电感耦合等离子体发射光谱法测定蜂蜜中7种矿物元素的含量
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摘要
为了快速直接测定蜂蜜中多种矿物元素的含量,采用电感耦合等离子体发射光谱(ICP-AES)法建立了同时测定蜂蜜中钙、钾、镁、钠、锌、铁、铜7种矿物元素含量的方法,考察了方法的标准曲线线性关系、检出限、精密度和准确度,讨论了前处理方法、不同产地以及蜜源植物种类对各元素含量的影响。结果表明:线性相关系数达到0.999 9以上,方法检出限为0.003～0.030 mg/L,相对标准偏差低于2%,回收率为88.66%～105.31%;不同产地的洋槐蜜中,河北洋槐蜜的钙、钾含量明显高于其他地区,广西洋槐蜜的钠、铁含量最高;不同蜜源植物的蜂蜜中,河北枣花蜜的铁含量最高,野生蜜中各种矿物元素都较为丰富。电感耦合等离子体发射光谱法可以快速、同时测定蜂蜜中多种矿物元素含量,方法简便易行,结果准确可靠,可为蜂蜜品质鉴定以及人们对蜂蜜的合理选择提供参考。
In order to determine the contents of various mineral elements in honey rapidly and directly, an experimental method for simultaneous determination of Ca, K, Mg, Na, Zn, Fe, Cu in honey by inductively coupled plasma emission spectrometry(ICP-AES) is established. The method′s standard curve linear relation, detection limit, precision and accuracy degrees are investigated. The influence of pretreatment method, different producing areas, and honey plant floristics on different elements contents is discussed. The results show that the linear correlation coefficient of each element standard curves is over 0.999 9. The detection limit of the method is 0.003～0.030 mg/L. The RSD is less than 2%. The recovery rate of each element is 88.66%～105.31%. Ca and K contents in acacia honey from Hebei are significantly higher than those in other areas, while the Na and Fe contents in acacia honey from Guangxi are the highest. Hebei jujube nectar has the highest Fe content in different honey plants and wild honey from Hebei is abundant in various mineral elements. ICP-AES is a simple and reliable method for the simultaneous determination of mineral elements in honey.ICP-AES can determine the content of various mineral elements in honey rapidly and simultaneously. The method is simple and easy to operate, and the results are accurate and reliable, which can provide reference for honey quality identification and selection of honey species.
In order to determine the contents of various mineral elements in honey rapidly and directly, an experimental method for simultaneous determination of Ca, K, Mg, Na, Zn, Fe, Cu in honey by inductively coupled plasma emission spectrometry(ICP-AES) is established. The method′s standard curve linear relation, detection limit, precision and accuracy degrees are investigated. The influence of pretreatment method, different producing areas, and honey plant floristics on different elements contents is discussed. The results show that the linear correlation coefficient of each element standard curves is over 0.999 9. The detection limit of the method is 0.003～0.030 mg/L. The RSD is less than 2%. The recovery rate of each element is 88.66%～105.31%. Ca and K contents in acacia honey from Hebei are significantly higher than those in other areas, while the Na and Fe contents in acacia honey from Guangxi are the highest. Hebei jujube nectar has the highest Fe content in different honey plants and wild honey from Hebei is abundant in various mineral elements. ICP-AES is a simple and reliable method for the simultaneous determination of mineral elements in honey.ICP-AES can determine the content of various mineral elements in honey rapidly and simultaneously. The method is simple and easy to operate, and the results are accurate and reliable, which can provide reference for honey quality identification and selection of honey species.
引文
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[14] 黎淑端,潘心红,谢进,等.用原子荧光分光法测定陶瓷餐具中锑的含量[J].中国卫生检验杂志,2019,29(3):278-279.LI Shuduan,PAN Xinhong,XIE Jin,et al.Determination of antimony in ceramic dishware by atomic fluorescence spectrometry[J].Chinese Journal of Health Laboratory Technology,2019,29(3):278-279.
