乌梢蛇鉴别技术研究
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摘要
乌梢蛇,出自《本草纲目》,又名乌蛇,始载于《开宝本草》,主要在中国分布,国外未见报道。乌梢蛇作为的临床功效确切的中药材,其质量鉴别技术至关重要。全文就20年来鉴别技术的研究发展,从传统技术和现代鉴别技术2个方面阐述,主要包括性状、显微、色谱法、PCR技术以及电泳等鉴别方法。对近年来乌梢蛇基于PCR技术,开发一系列高效、准确的分子水平的鉴别方法及基于化学成分分析,高效毛细管电泳技术的应用进行了总结概述,为以后蛇类鉴别技术的发展以及研究奠定了基础,也为动物药的质量保证的提供参考。
The Zaocys, from the Ben Cao Gang Mu, also known as the black snake. It starts with Kai Bao Ben Cao. It is mainly distributed in China and has not been reported abroad. The clinical efficacy of Zaocys as the exact Chinese herbal medicine, its quality identification technology is very important. The full text of the research and development of the identification technology for the past 20 years, explain from two aspects: traditional technology and modern identification technology, it mainly includes identification methods such as character, micrograph, chromatography, PCR technology and electrophoresis. For recent years, the black snake adopts PCR technology to develop a series of efficient and accurate molecular level identification methods and chemical composition analysis and the application of high-performance capillary electrophoresis technology is summarized. It laid the foundation for the development and research of snake identification technology and also provide reference for quality assurance of animal medicine.
The Zaocys, from the Ben Cao Gang Mu, also known as the black snake. It starts with Kai Bao Ben Cao. It is mainly distributed in China and has not been reported abroad. The clinical efficacy of Zaocys as the exact Chinese herbal medicine, its quality identification technology is very important. The full text of the research and development of the identification technology for the past 20 years, explain from two aspects: traditional technology and modern identification technology, it mainly includes identification methods such as character, micrograph, chromatography, PCR technology and electrophoresis. For recent years, the black snake adopts PCR technology to develop a series of efficient and accurate molecular level identification methods and chemical composition analysis and the application of high-performance capillary electrophoresis technology is summarized. It laid the foundation for the development and research of snake identification technology and also provide reference for quality assurance of animal medicine.
引文
[1]林喆,胡丽娜,李娜.动物药整理研究:乌梢蛇[J].吉林中医药,2009,29(11):982-984.
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[3]杨小平,屈菊兰,林劲松.中药乌梢蛇的光镜及扫描电镜鉴别研究[J].中草药,1999(8):613-615.
[4]熊学敏,康明,曹珏.纯蛇粉胶囊中乌梢蛇的薄层层析鉴别[J].蛇志,1998(4):6-7.
[5]张乐,陶明宝,陈鸿平,等.常用蛇类药材鉴别研究进展[J].中国实验方剂学杂志,2017,23(4):222-227.
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[7]陈康,蒋超,袁媛,等.快速PCR方法在蛇类药材真伪鉴别中的应用[J].中国中药杂志,2014,39(19):3673-3677.
[8]蒋超,黄璐琦,袁媛,等.使用碱裂解法快速提取药材DNA方法的研究[J].药物分析杂志,2013,33(7):1081.
[9]Wang Y-q,Zhouk Y.A preliminary study on the identifi ca-tion of crude snake drugs bymolecular genetic markers[J].Acta Pharm Sin,1997,32(5):384-387.
[10]薛敬伟,何玲,赵氚,等.荧光检测技术在蛋白酪氨酸激酶抑制剂高通量筛选中的应用[J].药学进展,2012,36(1):28-33.
[11]胡永领,刘成永,成松.应用RNA恒温扩增实时荧光检测技术观察抗结核药物的疗效[J].海峡药学,2015,27(1):75-77.
[12]张海晴,黄海滨,王春颖,等.RNA恒温扩增实时荧光检测技术在检测脑脊液中结核分枝杆菌的应用[J].广东医学,2017,38(11):1715-1716,1719
[13]Wernike K,Beer M,Hoff mann B.Rapid detection of footand-mouth disease virus,influenza A virus and classical swine fevervirus by high-speed real-time RT-PCR[J].Journal of Virological Methods,2013,193(1):50.
[14]Lee H Y,Park M J,Kim N Y,et al.Rapid direct PCR for ABO blood typing[J].Journal of Forensic Sciences,2011,56(1):179-182.
[15]唐晓晶,冯成强,黄璐琦,等.高特异性PCR方法鉴别乌梢蛇及其混淆品[J].中国药学杂志,2007,42(5):333-336.
