基于SWATH技术筛选影响乌珠穆沁羊肉品质的蛋白
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摘要
为了研究影响乌珠穆沁羊肌肉品质的蛋白,通过IDA的方法对乌珠穆沁羊背最长肌与臀部肌肉进行蛋白全谱鉴定,1%假阳性条件下,共检测到蛋白质1003种。通过SWATH技术,差异倍数大于2或小于0.05作为阈值选取差异蛋白,获得背最长肌中高表达差异蛋白11个,其中7个与肌肉品质密切相关,这7个蛋白中Long-chain-fatty-acid-CoA ligase 1、Acyl-protein thioesterase 1、30Sribosomal protein S6、Sarcoplasmic/endoplasmic reticulum calcium ATPase 3通过影响脂肪沉积影响肌肉品质;Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1、Parvalbumin alpha通过影响几种氨基酸的变化,影响蛋白质组成,进而影响肌肉品质;Myosin-2heavy chain在背最长肌高表达,是决定肌纤维类型的重要蛋白,其通过影响肌纤维类型,决定肌肉品质。
In order to explore the protein which affects meat quality of Ujimqin sheep,in this study,the full spectrum identification of protein was done on longissimus muscle and hip muscles.The results show that 1003 proteins were detected under 1%false positive conditions.The protein differences of different fiber types were identified by SWATH technique.At the threshold of differential protein with a difference coefficient greater than 2 or less than 0.05,there were 11 differentially up-regulated proteins.7 of them are closely related to meat quality.The genes that affect the deposition of intramuscular fat deposit are Long-chain-fatty-acid--CoA ligase 1,Acyl-protein thioesterase 1,30 Sribosomal protein S6,Sarcoplasmic/endoplasmic reticulum calcium ATPase 3.Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 and Parvalbumin alpha play an important role in the negative regulation of tyrosine kinase,and then affect the quality of muscle which deter mines muscle quality.
In order to explore the protein which affects meat quality of Ujimqin sheep,in this study,the full spectrum identification of protein was done on longissimus muscle and hip muscles.The results show that 1003 proteins were detected under 1%false positive conditions.The protein differences of different fiber types were identified by SWATH technique.At the threshold of differential protein with a difference coefficient greater than 2 or less than 0.05,there were 11 differentially up-regulated proteins.7 of them are closely related to meat quality.The genes that affect the deposition of intramuscular fat deposit are Long-chain-fatty-acid--CoA ligase 1,Acyl-protein thioesterase 1,30 Sribosomal protein S6,Sarcoplasmic/endoplasmic reticulum calcium ATPase 3.Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 and Parvalbumin alpha play an important role in the negative regulation of tyrosine kinase,and then affect the quality of muscle which deter mines muscle quality.
引文
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[21]薛宝玲,张冬梅,王水清,等.乌珠穆沁羊背最长肌肌纤维类型的变化研究[J].内蒙古农业大学学报(自然科学版),2014,35(3):125-129.
[22]王水清.乌珠穆沁羊生长过程中肌纤维结构特性变化研究[D].呼和浩特:内蒙古农业大学,2012.
[23]王莉.牦牛肉肌纤维类型组成及其代谢酶活力差异对宰后肉嫩度的影响[D].兰州:甘肃农业大学,2016.
[24]吴菊清.猪肉肌原纤维蛋白乳化特性研究[D].南京:南京农业大学,2015.
[25] STEPHEN H,DEVOTO,ELLIE MELANON,et al.Identification of separate slow and fast muscle precursor cells in vivo,prior to somite formation.Development,1996,122:3371-3 380.
[2]刘露露,宋阳,苏丁丁.猪肌纤维发育及其对肉品质的影响[J].湖南畜牧兽医,2017(2):36-38.
[3]李威娜,黄勋和,陈洁波,等.五华三黄鸡及不同品种鸡肌纤维特性与肉品质的相关性[J].江苏农业科学,2017,45(2):157-160.
