3个藏药经典方剂治疗脑缺血有效物质研究
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摘要
目的建立可用于筛选脑缺血药效的细胞模型,并从3个治疗脑缺血的经典藏药方剂中寻找其发挥不同药效的物质基础。方法以HEK-293细胞为对象,转染含有血管内皮生长因子(VEGF)基因启动子的质粒,构建细胞模型,并验证;用得到的细胞模型进行筛选3个藏药中的主要成分。结果阳性药物可有效上调该细胞模型的荧光素酶活性;黄芪甲苷、丁香酚、没食子酸、芫花素可上调VEGF启动子水平至150%以上,阿魏酸、川芎嗪、芒果苷可上调VEGF启动子水平至20倍以上。结论该细胞模型可响应阳性药物的刺激,可靠性佳;推测阿魏酸、川芎嗪、芒果苷3个成分在3个藏药经典方剂的血管再生药效中起主要作用。
Objective To establish a cell model for screening the pharmacodynamics of cerebral ischemia and to find out the material basis of different pharmacodynamics from three classical Tibetan medicine prescriptions for treating cerebral ischemia.Methods The plasmid containing vascular endothelial growth factor(VEGF) gene promoter were transfected into the HEK-293 cell to build the cell model and validate it.The cell model was used to screen the main components of three Tibetan medicine prescriptions.Results Positive drugs could effectively up-regulate the luciferase activity of the cell model.Astragaloside Ⅳ,eugenol,gallic acid and genkwanin could up-regulate the level of VEGF promoter to more than 150%.Ferulic acid,tetramethylpyrazine and mangiferin could up-regulate the level of VEGF promoter to more than 20 times.Conclusion The cell model can respond to the stimulation of positive drugs and has good reliability.It is speculated that ferulic acid,tetramethylpyrazine and mangiferin in the three classical Tibetan medicine prescriptions play an important role in the regeneration of blood vessels.
Objective To establish a cell model for screening the pharmacodynamics of cerebral ischemia and to find out the material basis of different pharmacodynamics from three classical Tibetan medicine prescriptions for treating cerebral ischemia.Methods The plasmid containing vascular endothelial growth factor(VEGF) gene promoter were transfected into the HEK-293 cell to build the cell model and validate it.The cell model was used to screen the main components of three Tibetan medicine prescriptions.Results Positive drugs could effectively up-regulate the luciferase activity of the cell model.Astragaloside Ⅳ,eugenol,gallic acid and genkwanin could up-regulate the level of VEGF promoter to more than 150%.Ferulic acid,tetramethylpyrazine and mangiferin could up-regulate the level of VEGF promoter to more than 20 times.Conclusion The cell model can respond to the stimulation of positive drugs and has good reliability.It is speculated that ferulic acid,tetramethylpyrazine and mangiferin in the three classical Tibetan medicine prescriptions play an important role in the regeneration of blood vessels.
引文
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[2]孙绪丁,任松鹏,刘玉芹.如意珍宝丸质量标准研究[J].西部中医药,2013,26(1):24-30.
[3]刘震东,宋淼,达番琼,等.藏药二十味沉香丸的质量标准研究[J].华西药学杂志,2003,18(6):462-464.
[4]应旭辉.藏药二十五味珊瑚丸化学成分分析及神经保护作用物质基础研究[D].天津:南开大学,2014.
[5]SENGER DR,GALLI SJ,DVORAK AM,et al.Tumor cells secrete a vascular permeability factor that promotes accumulation of ascites fluid[J].Science,1983,219(4587):983-985.
[6]KAWAMURA H,LI X,GOISHI K,et al.Neuropilin-1 in regulation of VEGF-induced activation of p38MAPK and endothelial cell organization[J].Blood,2008,112(9):3638-3649.
[7]刘中原,李延平.细菌脂多糖的生物活性及作用机制[J].医学综述,2010,16(2):166-169.
[8]朋毛端知.萨热十三味鹏鸟丸治疗外白脉病[J].中国民族医药杂志,2014,20(2):56.
[9]FERRARA N.Vascular endothelial growth factor:Basic science andclinicalprogress[J].EndocrineReviews,2003,25(4):581-611.
[10]NICO B,MANGIERI D,BENAGIANO V,et al.Nerve growth factor as an angiogenic factor[J].Microvascular Research,2008,75(2):135-141.
[11]GOLDBERG MA,SCHNEIDER TJ.Similarities between the oxygen-sensing mechanisms regulating the expression of vascular endothelial growth factor and erythropoietin[J].J Biol Chem,1994,269(6):4355-4361.