头穴丛刺法对阿尔茨海默病大鼠学习记忆能力及海马CA1区炎症介质的影响
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摘要
目的:观察头穴丛刺法对阿尔茨海默病(AD)大鼠学习记忆能力及海马CA1区炎症介质的影响;方法:40只符合标准的SPF级Wistar大鼠随机分为空白组、假手术组、模型组和头穴丛刺组,每组10只。模型组和头穴丛刺组通过双侧海马区注射Aβ1-42溶液复制AD模型,假手术组注射等量无菌双氧水,空白组不做处置。头穴丛刺组于造模后2 d起给予针刺干预,空白组、假手术组和模型组仅背侧位捆绑固定,1次/d,连续治疗14 d。治疗结束后通过Morris水迷宫实验检测AD大鼠学习记忆能力,ELISA法检测海马CA1区中Ach、ChAT和AchE的含量和活性,免疫组化和RT-PCR法分别检测海马CA1区中IL-6、IL-10蛋白和mRNA的表达;结果:与空白组相比,模型组逃避潜伏期较空白组明显增加,跨越平台次数和有效停留时间较空白组明显降低,海马组织中Ach的含量、ChAT的活性、IL-10蛋白及mRNA的表达较空白组明显降低,AchE的活性和IL-6蛋白及mRNA的表达较空白组明显增加,差异均具有统计学意义(P<0.05)。与模型组比较,头穴丛刺能够显著降低大鼠逃避潜伏期时间,增加跨越平台次数和有效停留时间,增加海马组织中Ach的含量、ChAT的活性、IL-10蛋白及mRNA的表达,降低AchE的活性和IL-6蛋白及mRNA的表达,差异均具有统计学意义(P<0.05)。结论:头穴丛刺疗法能够通过调控AD大鼠海马CA1区组织中炎症因子的表达和促进Ach的含量,从而改善AD大鼠的学习记忆能力。
Objective: To observe the effects of cluster needling of scalp point therapy on learning and memory ability and inflammatory factors in hippocampal CA1 region of rats with Alzheimer's disease(AD). Methods: 40 SPF Wistar rats were randomly divided into control group, sham-operated group, model group and acupuncture group with 10 rats in each group. The AD model was replicated by injecting β-amyloid protein 1-42 solution into bilateral hippocampus in model group and acupuncture group. The sham-operated group was injected with the same amount of sterile hydrogen peroxide, while the control group was not disposed. The acupuncture group was treated with cluster needling of scalp point therapy after model establishment. The control group, sham operation group and model group were bundled and fixed in the dorsal position once a day for 14 consecutive days. After treatment, the learning and memory abilities of AD rats were tested by Morris water maze test, the contents and activities of Ach, ChAT and AchE in hippocampal CA1 region were detected by ELISA, and the expressions of IL-6, IL-10 protein and mRNA in hippocampal CA1 region were detected by immunohistochemistry and RT-PCR, respectively. Results: Compared with the control group, the escape latency of the model group was significantly increased(P<0.05), the number of crossing platforms and effective residence time were significantly decreased(P<0.05), the content of Ach, the activity of ChAT, the expression of IL-10 protein and mRNA in hippocampus were significantly decreased(P<0.05), and the activity of AchE and the expression of IL-6 protein and mRNA were significantly increased(P<0.05). Compared with the model group, cluster needling of scalp point therapy could significantly reduce the escape latency time(P<0.05), increase the number of crossing platforms and effective residence time(P<0.05), increase the content of Ach, the activity of ChAT, the expression of IL-10 protein and mRNA in hippocampus(P<0.05), and decrease the activity of AchE and the expression of IL-6 protein and mRNA(P<0.05). Conclusion: Scalp acupoint cluster needling therapy can improve the learning and memory ability of AD rats by regulating the expression of inflammatory mediators and promoting the content of Ach in hippocampal CA1 region of AD rats.
