Q热诊断技术研究进展
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摘要
Q热是由贝氏柯克斯体引起的一种自然疫源性人兽共患病。本文论述了Q热诊断中常见的血清学和病原学诊断技术,包括补体结合试验(CFT)、酶联免疫吸附试验(ELISA)、间接免疫荧光法(IFT)、细胞培养、PCR技术、环介导等温扩增(LAMP)、重组酶聚合酶扩增(RPA)和基因芯片等,并对各种技术优缺点进行比较分析,以期为该病的诊断与防控提供参考。
Q fever is a kind of natural-focal zoonosis caused by Coxiella burnetii. Here,the serological and etiological techniques commonly used in detection of Q fever diagnosis were reviewed,including complement ?xation test(CFT),enzyme-linked immunosorbent assay(ELISA),indirect immunofluorescence assay(IFA),cell culture,PCR,loop-mediated isothermal ampli?cation(LAMP),recombinase polymerase ampli?cation(RPA)and gene microarray. In addition,the advantages and disadvantages of the above techniques were compared and analyzed,hoping to provide reference for the diagnosis and control of the disease.
Q fever is a kind of natural-focal zoonosis caused by Coxiella burnetii. Here,the serological and etiological techniques commonly used in detection of Q fever diagnosis were reviewed,including complement ?xation test(CFT),enzyme-linked immunosorbent assay(ELISA),indirect immunofluorescence assay(IFA),cell culture,PCR,loop-mediated isothermal ampli?cation(LAMP),recombinase polymerase ampli?cation(RPA)and gene microarray. In addition,the advantages and disadvantages of the above techniques were compared and analyzed,hoping to provide reference for the diagnosis and control of the disease.
引文
[1]DE LANGE M M,SCHIMMER B,VELLEMA P,et al.Coxiella burnetii seroprevalence and risk factors in sheep farmers and farm residents in the Netherlands[J].Epidemiology and infection,2014,142(6):1231-1244.
[2]MEGE J L,MAURIN M,CAPO C,et al.Coxiella burnetii:the‘query’fever bacterium.a model of immune subversion by a strictly intracellular microorganism[J].Fems microbiol review,1997,19(4):209-217.
[3]张丽娟.中国立克次体病监测及防治现状与展望[J].疾病监测,2007,22(9):577-579.
[4]KHALILI M,SHAHABINEJAD N,GOLCHIN M.Q fever serology in febrile patients in southeast Iran[J].Transactions of the royal society of tropical medicine&hygiene,2010,104(9):623-624.
[5]SLAVIN G.‘Q’fever:the domestic animal as a source of infection for man[J].Veterinary record,1952,64:743-753.
[6]BENENSON A S,TIGERTT W D.Studies on Q fever in man[J].Transactions of the association of American physicians,1956,69:98-104.
[7]SIDI-BOUMEDINE K,ROUSSET E,HENNING K,et al.Development of harmonised schemes for the monitoring and reporting of Q-fever in animals in the European Union[J].EFSA scientific report,2010:1-48.
[8]SZYMA?SKA-CZERWI?SKA M,GALI?SKA E M,NIEMCZUK K,et al.Prevalence of Coxiella burnetii infection in humans occupationally exposed to animals in Poland[J].Vector-borne zoonotic diseases,2015,15(4):261-267.
[9]SZYMA?SKA-CZERWI?SKA M,GALI?SKA E M,NIEMCZUK K,et al.Prevalence of Coxiella burnetii infection in foresters and ticks in the south-eastern Poland and comparison of diagnostic methods[J].Annals of agricultural and environmental medicine,2013,20(4):699-704.
[10]武文君,贾广乐,廖娟红,等.Q热贝氏柯克斯体间接ELISA检测方法的建立[J].中国畜牧兽医,2014,41(4):95-101.
[11]KLEMMER J,NJERU J,EMAM A,et al.Q fever in Egypt:Epidemiological survey of Coxiella burnetii specific antibodies in cattle,buffaloes,sheep,goats and camels[J].Plos one.2018,13(2):e0192188.
