一种焚烧骨骼的DNA提取方法
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摘要
本文介绍了一种针对烧骨的DNA有效提取方法。对烧骨进行研磨制成骨粉,对骨粉进行大体系脱钙和裂解,采用Amicon~Ultra-15离心过滤器对获得的脱钙、裂解上清液进行浓缩,提高DNA的回收率,使用硅胶膜法对浓缩后的DNA溶液进行纯化,并经复合STR扩增检验。使用上述方法成功完成两起严重焚烧骨骼案件的检验,确定了受害人身源。
This paper introduces a new DNA extraction approach for burned skeletal remains. The burned bones were grinded into powder, and then decalcified and digested in a large volume. Amicon~ Ultra-15 centrifugal filter device was adopted to concentrate the supernatant after decalcification and digestion in order to increase the DNA recovery efficiency. Then the concentrated DNA supernatant was purified using silica gel membrane method. Autosomal STR genotyping was performed with commercial STR typing kits. Using this method, we have successfully identified the severely burned bones in two real cases, providing the key evidences for solving these cases.
This paper introduces a new DNA extraction approach for burned skeletal remains. The burned bones were grinded into powder, and then decalcified and digested in a large volume. Amicon~ Ultra-15 centrifugal filter device was adopted to concentrate the supernatant after decalcification and digestion in order to increase the DNA recovery efficiency. Then the concentrated DNA supernatant was purified using silica gel membrane method. Autosomal STR genotyping was performed with commercial STR typing kits. Using this method, we have successfully identified the severely burned bones in two real cases, providing the key evidences for solving these cases.
引文
[1]YE J,JI AQ,Esteban J,et al.A simple and efficient method for extracting DNA from old and burned bone.J Forensic Sci,2004,49:754-759.
[2]凃政,刘志芳,陈松,等.牙齿的DNA提取及STR分型研究.刑事技术,2007(5):9-10.
[3]Cynthia R,Ayda LM,Emilio JY,et al.Comparison of two methods in isolating DNA from human skeleton for STR analysis.J Forensic Sci,2012,57(3):706-712.
[4]李花,凃政,石屹,等.骨骼脱钙后DNA含量损失问题初探.中国法医学杂志,2014,29(2):141-144.
[5]徐国昌,侯续伟,任甫,等.不同温度焚烧对成人股骨16个基因位点的影响.解剖学杂志,2008,31(3):406-408.
[6]Thompson TJ.Recent advances in the study of burned bone and their implications for forensic anthropology.J Forensic Sci Int,2004,146(l):203-205.
[7]张继宗,王北瑜.炭化尸体的法医学鉴定.刑事技术,2000(2):36-37.
[8]Holden JL,Phakey PP,Clement JG.Scanning electron microscope observations of heat-treated human bone.J Forensic Sci Int,1995,74(2):29-45.
[9]徐国昌,张培华,任甫,等.基于光学、电子显微镜图像的烧骨组织学变化特征.解剖学杂志,2012,35(3):380-383.
[10]刘海东,任甫,席焕久,等.个体识别中烧骨的DNA提取.解剖学杂志,2010,33(1):119-121.
[2]凃政,刘志芳,陈松,等.牙齿的DNA提取及STR分型研究.刑事技术,2007(5):9-10.
[3]Cynthia R,Ayda LM,Emilio JY,et al.Comparison of two methods in isolating DNA from human skeleton for STR analysis.J Forensic Sci,2012,57(3):706-712.
[4]李花,凃政,石屹,等.骨骼脱钙后DNA含量损失问题初探.中国法医学杂志,2014,29(2):141-144.
[5]徐国昌,侯续伟,任甫,等.不同温度焚烧对成人股骨16个基因位点的影响.解剖学杂志,2008,31(3):406-408.
[6]Thompson TJ.Recent advances in the study of burned bone and their implications for forensic anthropology.J Forensic Sci Int,2004,146(l):203-205.
[7]张继宗,王北瑜.炭化尸体的法医学鉴定.刑事技术,2000(2):36-37.
[8]Holden JL,Phakey PP,Clement JG.Scanning electron microscope observations of heat-treated human bone.J Forensic Sci Int,1995,74(2):29-45.
[9]徐国昌,张培华,任甫,等.基于光学、电子显微镜图像的烧骨组织学变化特征.解剖学杂志,2012,35(3):380-383.
[10]刘海东,任甫,席焕久,等.个体识别中烧骨的DNA提取.解剖学杂志,2010,33(1):119-121.