogenous retrotransposons[J].Cell,2010,141(7):1253-1261.
[4]WAGSTAFF BJ,BAMERSSOI M,ROYAL-ANGEL AM,Evolutionary conservation of the functional modularity of primate and murine line-1 elements[J].PLoS ONE,2011,6(5):e19672.
[5]SMIT AF.Interspersed repeats and other mementos of transposable elements in mammalian genomes[J]Curr Opin Genet Dev,1999,9(6):657-663.
[6]凃政,陈松,李万水.脱落毛发及毛干DNA的STR分型研究[J].刑事技术,2011(5):3-7.
[7]王林生,苏勇,顾林岗.硅珠法提取PCR模板DNA[J].中国法医学杂志,2000,15(1):36-37.
[8]ECKHART L,BACH J,BAN J,et al.Melanin binds reversibly to thermostable DNA polymerase and inhibits its activity[J].Biochem Biophys Res Commun,2000,271(3):726-730.
[9]ANNILAURI VILLEME BS,GRETCHEN BARTIZAL MS,CAROLYN RS,et al.Examination of 20retrotransposable polymorphic insertion/Null(IN/NUL)markers for their utility in kinship testing using a commercial software program[R].Las Vegas:AAFS Annual Meeting NV,2016.
[10]PETERS D,GRISEDALE K,SCHADE L,et al.The width of a human hair,how new technologies are getting result from challenging DNA samples?[J].Forensic Mag,2015,9:22-24.