Objective: To observe the effects of cluster needling of scalp point therapy on learning and memory ability and inflammatory factors in hippocampal CA1 region of rats with Alzheimer's disease(AD). Methods: 40 SPF Wistar rats were randomly divided into control group, sham-operated group, model group and acupuncture group with 10 rats in each group. The AD model was replicated by injecting β-amyloid protein 1-42 solution into bilateral hippocampus in model group and acupuncture group. The sham-operated group was injected with the same amount of sterile hydrogen peroxide, while the control group was not disposed. The acupuncture group was treated with cluster needling of scalp point therapy after model establishment. The control group, sham operation group and model group were bundled and fixed in the dorsal position once a day for 14 consecutive days. After treatment, the learning and memory abilities of AD rats were tested by Morris water maze test, the contents and activities of Ach, ChAT and AchE in hippocampal CA1 region were detected by ELISA, and the expressions of IL-6, IL-10 protein and mRNA in hippocampal CA1 region were detected by immunohistochemistry and RT-PCR, respectively. Results: Compared with the control group, the escape latency of the model group was significantly increased(P<0.05), the number of crossing platforms and effective residence time were significantly decreased(P<0.05), the content of Ach, the activity of ChAT, the expression of IL-10 protein and mRNA in hippocampus were significantly decreased(P<0.05), and the activity of AchE and the expression of IL-6 protein and mRNA were significantly increased(P<0.05). Compared with the model group, cluster needling of scalp point therapy could significantly reduce the escape latency time(P<0.05), increase the number of crossing platforms and effective residence time(P<0.05), increase the content of Ach, the activity of ChAT, the expression of IL-10 protein and mRNA in hippocampus(P<0.05), and decrease the activity of AchE and the expression of IL-6 protein and mRNA(P<0.05). Conclusion: Scalp acupoint cluster needling therapy can improve the learning and memory ability of AD rats by regulating the expression of inflammatory mediators and promoting the content of Ach in hippocampal CA1 region of AD rats.
引文
1 刘亭,毕竟,王盼,等.MAPK/ERK信号通路参与褪黑素对阿尔茨海默病大鼠小脑的神经保护作用[J].中国药理学通报,2019,35(3):402-407.
2 王雨童,轩志东.阿尔茨海默的流行病学研究进展[J].中国实用神经疾病杂志,2015,18(20):118-119.
3 高文超,吕继辉,李沫,等.阿尔茨海默病患者行为精神症状与外周血单核细胞的相关性[J].中华老年多器官疾病杂志,2019(4):277-280.
4 李建忠,郝兴华,吴海平,等.丰富环境对阿尔茨海默病模型SAMP8小鼠海马CA1区炎症反应的影响[J].中国康复医学杂志,2018,33(3):274-279.
5 俞冰倩,张雪梅.阿尔茨海默病模型建立及应用的研究[J].脑与神经疾病杂志,2016,24(12):781-785.
6 马莉,王诗陶,顾兰馨.头穴丛刺法配合针刺八脉交会穴治疗血管性痴呆疗效观察[J].上海针灸杂志,2018,37(12):1373-1376.
7 徐飞,王玉珏,张可兴,等.头穴丛刺针法对AD患者脑内海马区代谢变化的影响[J].中国药物依赖性杂志,2018,27(2):110-113.
8 李虹霖,徐飞,夏昆鹏,等.头穴丛刺对阿尔茨海默病大鼠MAPK信号的影响[J].吉林中医药,2016,36(9):925-928.
9 俞冰倩,张雪梅.阿尔茨海默病模型建立及应用的研究[J].脑与神经疾病杂志,2016,24(12):781-785.
10 P G,W C.The rat brain in stereotaxic coordinates[M].6th ed.New York:Elsevier Academic Press,2007:78-80
11 李虹霖,徐飞,夏昆鹏,等.头穴丛刺对AD大鼠神经细胞凋亡的影响[J].长春中医药大学学报,2016,32(2):245-247.
12 王玲,朱鸣峰.阿尔茨海默病发病机制的研究进展[J].吉林医学,2013,34(3):531-532.
13 田杰,孙荣欣,王萍,等.阿尔茨海默病的发病机制研究进展[J].山东医药,2018,58(27):97-100.
14 黄幼异,黄秀峰,唐汉庆,等.积雪草水煎液预先灌胃的阿尔茨海默病模型小鼠学习记忆能力观察[J].山东医药,2018,58(43):43-46.
15 徐飞,王玉珏,张可兴,等.头穴丛刺针法对Aβ1-42致阿尔茨海默病大鼠学习记忆能力及胆碱能系统的影响[J].中国药物依赖性杂志,2018,27(3):202-205,224.
16 蒋持怡,艾琪,刘喆,等.电针改善慢性脑低灌注大鼠空间学习记忆能力和海马IL-6/JAK2/STAT3的信号调节[J].中国病理生理杂志,2018,34(7):1170-1176.
17 王渊,刘智斌,牛文民,等.嗅三针对阿尔茨海默病小鼠学习能力和海马IL1-β、IL-6表达的影响[J].四川中医,2018,36(9):33-36.
18 任莹,宗鑫,张亚楠,等.活血开窍方对转基因AD模型小鼠IL-1、IL-6及TNF-α的影响[J].山东中医药大学学报,2016,40(3):282-284.
19 陶一鸣,杜艳军,田青,等.电针对阿尔茨海默病大鼠齿状回星形胶质细胞和白细胞介素-10表达及其共定位的影响[J].中国老年学杂志,2019,39(4):874-878.
