乌饭树果实提取物抗氧化活性研究
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摘要
乌饭树是杜鹃花科越桔属的小浆果植物,有关乌饭树叶提取物抗氧化效果的研究报道较多,而对乌饭树果实抗氧化活性的研究报道却很少。为给其功能性品种的进一步选育和有效利用打下理论基础,对乌饭树果实中抗氧化物质的提取、含量及抗氧化活性等方面进行了研究。结果表明:乌饭果的水提取液清除DPPH自由基的效果为94.10%,其60%乙醇提取液的清除率为97.44%;乌饭叶的抗氧化物质(槲皮素)含量和清除DPPH自由基的效果都高于其果实;乌饭果的水提取液清除DPPH自由基的IC50值为0.32 mg·m L~(-1),当其清除率达到94.68%时所需水提液的浓度为2.08 mg·m L~(-1);乌饭果的水提取液清除50%DPPH自由基的用量是VC的97倍;乌饭树果实抗氧化物质(槲皮素)含量和水提取液抗氧化效果都呈现地理变化规律,较低纬度的赣南地区(南岭北坡)对选育优良品种具有重要意义。
Species Vaccinium bracteatum Thunb. that belongs to family Ericaceae genus Vaccinium is a kind of plant with small size of berry fruit, and relative data of antioxidant activity of extracts from leaves of Vaccinium bracteatum are common. However, there are limited modern data on antioxidant effects of extracts from fruits of this species, which is an exact objective of research in the present study. The results of this paper indicated that(1) rate of scavenging DPPH free radical is 94.68% by aqueous extracts from fruits, and 97.44% by 60% ethanol extracts from fruits;(2) contents of Quercetin in leaves is higher than in fruits, and the rate of scavenging DPPH free radical of aqueous extracts from leaves is also higher than from fruits;(3) the IC_(50) of aqueous extracts is 0.32 mg·m L~(-1), and concentration 2.08 mg·m L~(-1) of aqueous extracts is needed to reach the rate 94.68% of scavenging DPPH free radical;(4) the dosage of aqueous extracts from fruits is 97 times of Vc to scavenge 50% DPPH free radical;(5) both the content of Qercetin antioxidant and the effect of antioxidant of aqueous extracts from fruits of Vaccinium bracteatum berry fruits indicate geographical variance, which shows that it is important for us to find valuable genes of Vaccinium bracteatum in lower latitude regions of Nanling Mountains.
Species Vaccinium bracteatum Thunb. that belongs to family Ericaceae genus Vaccinium is a kind of plant with small size of berry fruit, and relative data of antioxidant activity of extracts from leaves of Vaccinium bracteatum are common. However, there are limited modern data on antioxidant effects of extracts from fruits of this species, which is an exact objective of research in the present study. The results of this paper indicated that(1) rate of scavenging DPPH free radical is 94.68% by aqueous extracts from fruits, and 97.44% by 60% ethanol extracts from fruits;(2) contents of Quercetin in leaves is higher than in fruits, and the rate of scavenging DPPH free radical of aqueous extracts from leaves is also higher than from fruits;(3) the IC_(50) of aqueous extracts is 0.32 mg·m L~(-1), and concentration 2.08 mg·m L~(-1) of aqueous extracts is needed to reach the rate 94.68% of scavenging DPPH free radical;(4) the dosage of aqueous extracts from fruits is 97 times of Vc to scavenge 50% DPPH free radical;(5) both the content of Qercetin antioxidant and the effect of antioxidant of aqueous extracts from fruits of Vaccinium bracteatum berry fruits indicate geographical variance, which shows that it is important for us to find valuable genes of Vaccinium bracteatum in lower latitude regions of Nanling Mountains.
引文
[1]Jian Zhang,Chun-Jun Chu,Xian-Lun Li,et al.Isolation and identification of antioxidant compounds in Vaccinium bracteatum Thunb.By UHPLG-Q-TOF LG/MS and their kidney damage protection[J].Journal of Functional Foods.2014,2:62-67
[2]房玉玲,秦明珠.乌饭树的研究进展[J].上海中医药杂志,2003,37(5):59-61.
