红松松针精油脂肪酸GC-MS分析
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摘要
用索氏提取器提取红松松针精油,将提取物的油脂甲脂化后利用气相色谱-质谱联用技术(GC-MS)进行分离和鉴定.结果表明:红松松针精油含有丰富的饱和脂肪酸和不饱和脂肪酸,饱和脂肪酸质量分数占总脂肪酸质量分数的74.32%,不饱和脂肪酸占25.68%;鉴定出31种脂肪酸,相对质量分数比较高的是棕榈酸(26.86%)、月桂酸(25.05%)、肉豆蔻酸(6.33%)、芥子酸(5.98%)、二十二碳六烯酸(DHA)(5.4%)、硬脂酸(4.38%),γ-亚麻酸和亚油酸分别是1.55%和2.18%,α-亚麻酸未检出.
Using Soxhlet extractor to extract essential oil of Pinus koraiensis needles,adopting gas chromatography-mass spectrometry(GC-MS) to conduct separation and detection after the extractive was methylated.The results showed that the essential oil of Pinus koraiensis needles was rich in saturated fatty acid and unsaturated fatty acid. The content of saturated fatty acids accounted for 74. 32% of total fatty acids. The content of unsaturated fatty acids was 25. 68% of total fatty acids. 31 fatty acids were detected. Relatively high contents among them,palmitic acid is 26.86%,lauric acid is 25.05%,myristic acid is 6.33%,sinapic acid is 5.98%,docosahexaenoic acid(DHA) is 5.4%,stearic acid is 4.38%,γ-linolenic acid is 1.55%,linoleic acid is 2.18%,α-linolenic acid was not detected.
Using Soxhlet extractor to extract essential oil of Pinus koraiensis needles,adopting gas chromatography-mass spectrometry(GC-MS) to conduct separation and detection after the extractive was methylated.The results showed that the essential oil of Pinus koraiensis needles was rich in saturated fatty acid and unsaturated fatty acid. The content of saturated fatty acids accounted for 74. 32% of total fatty acids. The content of unsaturated fatty acids was 25. 68% of total fatty acids. 31 fatty acids were detected. Relatively high contents among them,palmitic acid is 26.86%,lauric acid is 25.05%,myristic acid is 6.33%,sinapic acid is 5.98%,docosahexaenoic acid(DHA) is 5.4%,stearic acid is 4.38%,γ-linolenic acid is 1.55%,linoleic acid is 2.18%,α-linolenic acid was not detected.
引文
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[16]张泽生,高山,郭擎,等.棕榈油酸的研究现状及展望[J].中国食品添加剂,2016(9):198-202.
[17]宋波.月桂酸的性质、制备及应用[J].精细化工信息,1987(1):1-5.
[18]仇记红,侯利霞.松子油加工工艺及脂肪酸组成研究进展[J].粮食与油脂,2018,31(12):10-12.
[19]刘晓荻.世界卫生组织促全球5年内杜绝反式脂肪酸[J].基础医学与临床,2019,39(1):156.
[20]陈伟,孙秀霞,刘伟伟,等.不同树种松花粉脂肪酸的气相色谱-质谱联用分析比较[J].食品科学,2012,33(14):219-222.
[2]崔和平,郭兴凤.多不饱和脂肪酸对人体神经系统保健作用研究进展[J].河南工业大学学报(自然科学版),2012,33(3):97-102.
[3]周斌,彭淑牖,牟一平.茶油对梗阻性黄疸肝脏保护作用的实验研究[J].温州医学院学报,2000,30(3):193-195.
[4]马颀.糖尿病视网膜病变的脂肪酸营养[D].杭州:浙江大学,2015.
[5]黄丽群.园林植物叶片不饱和脂肪酸与抗寒性关系的研究[D].长沙:中南林学院,2005.
[6]马忠孝,李奉,郭森,等.水曲柳种子脂肪酸GC-MS分析[J].西北大学学报(自然科学版),2018,48(4):540-544.
[7]刘辉,石丽华,张健,等.破壁马尾松花粉中脂肪酸的成分分析[J].中国野生植物资源,2006,25(5):57-58.
[8]牛之瑞.红松仁多不饱和脂肪酸纯化及功能研究[D].哈尔滨:东北林业大学,2008.
[9]朱雪梅,阮霞,胡蒋宁,等.松籽油脂肪酸组成及分布特征分析[J].食品工业科技,2012,33(10):65-68.
[10]王振宇,牛之瑞.红松仁不饱和脂肪酸对肥胖大鼠肝脏脂肪代谢的影响[J].营养学报,2008,30(6):547-550.
[11]陈秀丽.松仁油对脂代谢的影响及抗氧化功能研究[D].哈尔滨:东北林业大学,2008.
[12] Anne M,Colette S R,Daniel G,et al.Effects of dietary alpha linolenic acid on cholesterol metabolism in male and female hamsters of the LPN strain[J].Journal of Nutritional Biochemistry,2004,15(1):51-61.
[13] Berry E M,Hirseh J.Does dietary linolenic acid influence blood pressure[J].The American Journal of Clinical Nutrition,1986,44(3):336-340.
[14] Kato T,Hancock R L,Mohammadpour H,et al.Influence of omega3 fatty acids on the growth of human colon carcinoma in nude mice[J].Cancer Lett,2002,187(1-2):169-177.
[15]曹景玉,吴力群,郭卫东,等.ω-3多不饱和脂肪酸对肝癌细胞生长抑制的作用及机制[J].中国普通外科杂志,2009,18(2):150-155.
[16]张泽生,高山,郭擎,等.棕榈油酸的研究现状及展望[J].中国食品添加剂,2016(9):198-202.
[17]宋波.月桂酸的性质、制备及应用[J].精细化工信息,1987(1):1-5.
[18]仇记红,侯利霞.松子油加工工艺及脂肪酸组成研究进展[J].粮食与油脂,2018,31(12):10-12.
[19]刘晓荻.世界卫生组织促全球5年内杜绝反式脂肪酸[J].基础医学与临床,2019,39(1):156.
[20]陈伟,孙秀霞,刘伟伟,等.不同树种松花粉脂肪酸的气相色谱-质谱联用分析比较[J].食品科学,2012,33(14):219-222.