20 李冰,张军,吴蓓,等.IL-10对Aβ1-42诱导的阿尔茨海默病模型大鼠的保护作用[J].江苏医药,2012,38(18):2116-2118,2108.
21 刘晓雯.阿尔茨海默病患者血清Hcy、IL-6、VEGF、SOD水平与认知功能、生活质量的相关性研究[J].社区医学杂志,2018,16(8):34-36.
22 李冰,毛丰,方小霞,等.白细胞介素-10对冈田酸诱导的阿尔茨海默病模型大鼠的保护作用[J].南京医科大学学报(自然科学版),2013,33(7):932-936.
2 王雨童,轩志东.阿尔茨海默的流行病学研究进展[J].中国实用神经疾病杂志,2015,18(20):118-119.
3 高文超,吕继辉,李沫,等.阿尔茨海默病患者行为精神症状与外周血单核细胞的相关性[J].中华老年多器官疾病杂志,2019(4):277-280.
4 李建忠,郝兴华,吴海平,等.丰富环境对阿尔茨海默病模型SAMP8小鼠海马CA1区炎症反应的影响[J].中国康复医学杂志,2018,33(3):274-279.
5 俞冰倩,张雪梅.阿尔茨海默病模型建立及应用的研究[J].脑与神经疾病杂志,2016,24(12):781-785.
6 马莉,王诗陶,顾兰馨.头穴丛刺法配合针刺八脉交会穴治疗血管性痴呆疗效观察[J].上海针灸杂志,2018,37(12):1373-1376.
7 徐飞,王玉珏,张可兴,等.头穴丛刺针法对AD患者脑内海马区代谢变化的影响[J].中国药物依赖性杂志,2018,27(2):110-113.
8 李虹霖,徐飞,夏昆鹏,等.头穴丛刺对阿尔茨海默病大鼠MAPK信号的影响[J].吉林中医药,2016,36(9):925-928.
9 俞冰倩,张雪梅.阿尔茨海默病模型建立及应用的研究[J].脑与神经疾病杂志,2016,24(12):781-785.
10 P G,W C.The rat brain in stereotaxic coordinates[M].6th ed.New York:Elsevier Academic Press,2007:78-80
11 李虹霖,徐飞,夏昆鹏,等.头穴丛刺对AD大鼠神经细胞凋亡的影响[J].长春中医药大学学报,2016,32(2):245-247.
12 王玲,朱鸣峰.阿尔茨海默病发病机制的研究进展[J].吉林医学,2013,34(3):531-532.
13 田杰,孙荣欣,王萍,等.阿尔茨海默病的发病机制研究进展[J].山东医药,2018,58(27):97-100.
14 黄幼异,黄秀峰,唐汉庆,等.积雪草水煎液预先灌胃的阿尔茨海默病模型小鼠学习记忆能力观察[J].山东医药,2018,58(43):43-46.
15 徐飞,王玉珏,张可兴,等.头穴丛刺针法对Aβ1-42致阿尔茨海默病大鼠学习记忆能力及胆碱能系统的影响[J].中国药物依赖性杂志,2018,27(3):202-205,224.
16 蒋持怡,艾琪,刘喆,等.电针改善慢性脑低灌注大鼠空间学习记忆能力和海马IL-6/JAK2/STAT3的信号调节[J].中国病理生理杂志,2018,34(7):1170-1176.
17 王渊,刘智斌,牛文民,等.嗅三针对阿尔茨海默病小鼠学习能力和海马IL1-β、IL-6表达的影响[J].四川中医,2018,36(9):33-36.
18 任莹,宗鑫,张亚楠,等.活血开窍方对转基因AD模型小鼠IL-1、IL-6及TNF-α的影响[J].山东中医药大学学报,2016,40(3):282-284.
19 陶一鸣,杜艳军,田青,等.电针对阿尔茨海默病大鼠齿状回星形胶质细胞和白细胞介素-10表达及其共定位的影响[J].中国老年学杂志,2019,39(4):874-878.
20 李冰,张军,吴蓓,等.IL-10对Aβ1-42诱导的阿尔茨海默病模型大鼠的保护作用[J].江苏医药,2012,38(18):2116-2118,2108.
21 刘晓雯.阿尔茨海默病患者血清Hcy、IL-6、VEGF、SOD水平与认知功能、生活质量的相关性研究[J].社区医学杂志,2018,16(8):34-36.
22 李冰,毛丰,方小霞,等.白细胞介素-10对冈田酸诱导的阿尔茨海默病模型大鼠的保护作用[J].南京医科大学学报(自然科学版),2013,33(7):932-936.