[16]孙清萍,陈素珍,盛英美.快速检测中药材乌梢蛇LAMP方法的建立[J].氨基酸和生物资源,2016,38(3):36-39.
[17]郝顺祖.一种快速提取DNA的方法[J].江苏大学学报:医学版,1995,5(1):55.
[18]杨兰泽,高静.一种简单、快速提取DNA的方法[J].生命的化学,1999,19(4):183.
[19]李梓僮,孙景昱,周亭亭,等.乌梢蛇DNA检测试剂盒的研制与应用[J].中国药学杂志,2017,52(9):777-781.
[20]国家药典编辑委员会.中国药典[M].北京:中国医药科技出版社,2015.
[21]郑艳青,王艳敏.乌梢蛇的化学成分及分析方法研究进展[J].中国药业,2006,15(21):59-60.
[22]李峰,张阳,张振秋,等.乌梢蛇药材的高效毛细管电泳指纹图谱研究[J].辽宁中医杂志,2015,42(10):1953-1954.
[23]肖寄平,黄青,陈人萍,等.乌梢蛇及其常见伪品的鉴别研究[J].吉林中医药,2009,29(10):884-885.
[2]宋秀菊.动物乌梢蛇的鉴别[J].时珍国医国药,2012,23(7):1824.
[3]杨小平,屈菊兰,林劲松.中药乌梢蛇的光镜及扫描电镜鉴别研究[J].中草药,1999(8):613-615.
[4]熊学敏,康明,曹珏.纯蛇粉胶囊中乌梢蛇的薄层层析鉴别[J].蛇志,1998(4):6-7.
[5]张乐,陶明宝,陈鸿平,等.常用蛇类药材鉴别研究进展[J].中国实验方剂学杂志,2017,23(4):222-227.
[6]国家药典委员会.中国药典:1部[M].北京:中国医药科技出版社,2010:72-73.
[7]陈康,蒋超,袁媛,等.快速PCR方法在蛇类药材真伪鉴别中的应用[J].中国中药杂志,2014,39(19):3673-3677.
[8]蒋超,黄璐琦,袁媛,等.使用碱裂解法快速提取药材DNA方法的研究[J].药物分析杂志,2013,33(7):1081.
[9]Wang Y-q,Zhouk Y.A preliminary study on the identifi ca-tion of crude snake drugs bymolecular genetic markers[J].Acta Pharm Sin,1997,32(5):384-387.
[10]薛敬伟,何玲,赵氚,等.荧光检测技术在蛋白酪氨酸激酶抑制剂高通量筛选中的应用[J].药学进展,2012,36(1):28-33.
[11]胡永领,刘成永,成松.应用RNA恒温扩增实时荧光检测技术观察抗结核药物的疗效[J].海峡药学,2015,27(1):75-77.
[12]张海晴,黄海滨,王春颖,等.RNA恒温扩增实时荧光检测技术在检测脑脊液中结核分枝杆菌的应用[J].广东医学,2017,38(11):1715-1716,1719
[13]Wernike K,Beer M,Hoff mann B.Rapid detection of footand-mouth disease virus,influenza A virus and classical swine fevervirus by high-speed real-time RT-PCR[J].Journal of Virological Methods,2013,193(1):50.
[14]Lee H Y,Park M J,Kim N Y,et al.Rapid direct PCR for ABO blood typing[J].Journal of Forensic Sciences,2011,56(1):179-182.
[15]唐晓晶,冯成强,黄璐琦,等.高特异性PCR方法鉴别乌梢蛇及其混淆品[J].中国药学杂志,2007,42(5):333-336.
[16]孙清萍,陈素珍,盛英美.快速检测中药材乌梢蛇LAMP方法的建立[J].氨基酸和生物资源,2016,38(3):36-39.
[17]郝顺祖.一种快速提取DNA的方法[J].江苏大学学报:医学版,1995,5(1):55.
[18]杨兰泽,高静.一种简单、快速提取DNA的方法[J].生命的化学,1999,19(4):183.
[19]李梓僮,孙景昱,周亭亭,等.乌梢蛇DNA检测试剂盒的研制与应用[J].中国药学杂志,2017,52(9):777-781.
[20]国家药典编辑委员会.中国药典[M].北京:中国医药科技出版社,2015.
[21]郑艳青,王艳敏.乌梢蛇的化学成分及分析方法研究进展[J].中国药业,2006,15(21):59-60.
[22]李峰,张阳,张振秋,等.乌梢蛇药材的高效毛细管电泳指纹图谱研究[J].辽宁中医杂志,2015,42(10):1953-1954.
[23]肖寄平,黄青,陈人萍,等.乌梢蛇及其常见伪品的鉴别研究[J].吉林中医药,2009,29(10):884-885.