[4]郎玉苗,王勇峰,李敬,等.中国西门塔尔牛肌肉肌纤维类型和肉品质特性研究[J].中国畜牧兽医,2016,43(06):1 489-1 493.
[5] MI-SUNG K,JENS F,JOHN M A,et al.Protein Kinase D1Stimulates MEF2 Activity in Skeletal Muscle and Enhances Muscle Performance[J]. Molecular and Cellular Biology,2008,28(11):3 600-3 609.
[6]李伯江,李平华,吴望军,等.骨骼肌肌纤维形成机制的研究进展[J].中国农业科学,2014,47(6):1 200-1 207.
[7]薛宝玲.乌珠穆沁羊生长过程中肌纤维类型和MyHC基因变化分析[D].呼和浩特:内蒙古农业大学,2014.
[8]赵晓,莫德林,张悦,等.猪的骨骼肌生长发育研究进展[J].生命科学,2011,23(1):37-44.
[9]张哲奇,臧明伍,张凯华,等.国内外肉品品质变化机制机理研究进展[J].肉类研究,2017,31(2):57-63.
[10]陈康.高效液相色谱仪基本结构及使用[J].中国医疗设备,2009,24(1):37-39.
[11] ANDEY A,MANN M.Proteomics to study genes and genomics[J].Nature,2000,405(6788):837-846.
[12]李雯雯.SWATH采集模式蛋白质组学质谱应用新技术[J].中国食品,2011,581(13):49-49.
[13]李春波.创新的蛋白质组学质谱应用新技术--SWATH技术[J].现代科学仪器,2011(5):161-162.
[14]黄晓毅.生鲜猪肉品质变化的差异蛋白质组学研究[D].杭州:浙江工商大学,2010.
[15] XIE H,YANG D H,YAO H,et al.iTRAQ-based quantitative proteomic analysis reveals proteomic changes in leaves of cultivated tobacco(Nicotiana tabacum)in response to drought stress[J].Biochemical and Biophysical Research Communications,2016,469(3):768-775.
[16] WANG Z,SHANG P,LI Q,et al.iTRAQ-based proteomic analysis reveals key proteins affecting muscle growth and lipid deposition in pigs[J].Sci Rep,2017,7(1):46 717-46 727.
[17]李祎.HSF1和FACL4在原发性肝癌中的表达及意义[D].江苏苏州:苏州大学,2006.
[18]赵雅娟,苏琳,尹丽卿,等.蛋白质组学技术在肉品质中的研究进展[J].食品工业,2016(4):233-236.
[19] LAMETSCH R,KARLSSON A,ROSENVOLD K,et al.Postmortem proteome changes of porcine muscle related to tenderness[J].J Agric Food Chem,2003,51(24):6 992-6 997.
[20]汪以真.肌纤维多样性和可变性与肌肉生长和肉质的关系及其信号转导途径[C]∥中国畜牧兽医学会动物营养学分会.动物营养研究进展--中国畜牧兽医学会动物营养学分会第八届全国代表大学暨第十届学术研讨会论文集.中国畜牧兽医学会动物营养学分会,2008:7.
[21]薛宝玲,张冬梅,王水清,等.乌珠穆沁羊背最长肌肌纤维类型的变化研究[J].内蒙古农业大学学报(自然科学版),2014,35(3):125-129.
[22]王水清.乌珠穆沁羊生长过程中肌纤维结构特性变化研究[D].呼和浩特:内蒙古农业大学,2012.
[23]王莉.牦牛肉肌纤维类型组成及其代谢酶活力差异对宰后肉嫩度的影响[D].兰州:甘肃农业大学,2016.
[24]吴菊清.猪肉肌原纤维蛋白乳化特性研究[D].南京:南京农业大学,2015.
[25] STEPHEN H,DEVOTO,ELLIE MELANON,et al.Identification of separate slow and fast muscle precursor cells in vivo,prior to somite formation.Development,1996,122:3371-3 380.