[12]孙长俭,周连庆,薛文成,等.辽宁省农村地区Q热立克次体血清学及虫媒蜱携带病原体状况调查[J].华南国防医学杂志,2016,30(6):362-364.
[13]W E G D A M B L A N S M C,W I E L D E R S C C,MEEKELENKAMP J,et al.Evaluation of commonly used serological tests for detection of Coxiella burnetii antibodies in well-defined acute and follow-up sera[J].Clinical&vaccine immunology cvi,2012,19(7):1110-1115.
[14]OMSLAND A,COCKRELL D C,HOWE D,et al.Host cell-free growth of the Q fever bacterium Coxiella burnetii[J].Proceedings of the national academy of sciences of the United States of America.2009,106(11):4430-4434.
[15]MARES-GUIA,MARIA ANGéLIA M M,GUTERRES A,et al.Clinical and epidemiological use of nested PCRtargeting the repetitive element IS1111 associated with the transposase gene from Coxiella burnetii[J].Brazilian journal of microbiology,2018,49(1):138-143.
[16]亚红祥,张丽娟,张云智.Real-time PCR与常规PCR在Q热立克体检测中的比较[J].中国热带医学,2014,14(9):1054-1056.
[17]张琪,徐睿瑶,胡梦佳,等.贝氏柯克斯体可视化等温扩增法建立[J].中国公共卫生,2017,33(8):1219-1223.
[18]CHEN H W,CHING W M.Evaluation of the stability of lyophilized loop-mediated isothermal amplification reagents for the detection of Coxiella burnetii[J].Heliyon,2017,3(10):e00415.
[19]樊晓旭,赵永刚,李林,等.重组酶聚合酶扩增技术在疾病快速检测中的研究进展[J].中国动物检疫,2016,33(8):72-77.
[20]QI Y,YIN Q,SHAO Y,et al.Rapid and visual detection of Coxiella burnetii using recombinase polymerase amplification combined with lateral flow strips[J].Biomed research international,2018:6417354.
[21]KOO B,JIN C E,PARK S Y,et al.A rapid bio-optical sensor for diagnosing Q fever in clinical specimens[J].Biophotonics.2018,11:e201700167.
[22]MCLOUGHLIN K S.Microarrays for pathogen detection and analysis[J].Briefings in functional genomics,2011,10(6):342-353.
[23]焦俊.贝氏柯克斯体表面蛋白和T细胞表位的鉴定及免疫原性研究[D].北京:中国人民解放军军事医学科学院,2017.
[24]SCHMOOCK G,EHRICHT R,SPRAGUE L D.DNA microarray-based detection of Coxiella burnetii,the causative agent of Q fever[J].Acta veterinaria scandinavica,2014,56(1ya):27.
[25]王涛,宋立华.贝氏柯克斯体的分子致病机理研究进展[J].中国人兽共患病学报,2015,31(9):876-880.
[2]MEGE J L,MAURIN M,CAPO C,et al.Coxiella burnetii:the‘query’fever bacterium.a model of immune subversion by a strictly intracellular microorganism[J].Fems microbiol review,1997,19(4):209-217.
[3]张丽娟.中国立克次体病监测及防治现状与展望[J].疾病监测,2007,22(9):577-579.
[4]KHALILI M,SHAHABINEJAD N,GOLCHIN M.Q fever serology in febrile patients in southeast Iran[J].Transactions of the royal society of tropical medicine&hygiene,2010,104(9):623-624.
[5]SLAVIN G.‘Q’fever:the domestic animal as a source of infection for man[J].Veterinary record,1952,64:743-753.
[6]BENENSON A S,TIGERTT W D.Studies on Q fever in man[J].Transactions of the association of American physicians,1956,69:98-104.
[7]SIDI-BOUMEDINE K,ROUSSET E,HENNING K,et al.Development of harmonised schemes for the monitoring and reporting of Q-fever in animals in the European Union[J].EFSA scientific report,2010:1-48.