[15] 陶琛,李春生,初威澄,等.非色散原子荧光光谱法同时检测硒和铅的光源干扰校正方法研究[J].分析化学,2019,47(1):163-168.TAO Chen,LI Chunsheng,CHU Weicheng,et al.Correction method of light source interference for simultaneous determination of selenium and lead by non-dispersive hydride generation-atomic fluorescence spectrometry[J].Chinese Journal of Analytical Chemistry,2019,47(1):163-168.
[16] 郭岚,王蕊,柳英霞,等.电感耦合等离子体-原子发射光谱法同时测定不同种类蜂蜜中的20种微量元素[J].分析化学学报,2011,27(4):530-532.GUO Lan,WANG Rui,LIU Yingxia,et al.Simultaneous determination of 20 trace elements in defferernt kinds of honey by inductively coupled plasma-atomic emission spectrometry[J].Journal of Analytical Science,2011,27(4):530-532.
[17] 张帆,王浩杰,蔡薇,等.沉淀分离后电感耦合等离子体发射光谱法分析99.99%银中的杂质元素[J].生物化工,2018,4(6):106-107.ZHANG Fan,WANG Haojie,CAI Wei,et al.Determination of impurity elements in 99.99% silver by ICP-AES after precipitation separation[J].Biological Chemical Engineering,2018,4(6):106-107.
[18] 胡伟,马俊辉,张晓飞,等.电感耦合等离子体发射光谱法测定食品接触用无机材料及制品中13种重金属迁移量[J].食品安全质量检测学报,2018,9(21):5743-5748.HU Wei,MA Junhui,ZHANG Xiaofei,et al.Determination of 13 heavy metals migration in food contact inorganic materials and products by inductively coupled plasma atomic emission spectrometry[J].Journal of Food Safety and Quality,2018,9(21):5743-5748.
[19] SILVA M A,CANHA N,GALINHA C,et al.Trace elements in wild and orchard honeys[J].Applied Radiation and Isotopes,2011,69(11):1592-1595.
[2] 刘鹏.蜂蜜内部营养成分及有毒有害物质的调查及研究进展[J].现代食品,2017(4):55-57.LIU Peng.The progress of the investigation and research of honey internal nutrients and toxic and harmful substances[J].Modern Food,2017(4):55-57.
[3] 汪思凡,曹振辉,潘洪彬,等.蜂蜜化学成分及其主要生物学功能研究进展[J].食品研究与开发,2018,39(1):176-181.WANG Sifan,CAO Zhenhui,PAN Hongbin,et al.Research progress on chemical composition and major biological function of honey [J].Food Research and Development,2018,39(1):176-181.
[4] 王福俤.中国生物微量元素研究的现状与展望[J].生命科学,2012,24(8):713-730.WANG Fudi.Trace element research in China:Present and future[J].Chinese Bulletin of Life Sciences,2012,24(8):713-730.
[5] 郑亚杰,刘秀斌,林莉,等.蜂蜜中植物源性毒性成分的研究进展[J].食品科学,2018,29(5):1-13.ZHENG Yajie,LIU Xiubin,LIN Li,et al.Plant-derived toxins in honey:A review[J].Food Science,2018,29(5):1-13.
[6] PISANI A,PROTANO G,RICCOBONO F.Minor and trace elements in different honey types produced in Siena County (Italy)[J].Food Chemistry,2008,107(4):1553-1560.
[7] 周洁,王佳芷,黄林生,等.原子吸收光谱法测定武当山地区樱桃中重金属含量[J].食品工业,2018,39(11):318-321.ZHOU Jie,WANG Jiazhi,HUANG Linsheng,et al.Determination of heavy metals content in Wudang cherry by atomic absorption spectrometry[J].The Food Industry,2018,39(11):318-321.
[8] 周晨露.原子吸收光谱法在水质重金属检测中的应用探讨[J].当代化工研究,2018(10):118-119.ZHOU Chenlu.Exploration on the application of atomic absorption spectrometry in the heavy metals determination in water quality[J].Chemical Intermediate,2018(10):118-119.
[9] 胡庆兰,周渊,邓樱花,等.微波消解-火焰原子吸收光谱法测定无茎芥中微量金属元素[J].应用化工,2017,46(3):586-588.HU Qinglan,ZHOU Yuan,DENG Yinghua,et al.FAAS determination of trace metal elements in no stem mustard with microwave assisted digestion[J].Applied Chemical Industry,2017,46(3):586-588.
