桑叶不同提取物对肿瘤细胞的细胞毒性作用
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摘要
目的:研究桑叶不同提取物(水提物和醇提物)对肿瘤细胞的细胞毒性,为其抗肿瘤细胞研究提供实验依据。方法:运用四甲基偶氮唑蓝法(MTT法),以人肺癌A549细胞、人肝癌HepG2细胞、人胃癌SGC-7901细胞、人乳腺癌MCF-7细胞为研究对象,比较桑叶不同的提取物(水提物和醇提物)对四种肿瘤细胞的体外抗肿瘤活性。结果:桑叶水提物(AML)和醇提物(EML)对上述肿瘤细胞增殖均有不同程度的抑制作用,其中以醇提物的活性最强,且半数抑制浓度(IC_(50))均最小。结论:EML具有较高的体外抗肿瘤活性,AML次之,桑叶提取物有可能用于抗肿瘤治疗。
Objective:To study the tumor cytotoxity of different extracts of mulberry leaves,know its cytotoxic source and provide the experimental data for further study of anti-tumor cells activity. Method: By using MTT assay, A549, HepG2, SGC-7901, MCF-7 cells line were used to investigate their anti tumor activity in vitro.Results: The water extract and ethanol extract of Mulberry leaf showed different degrees of inhibition against four tumor cells, in which the most active fraction is ethanol extract with minimal IC_(50) value. Conclusions: The ethanol extract of Mulberry leaf showed good anti-tumor activity in vitro, followed by water extract. The extracts of mulberry leaf may be used for anti-tumor therapy.
Objective:To study the tumor cytotoxity of different extracts of mulberry leaves,know its cytotoxic source and provide the experimental data for further study of anti-tumor cells activity. Method: By using MTT assay, A549, HepG2, SGC-7901, MCF-7 cells line were used to investigate their anti tumor activity in vitro.Results: The water extract and ethanol extract of Mulberry leaf showed different degrees of inhibition against four tumor cells, in which the most active fraction is ethanol extract with minimal IC_(50) value. Conclusions: The ethanol extract of Mulberry leaf showed good anti-tumor activity in vitro, followed by water extract. The extracts of mulberry leaf may be used for anti-tumor therapy.
引文
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[2]原爱红,黄哲,马骏.桑叶黄酮的提取及降糖作用的研究[J].中草药,2004,35(11):1242-1243.
[3]滕美春,张如马,健雄,等.桑叶活性成分及临床应用研究进展[J].北方药学,2013,12(2):66-67.
[4] Yang S,Wang BL,Li Y. Advances in the pharmacological study of Morus alba L[J]. Acta Pharm Sin,2014,49(6):824-831.
[5]陈建国,步文磊,来伟旗,等.桑叶多糖降血糖作用及其机制研究[J].中草药,2011,42(3):515-520.
[6]宋喜云.新型复合桑业茶的研制进展[J].潍坊高等职业教育,2010,6(4):23-27.
[7] Bray F,Jemal A,Grey N,et al. Global cancer transitions according to the Human Development Index(2008-2030):a population-based study[J]. Oncology,2012,13(8):790-801.
[8] Kim S H,Liu C Y,Fan P W,et al. The aqueous extract of Brucea javanica suppresses cell growth and alleviates tumorigenesis of human lung cancer cells by targeting mutated epidermal growth factor receptor[J]. Drug Des Devel Ther,2016,10:3599-3609.
[9] Im M,Kim A,Ma J Y. Ethanol extract of baked Gardeniae Fructus exhibits in vitro and in vivo anti-metastatic and anti-angiogenic activities in malignant cancer cells:Role of suppression of the NF-κB and HIF-1αpathways[J]. Int J Oncol,2016,49(6):2377-2386.
[10] Wang X Y,Ding X,Yuan Y F,et al. Comprehensive two-dimensional APTES-decorated MCF7-cell membrane chromatographic system for characterizing potential anti-breast-cancer components from YuanhuBaizhi herbal medicine pair[J]. J Food Drug Anal,2018,26(2):823-833.
[11] Lian G,Li F,Yin Y,et al. Herbal extract of Artemisia vulgaris(mugwort)induces antitumor effects in HCT-15 human colon cancer cells via autophagy induction,cell migration suppression and loss of mitochondrial membrane potential[J]. J BUON,2018,23(1):73-78.
[12]李娟,唐东昕,杨柱,等.肺癌的中医药治疗与研究现状[J].贵阳中医学院学报,2017,39(3):98-101.
[13]陈启亮,唐东昕,龙奉玺.中医药对肝癌治疗的研究进展[J].贵阳中医学院学报,2016,38(2):99-101.
[14] Song SJ,Li LZ,Gao PY,et al. Terpenoids and hexenes from the leaves of Crataegus pinnatifida[J]. Food Chem,2011,129(3):933-939.
[15]王莉,杨永杰,归绥琪,等.掌叶半夏主要成分对子宫颈癌细胞生长的抑制作用[J].复旦大学学报:医学版,2009,36(6):675-680.
[16]戴开金.桑叶体外杭RSV及诱导Hela细胞凋亡物质基础研究[D].广州:南方医科大学,2009:82.
[17]王志雄,高剑文,缪伟伟.桑叶液对乳腺癌肿瘤细胞MDA-MB-231作用的研究[J].生物医学工程学进展,2015,36(1):25-29.
[18] Park S,Kim Y S,Lee H A,et al. Mulberry leaf extract inhibits invasive potential and downregulates hypoxia-inducible factor-1α(HIF-1α)in SK-N-BE2C neuroblastoma cells[J]. Biosci Biotechnol Biochem,2013,77(4):722-728.