小儿久咳合剂对感染后咳嗽(肺热阴虚证)大鼠咳嗽敏感性及血清IL-5、TNF-α、IgE含量的影响
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摘要
目的:观察小儿久咳合剂对感染后咳嗽(肺热阴虚证)大鼠咳嗽敏感性及血清中IL-5、TNF-α、IgE含量的影响,探讨该药降低咳嗽敏感性,减轻气道炎性反应的作用机制。方法:从50只SD大鼠中随机选取10只作为空白组,其余40只采用烟熏+内毒素滴鼻+甲状腺素灌胃+辣椒素雾化诱咳法建立感染后咳嗽(肺热阴虚证)大鼠模型,造模成功后随机分为模型组、孟鲁司特钠组、小儿久咳合剂小剂量组及小儿久咳合剂大剂量组,每组10只。各组分别予以相应药物进行干预,空白组及模型组则均给予同体积的0.9%氯化钠溶液灌胃。多功能诱咳引喘仪检测各组大鼠咳嗽敏感性,ELISA法检测治疗前后各组大鼠血清中IL-5、TNF-α、IgE水平。结果:与模型组比较,小儿久咳合剂大、小剂量组大鼠咳嗽频次明显减少,血清中IL-5、TNF-α、IgE水平也明显降低(P<0.05,P<0.01)。结论:小儿久咳合剂能降低模型大鼠的咳嗽敏感性,减少模型大鼠血清中IL-5、TNF-α和IgE等炎性介质的释放,从而减轻气道炎性反应。
Objective: To observe the effects of Pediatric Chronic Cough Mixture(PCCM) on cough sensitivity and the content of IL-5, TNF-α and IgE in post infectious cough(PIC) rats with syndrome of yin deficiency due to lung heat and explore the mechanism. Methods: The fifty rats were enrolled and randomly divided into control group(n=10) and model group(n=40). The PIC(syndrome of yin deficiency due to lung heat) model rats was established by cigarette-smoking exposure, intranasal administration with small dose of LPS, thyroid hormone and capsaicin atomization method. Then, the model rats were enrolled and randomly divided into model group(n=10), montelukast sodium group(n=10), large PCCM group with low doses(n=10) and high doses(n=10). 0.9%Saline, montelukast sodium suspension and PPCM was given to the rats in corresponding group as the therapeutic drug. When the treatment was finished, the cough sensitivity and the contents of IL-5, TNF-α and IgE in serum of rats were determined. Results: Compared with the model group, the cough frequency and the content of IL-5, TNF-α and IgE in serum of rats in large PCCM group with low doses and high doses were declined(P<0.05, P<0.01). Conclusion: PPCM can reduce cough sensitivity of the model rats. PPCM might treat PIC(syndrome of yin deficiency due to lung heat) by reducing the release of IL-5, TNF-α, IgE and other inflammatory mediators in serum.
Objective: To observe the effects of Pediatric Chronic Cough Mixture(PCCM) on cough sensitivity and the content of IL-5, TNF-α and IgE in post infectious cough(PIC) rats with syndrome of yin deficiency due to lung heat and explore the mechanism. Methods: The fifty rats were enrolled and randomly divided into control group(n=10) and model group(n=40). The PIC(syndrome of yin deficiency due to lung heat) model rats was established by cigarette-smoking exposure, intranasal administration with small dose of LPS, thyroid hormone and capsaicin atomization method. Then, the model rats were enrolled and randomly divided into model group(n=10), montelukast sodium group(n=10), large PCCM group with low doses(n=10) and high doses(n=10). 0.9%Saline, montelukast sodium suspension and PPCM was given to the rats in corresponding group as the therapeutic drug. When the treatment was finished, the cough sensitivity and the contents of IL-5, TNF-α and IgE in serum of rats were determined. Results: Compared with the model group, the cough frequency and the content of IL-5, TNF-α and IgE in serum of rats in large PCCM group with low doses and high doses were declined(P<0.05, P<0.01). Conclusion: PPCM can reduce cough sensitivity of the model rats. PPCM might treat PIC(syndrome of yin deficiency due to lung heat) by reducing the release of IL-5, TNF-α, IgE and other inflammatory mediators in serum.