[3]王静萍.越桔属植物果实的化学成分研究进展[J].植物学通报,1992,9(2):34-37.
[4]袁静,肖东.槲皮素抗肿瘤作用的研究进展[J].医药文摘,1996,18(5):3-6.
[5]刘清飞,朱爱兰,秦明珠.乌饭抗疲劳作用研究[J].时珍国医国药,1999,10(10):726-727.
[6]Li Wang,Yuan Xu,Sumei Zhou,et al.Interaction between Vaccinium bracteatum Thunb.leaf pigment and rice proteins.Food Chemistry[J].2016,194:272-278.
[7]王立,姚惠源,陶冠军,等.乌饭叶中黄酮类色素抗氧化活性[J].食品与生物技术学报,2006,25(4):81-84.
[8]王立,唐小舟,姚惠源,等.乌饭树树叶中黄酮类色素清除活性氧自由基的研究[J].食品科学,2005,26(12):98-102.
[9]魏国华,刘钟栋,许新德,等.乌饭叶提取物的抗氧化功能探讨[J].食品与发酵工业,2006,32(12):57-59.
[10]刘翼翔,吴永沛,陈俊,等.蓝莓不同多酚物质的分离与抑制细胞氧化损伤功能的比较[J].浙江大学学报(农业与生命科学版),2013,39(4):428-434.
[11]蔡凌云.乌饭树叶总黄酮体外抗氧化活性[J].中成药,2011,33(6):1054-1057.
[12]Li Wang,Xue Tong Zhang,Hai Yan Zhang,et al.Effect of Vaccinium bracteatum Thunb.leaves extract on blood glucose and plasma lipid levels in streptozotocin-induced diabetic mice[J].Journal of Ethnopharmacology,2010,130:465-469
[13]凌关庭.抗氧化食品与健康[M].北京:化学工业出版社,2004.
[14]唐传核.植物生物活性物质[M].北京:化学工业出版社,2005.
[15]Wonhwa Lee,Jong-Sup Bae.Antithrombotic and antiplatelet activities of orientin in vitro and in vivo[J].Journal of Functional Foods,2015,17:388-398
[16]Haifeng Qian,Yan Li,Li Wang.Vaccinium bracteatum Thunb.Leaves’polysaccharide alleviates hepatic gluconeogenesis visa the downregulation of mi R-137[J].Biomedcine and pharmacotherap,2017,95:1397-1403.
[17]王立,张雪彤,姚惠源.乌饭树树叶及其提取物对视网膜光损伤的保护作用[J].西安交通大学学报(医学版),2006,27(3):284-303.
[18]刘知远,陈福星.HPLC法测定乌饭树叶活性成分槲皮素的含量[J].中国药师,2012,15(10):1507-1508.
[19]胡移辉.乌饭的营养成分测定[J].南京农业大学学报,1993,16(2):118-119.
[20]刘仁林,胡明娇,李江,等.乌饭果实大小的地理变异研究[J].经济林研究,2016,34(3):114-120.
[21]庞伟,宋纪蔷,徐抗震,等.苹果多酚铝镁盐沉淀的特性及自由基清除研究[J].食品科学,2007,28(6):36-38.
[22]马涛,杨容,李越敏,等.苹果多酚提取物抗氧化活性研究[J].食品研究与开发,2008,29(12):189-192.
[23]杨振东,蒋彦婕.乌饭树叶提取物抗氧化活性研究及组分分析[J].食品与发酵工业,2015,41(9):144-147.
[24]唐柯,王梓萱,张伟宏,等.不同品种蓝莓分类物质组成特征及抗氧化能力比较分析[J].食品与发酵工业,2017,43(8):103-107.
[25]包怡红,梁敏,赵行,等.俄罗斯野生蓝莓引种前后营养成分含量及抗氧化活性的比较分析[J].中国酿造,2017,36(5):156-160.