[8]SZYMA?SKA-CZERWI?SKA M,GALI?SKA E M,NIEMCZUK K,et al.Prevalence of Coxiella burnetii infection in humans occupationally exposed to animals in Poland[J].Vector-borne zoonotic diseases,2015,15(4):261-267.
[9]SZYMA?SKA-CZERWI?SKA M,GALI?SKA E M,NIEMCZUK K,et al.Prevalence of Coxiella burnetii infection in foresters and ticks in the south-eastern Poland and comparison of diagnostic methods[J].Annals of agricultural and environmental medicine,2013,20(4):699-704.
[10]武文君,贾广乐,廖娟红,等.Q热贝氏柯克斯体间接ELISA检测方法的建立[J].中国畜牧兽医,2014,41(4):95-101.
[11]KLEMMER J,NJERU J,EMAM A,et al.Q fever in Egypt:Epidemiological survey of Coxiella burnetii specific antibodies in cattle,buffaloes,sheep,goats and camels[J].Plos one.2018,13(2):e0192188.
[12]孙长俭,周连庆,薛文成,等.辽宁省农村地区Q热立克次体血清学及虫媒蜱携带病原体状况调查[J].华南国防医学杂志,2016,30(6):362-364.
[13]W E G D A M B L A N S M C,W I E L D E R S C C,MEEKELENKAMP J,et al.Evaluation of commonly used serological tests for detection of Coxiella burnetii antibodies in well-defined acute and follow-up sera[J].Clinical&vaccine immunology cvi,2012,19(7):1110-1115.
[14]OMSLAND A,COCKRELL D C,HOWE D,et al.Host cell-free growth of the Q fever bacterium Coxiella burnetii[J].Proceedings of the national academy of sciences of the United States of America.2009,106(11):4430-4434.
[15]MARES-GUIA,MARIA ANGéLIA M M,GUTERRES A,et al.Clinical and epidemiological use of nested PCRtargeting the repetitive element IS1111 associated with the transposase gene from Coxiella burnetii[J].Brazilian journal of microbiology,2018,49(1):138-143.
[16]亚红祥,张丽娟,张云智.Real-time PCR与常规PCR在Q热立克体检测中的比较[J].中国热带医学,2014,14(9):1054-1056.
[17]张琪,徐睿瑶,胡梦佳,等.贝氏柯克斯体可视化等温扩增法建立[J].中国公共卫生,2017,33(8):1219-1223.
[18]CHEN H W,CHING W M.Evaluation of the stability of lyophilized loop-mediated isothermal amplification reagents for the detection of Coxiella burnetii[J].Heliyon,2017,3(10):e00415.
[19]樊晓旭,赵永刚,李林,等.重组酶聚合酶扩增技术在疾病快速检测中的研究进展[J].中国动物检疫,2016,33(8):72-77.
[20]QI Y,YIN Q,SHAO Y,et al.Rapid and visual detection of Coxiella burnetii using recombinase polymerase amplification combined with lateral flow strips[J].Biomed research international,2018:6417354.
[21]KOO B,JIN C E,PARK S Y,et al.A rapid bio-optical sensor for diagnosing Q fever in clinical specimens[J].Biophotonics.2018,11:e201700167.
[22]MCLOUGHLIN K S.Microarrays for pathogen detection and analysis[J].Briefings in functional genomics,2011,10(6):342-353.
[23]焦俊.贝氏柯克斯体表面蛋白和T细胞表位的鉴定及免疫原性研究[D].北京:中国人民解放军军事医学科学院,2017.
[24]SCHMOOCK G,EHRICHT R,SPRAGUE L D.DNA microarray-based detection of Coxiella burnetii,the causative agent of Q fever[J].Acta veterinaria scandinavica,2014,56(1ya):27.
[25]王涛,宋立华.贝氏柯克斯体的分子致病机理研究进展[J].中国人兽共患病学报,2015,31(9):876-880.