[10] CALLE I L,PEREIRA F P,LAVILLA I,et al.Liquid-phase microextraction combined with graphite furnace atomic absorption spectrometry:A review[J].Analytica Chimica Acta,2016,936:12-39.
[11] 陈辉,范春林,常巧英,等.ICP-MS测定蜂蜜中多元素结合化学计量学在不同产地荆条蜜溯源中的应用[J].光谱学与光谱分析,2015,35(1):212-216.CHEN Hui,FAN Chunlin,CHANG Qiaoying,et al.Application of ICP-MS method in the determination of mineral elements in vitex honey for the classification of their geographical origins with chemometric approach[J].Spectroscopy and Spectral Analysis,2015,35(1):212-216.
[12] 李宗芮,徐丽,徐颖,等.蜂蜜中元素含量检测的两种前处理方法比较[J].食品安全质量检测学报,2017,8(11):4195-4201.LI Zongrui,XU Li,XU Ying,et al.Comparison of two pretreatment methods of element content detection in honey[J].Journal of Food Safety and Quality,2017,8(11):4195-4201.
[13] 孙传强,龚子珊,郭广宏,等.直接稀释/电感耦合等离子体质谱法测定人血清中微量元素[J].分析测试学报,2018,37(3):346-349.SUN Chuanqiang,GONG Zishan,GUO Guanghong,et al.Determination of trace elements in human serum by inductively coupled plasma mass spectrometry with direct dilution method[J].Journal of Instrumental Analysis,2018,37(3):346-349.
[14] 黎淑端,潘心红,谢进,等.用原子荧光分光法测定陶瓷餐具中锑的含量[J].中国卫生检验杂志,2019,29(3):278-279.LI Shuduan,PAN Xinhong,XIE Jin,et al.Determination of antimony in ceramic dishware by atomic fluorescence spectrometry[J].Chinese Journal of Health Laboratory Technology,2019,29(3):278-279.
[15] 陶琛,李春生,初威澄,等.非色散原子荧光光谱法同时检测硒和铅的光源干扰校正方法研究[J].分析化学,2019,47(1):163-168.TAO Chen,LI Chunsheng,CHU Weicheng,et al.Correction method of light source interference for simultaneous determination of selenium and lead by non-dispersive hydride generation-atomic fluorescence spectrometry[J].Chinese Journal of Analytical Chemistry,2019,47(1):163-168.
[16] 郭岚,王蕊,柳英霞,等.电感耦合等离子体-原子发射光谱法同时测定不同种类蜂蜜中的20种微量元素[J].分析化学学报,2011,27(4):530-532.GUO Lan,WANG Rui,LIU Yingxia,et al.Simultaneous determination of 20 trace elements in defferernt kinds of honey by inductively coupled plasma-atomic emission spectrometry[J].Journal of Analytical Science,2011,27(4):530-532.
[17] 张帆,王浩杰,蔡薇,等.沉淀分离后电感耦合等离子体发射光谱法分析99.99%银中的杂质元素[J].生物化工,2018,4(6):106-107.ZHANG Fan,WANG Haojie,CAI Wei,et al.Determination of impurity elements in 99.99% silver by ICP-AES after precipitation separation[J].Biological Chemical Engineering,2018,4(6):106-107.
[18] 胡伟,马俊辉,张晓飞,等.电感耦合等离子体发射光谱法测定食品接触用无机材料及制品中13种重金属迁移量[J].食品安全质量检测学报,2018,9(21):5743-5748.HU Wei,MA Junhui,ZHANG Xiaofei,et al.Determination of 13 heavy metals migration in food contact inorganic materials and products by inductively coupled plasma atomic emission spectrometry[J].Journal of Food Safety and Quality,2018,9(21):5743-5748.
[19] SILVA M A,CANHA N,GALINHA C,et al.Trace elements in wild and orchard honeys[J].Applied Radiation and Isotopes,2011,69(11):1592-1595.