[2]房玉玲,秦明珠.乌饭树的研究进展[J].上海中医药杂志,2003,37(5):59-61.
[3]王静萍.越桔属植物果实的化学成分研究进展[J].植物学通报,1992,9(2):34-37.
[4]袁静,肖东.槲皮素抗肿瘤作用的研究进展[J].医药文摘,1996,18(5):3-6.
[5]刘清飞,朱爱兰,秦明珠.乌饭抗疲劳作用研究[J].时珍国医国药,1999,10(10):726-727.
[6]Li Wang,Yuan Xu,Sumei Zhou,et al.Interaction between Vaccinium bracteatum Thunb.leaf pigment and rice proteins.Food Chemistry[J].2016,194:272-278.
[7]王立,姚惠源,陶冠军,等.乌饭叶中黄酮类色素抗氧化活性[J].食品与生物技术学报,2006,25(4):81-84.
[8]王立,唐小舟,姚惠源,等.乌饭树树叶中黄酮类色素清除活性氧自由基的研究[J].食品科学,2005,26(12):98-102.
[9]魏国华,刘钟栋,许新德,等.乌饭叶提取物的抗氧化功能探讨[J].食品与发酵工业,2006,32(12):57-59.
[10]刘翼翔,吴永沛,陈俊,等.蓝莓不同多酚物质的分离与抑制细胞氧化损伤功能的比较[J].浙江大学学报(农业与生命科学版),2013,39(4):428-434.
[11]蔡凌云.乌饭树叶总黄酮体外抗氧化活性[J].中成药,2011,33(6):1054-1057.
[12]Li Wang,Xue Tong Zhang,Hai Yan Zhang,et al.Effect of Vaccinium bracteatum Thunb.leaves extract on blood glucose and plasma lipid levels in streptozotocin-induced diabetic mice[J].Journal of Ethnopharmacology,2010,130:465-469
[13]凌关庭.抗氧化食品与健康[M].北京:化学工业出版社,2004.
[14]唐传核.植物生物活性物质[M].北京:化学工业出版社,2005.
[15]Wonhwa Lee,Jong-Sup Bae.Antithrombotic and antiplatelet activities of orientin in vitro and in vivo[J].Journal of Functional Foods,2015,17:388-398
[16]Haifeng Qian,Yan Li,Li Wang.Vaccinium bracteatum Thunb.Leaves’polysaccharide alleviates hepatic gluconeogenesis visa the downregulation of mi R-137[J].Biomedcine and pharmacotherap,2017,95:1397-1403.
[17]王立,张雪彤,姚惠源.乌饭树树叶及其提取物对视网膜光损伤的保护作用[J].西安交通大学学报(医学版),2006,27(3):284-303.
[18]刘知远,陈福星.HPLC法测定乌饭树叶活性成分槲皮素的含量[J].中国药师,2012,15(10):1507-1508.
[19]胡移辉.乌饭的营养成分测定[J].南京农业大学学报,1993,16(2):118-119.
[20]刘仁林,胡明娇,李江,等.乌饭果实大小的地理变异研究[J].经济林研究,2016,34(3):114-120.
[21]庞伟,宋纪蔷,徐抗震,等.苹果多酚铝镁盐沉淀的特性及自由基清除研究[J].食品科学,2007,28(6):36-38.
[22]马涛,杨容,李越敏,等.苹果多酚提取物抗氧化活性研究[J].食品研究与开发,2008,29(12):189-192.
[23]杨振东,蒋彦婕.乌饭树叶提取物抗氧化活性研究及组分分析[J].食品与发酵工业,2015,41(9):144-147.
[24]唐柯,王梓萱,张伟宏,等.不同品种蓝莓分类物质组成特征及抗氧化能力比较分析[J].食品与发酵工业,2017,43(8):103-107.
[25]包怡红,梁敏,赵行,等.俄罗斯野生蓝莓引种前后营养成分含量及抗氧化活性的比较分析[J].中国酿造,2017,36(5):156-160.