P16和P53在子宫平滑肌肿瘤中的表达与意义
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摘要
目的目前已经有报道显示P16表达异常和子宫平滑肌肿瘤发生有密切关系,但在子宫平滑肌肿瘤中P16基因的作用似乎和大多数肿瘤有着明显的区别。为近一步探讨P16基因在子宫平滑肌肿瘤中的发生发展机制,本研究检测P16和P53在平滑肌肿瘤的表达情况。探讨P53和P16蛋白在子宫平滑肌肿瘤中的表达及临床意义。方法应用免疫组织化学方法检测20例良性子宫平滑肌瘤、11例子宫平滑肌肉及17例恶性潜能未定型子宫平滑肌瘤的P53和P16蛋白的表达情况。结果 P16蛋白在平滑肌肉瘤组的阳性表达率显著高于恶性潜能未定型平滑肌瘤组及平滑肌瘤组(P<0.05)。P53蛋白在平滑肌肉瘤组的阳性表达率显著高于平滑肌瘤组(P<0.05)。结论 P53和P16蛋白的异常表达与子宫平滑肌瘤向子宫平滑肌肉瘤的转化可能存在相关性,并为临床鉴别子宫平滑肌肿瘤良恶性提供参考依据。P16高表达促进了子宫平滑肌肿瘤恶性发生发展还是恶性潜能未定型平滑肌瘤和平滑肌肉瘤形成后P16的高表达仅仅是一种结果,有待进一步研究。
Objective It has been reported that P16 expression abnormality is closely related to uterine smooth muscle tumorigenesis,but the role of P16 gene in uterine smooth muscle tumor seems to be significantly different from most tumors. To further explore the mechanism of P16 gene development in uterine smooth muscle tumors,this study examined the expression of P16 and P53 in smooth muscle tumors. To investigate the expression and the clinical significance of P53 and P16 protein in human uterine smooth muscle tumors. Methods Immunohistochemical technique was used to detect the P53 and P16 protein expression in 20 benign leiomyoma,11 leiomyosarcoma and 17 smooth muscle tumors of uncertain malignant potential.Results The positive rates P16 protein expression in leiomyosarcoma was significantly higher than those in uncertain malignant potential and in usual leiomyoma( P<0.05). The positive rates P53 protein expression in leiomyosarcoma was significantly higher than in usual leiomyoma( P < 0. 05). Conclusions Abnormal expression of P53 and P16 proteins may be associated with the conversion of uterine leiomyoma to uterine leiomyosarcoma. And provide a reference for clinical identification of benign and malignant uterine smooth muscle tumors. High expression of P16 promotes the malignant development of uterine smooth muscle tumor or the high expression of P16 after the formation of malignant potential leiomyoma and leiomyosarcoma is only a result,which needs further study.
Objective It has been reported that P16 expression abnormality is closely related to uterine smooth muscle tumorigenesis,but the role of P16 gene in uterine smooth muscle tumor seems to be significantly different from most tumors. To further explore the mechanism of P16 gene development in uterine smooth muscle tumors,this study examined the expression of P16 and P53 in smooth muscle tumors. To investigate the expression and the clinical significance of P53 and P16 protein in human uterine smooth muscle tumors. Methods Immunohistochemical technique was used to detect the P53 and P16 protein expression in 20 benign leiomyoma,11 leiomyosarcoma and 17 smooth muscle tumors of uncertain malignant potential.Results The positive rates P16 protein expression in leiomyosarcoma was significantly higher than those in uncertain malignant potential and in usual leiomyoma( P<0.05). The positive rates P53 protein expression in leiomyosarcoma was significantly higher than in usual leiomyoma( P < 0. 05). Conclusions Abnormal expression of P53 and P16 proteins may be associated with the conversion of uterine leiomyoma to uterine leiomyosarcoma. And provide a reference for clinical identification of benign and malignant uterine smooth muscle tumors. High expression of P16 promotes the malignant development of uterine smooth muscle tumor or the high expression of P16 after the formation of malignant potential leiomyoma and leiomyosarcoma is only a result,which needs further study.
引文
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[10]徐小义,胡坚.食管癌中P53和Rb信号通路变化及临床意义[J].中华实验外科杂志,2016,2(33):505-508.
[11] Ismail A,Bandla S,BeveiUer M,et al.Early G cyclin-dependent kinasesas prognostic markers and potential therapeutic targets in esophageal adenocarcinoma[J]. Clin Cancer Res,2011,17(13):4513-4522.
[2] Kuman RJ,Carcangiu ML,Herrington CS,et al.WHO classification of tumours of female reproductive organs[M].4th ed. Lyon:IARC Press,2014:139-141.
[3] Kurman RJ,Ellendon LH,Ronnett BM.Blaustein pathology of the female genital[M].6th ed. Beijing Sci Technol Press,2014:459-462.
[4] Broaddus RR,Xie S,Hsu CJ,et al.The chemopreventive agents 4-HPR and DFMO inhibit growth and induce apoptosis in uterine leiomyomas[J].AM J Obstet Gynecol,2004,190(3):686-692.
[5] Bodner-Adler B,Bodner K,Czerwenka K,et al.Expression of P16protein in patients with uterine smooth muscle tumors:an immunohistochemical analysis[J]. Gynecol Oncol,2005,96(1):62-66.
[6] Brady CA,Attardi LD. P53 at a glance[J].J Cell Sci,2010,123(15):2527-2532.
[7] Bet termann K,Benesch M,Weis S,et al. Sumoylation in carcinogenesis[J].Cancer Lett,2012,316(12):113-125.
[8] Petrovi D,Babi D,Forko JI,et al.Expression of Ki-67,P53 and progesterone receptors in uterine smooth muscle tumors[J]. Coll Antropol,2010,34(1):93-97.
[9] O'Neill CJ, Mc Bride HA, Connolly LE, et al. Uterine leiomyosarcomas are characterized by high p16,p53 and MIB1expression in comparison with usual leiomyomas,leiomyoma variants and smooth muscle tumours of uncertain malignant potential[J].Histopathology,2007,50(7):851-858.
[10]徐小义,胡坚.食管癌中P53和Rb信号通路变化及临床意义[J].中华实验外科杂志,2016,2(33):505-508.
[11] Ismail A,Bandla S,BeveiUer M,et al.Early G cyclin-dependent kinasesas prognostic markers and potential therapeutic targets in esophageal adenocarcinoma[J]. Clin Cancer Res,2011,17(13):4513-4522.