引文
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[14]张海龙,裴月湖,华会明.诃子化学成分及药理活性的研究进展.沈阳药科大学学报,2001,18(6):452
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[16]Heikkinen T,Jarvinen A.The common cold.Labcet,2003,361(9351):51-59
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[19]To Y,Dohi Matanuska R,Tanaka R,et al.Early interleukin-4dependent response can induce airway hyperreactivity before development of airway inflammation in a mouse model of asthma.Lab Invest,2001,81:1385-1396
[20]Betz R,Kohlhaufl M,Kassner G,et al.Increased sputum IL-8 and IL-5 in asymptomatic nonspecific airway hyperresponsiveness.Lung,2001,179:119-133
[21]张慎峰.气道高反应性的研究进展.黑龙江医学,2003,27(10):749-750
[2]黄秋芳.小儿感染后咳嗽的发病机理及治疗进展.中国药业,2009,18(15):84-87
[3]荀春铮.小儿久咳方治疗儿童感染后慢性咳嗽110例疗效观察.中医药导报,2014,20(3):101-102
[4]周婷,王孟清,罗银河.肺热阴虚型感染后慢性咳嗽大鼠模型的建立及评价.中医药导报,2016,22(5):16-20
[5]周德文,李长敏,尹玲豫.桑白皮的药理活性.国外医药.植物药分册,1997,3(12):115-116
[6]公衍玲,金宏,王宏波.侧柏叶挥发油提取工艺及其抑菌活性研究.化学与生物工程,2009,26(2):36
[7]宁娜,韩建军.地骨皮的化学成分与药理作用.现代药物与临床,2010,25(3):25
[8]辛晓明,张倩,王浩,等.南沙参的化学成分及药效学研究进展.中国实用医药,2008,28(3):188-189
[9]黄光辉,孙莲娜.麦冬多糖的研究进展.现代药物与临床,2012,27(5):69
[10]薛俊超.白花前胡化学成分及相关药理作用的研究进展.海峡药学,2012,24(2):64
[11]张建伟,窦昌贵,张勉,等.紫菀、款冬及其配伍的毒性及药效学研究.中国临床药理学与治疗学,2007,12(4):405-406
[12]刘传安,邹盛勤,陈武.枇杷叶化学成分、药理作用及其应用研究进展.安徽农业科学,2005,33(11):138
[13]黄荣增,吴娟,陶群,等.地龙提取物柱层析各部位的平喘活性研究.中国药师,2010,13(11):324
[14]张海龙,裴月湖,华会明.诃子化学成分及药理活性的研究进展.沈阳药科大学学报,2001,18(6):452
[15]范存伟,李珍.补中益气汤主要药物的新药理作用.医药产业资讯,2005,15(2):127-128
[16]Heikkinen T,Jarvinen A.The common cold.Labcet,2003,361(9351):51-59
[17]Johnston S J.Overview of virus-induced airway disease.Proc Am Thorac Society,2005,2(2):150-156
[18]Fujimori K,Suzuki E,Arakawa M.Clinical features of post infectious chronic cough.Arerugi,1997,46(5):420-425
[19]To Y,Dohi Matanuska R,Tanaka R,et al.Early interleukin-4dependent response can induce airway hyperreactivity before development of airway inflammation in a mouse model of asthma.Lab Invest,2001,81:1385-1396
[20]Betz R,Kohlhaufl M,Kassner G,et al.Increased sputum IL-8 and IL-5 in asymptomatic nonspecific airway hyperresponsiveness.Lung,2001,179:119-133
[21]张慎峰.气道高反应性的研究进展.黑龙江医学,2003,27(10):749-750