miR-129抑制鼻咽癌细胞系CNE2增殖
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摘要
目的检测miR-129对鼻咽癌细胞系CNE2细胞生物学功能的影响。方法人工合成miR-129模拟物(mimics)和抑制物(inhibitor),转染至鼻咽癌CNE2细胞中,RT-qPCR检测细胞miR-129表达;CCK8法检测细胞增殖;Transwell小室侵袭实验检测细胞侵袭能力;流式细胞仪检测细胞凋亡能力。结果与对照组相比,转染miR-129 mimics组的miR-129表达水平明显升高(P<0.05);转染miR-129 inhibitor组的miR-129表达水平均明显降低(P<0.05);与对照组相比,转染miR-129 mimics组CNE2细胞增殖能力、侵袭能力显著下降(P<0.05),凋亡显著增多(P<0.05);与对照组相比,转染miR-129 inhibitor组CNE2细胞增殖能力、侵袭能力显著升高(P<0.05),凋亡显著下降(P<0.05)。结论 miR-129抑制鼻咽癌细胞系CNE2的增殖和迁移能力,促进细胞凋亡。
Objective To examine the effect of mir-129 on the biological function of nasopharyngeal carcinoma cells. Methods miR-129 mimics and inhibitor were synthesized and transfected into nasopharyngeal carcinoma cells. Mir-129 expression was detected by RT-PCR. Cell proliferation was detected by CCK8. Cell invasiveness was detected by Transwell test. Cell apoptosis was detected by flow cytometry. Results Compared with the control group, the expression of mir-129 in the mir-129 mimics group was significantly increased(P<0.05), and the expression of mir-129 was significantly reduced in the mir-129 inhibitor group(P<0.05). Compared with the control group, the proliferation and invasion of CNE2 cells in the mir-129 mimics group were significantly decreased(P<0.05), and apoptosis was significantly increased(P<0.05). Correspondingly the proliferation and invasion significantly increased(P<0.05) and apoptosis of CNE2 cells in the mir-129 inhibitor group was significantly decreased(P<0.05). Conclusions miR-129 inhibites the proliferation and migration in nasopharyngeal carcinoma cells and promotes apoptosis.
Objective To examine the effect of mir-129 on the biological function of nasopharyngeal carcinoma cells. Methods miR-129 mimics and inhibitor were synthesized and transfected into nasopharyngeal carcinoma cells. Mir-129 expression was detected by RT-PCR. Cell proliferation was detected by CCK8. Cell invasiveness was detected by Transwell test. Cell apoptosis was detected by flow cytometry. Results Compared with the control group, the expression of mir-129 in the mir-129 mimics group was significantly increased(P<0.05), and the expression of mir-129 was significantly reduced in the mir-129 inhibitor group(P<0.05). Compared with the control group, the proliferation and invasion of CNE2 cells in the mir-129 mimics group were significantly decreased(P<0.05), and apoptosis was significantly increased(P<0.05). Correspondingly the proliferation and invasion significantly increased(P<0.05) and apoptosis of CNE2 cells in the mir-129 inhibitor group was significantly decreased(P<0.05). Conclusions miR-129 inhibites the proliferation and migration in nasopharyngeal carcinoma cells and promotes apoptosis.
引文
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