摘要
为了解貂源铜绿假单胞菌流行株基本特性,对分离的20株铜绿假单胞菌进行药物敏感性试验、血清型鉴定、ToxA基因检测、强毒株筛选及绝对致死量(LD_(100))测定。结果表明,铜绿假单胞菌分离株耐药现象比较严重,对氨苄西林、头孢呋辛、头孢曲松、头孢噻吩、头孢噻肟、头孢西丁、头孢唑林和四环素不敏感,对妥布霉素敏感率为95%;铜绿假单胞菌分离株的血清型为G型和B型,分别占85%和15%;20株铜绿假单胞菌均能检测出毒素基因ToxA;小鼠致死性试验表明,铜绿假单胞菌分离株之间毒力差异很大,少数菌株如ZHDL9、ZHDL6等均表现出较强的毒力。以菌株ZHDL9为代表株建立小鼠鼻腔感染模型,采用建立的感染模型测定得菌株ZHDL9对小鼠的LD_(100)为5×10~7 cfu。由此可见,分离的20株铜绿假单胞菌对临床常用抗生素耐药严重,对妥布霉素的敏感率为95%,提示该种抗生素可用于临床治疗水貂出血性肺炎,而菌株ZHDL9可作为生物制品研发的候选菌株。
To study the basic characteristics for epidemic strains of Pseudomonas aeruginosa isolated from mink,20 strains of Pseudomonas aeruginosa were tested for drug sensitivity,serotype identification,ToxA gene detection,screening of virulent strains and LD_(100) determination.The results showed that the isolates were resistant to ampicillin,cefuroxime,ceftriaxone,cefothiophene,cefotaxime,cefoxitin,cefazolin and tetracycline,and the susceptibility rate to tobramycin was 95%.The sera of the isolates were G-type and B-type,and the proportion was 85% and 15% respectively.ToxA gene could be detected in the isolates;the lethal test in mice showed that isolated strains appeared difference in virulence,a few strains such as ZHDL9,ZHDL6 showed a stronger virulence.Then we established the nasal infection in mice model with ZHDL9,and the absolutely lethal dose of ZHDL9 was 5×10~7 cfu.It is concluded that the 20 Pseudomonas aenginosa isolates are highly resistant to commonly used antibiotics,and the susceptibility rate to tobramycin was 95%,suggesting that this antibiotic can be used for clinical treatment for mink hemorrhagic pneumonia.Meanwhile,strain ZHDL9 can be used as a candidate strain for developing biological products.
引文
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