系列玉米雄穗突变系的创制
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摘要
通过对野生型玉米自交系(A378)离体花粉生殖细胞N-甲基-N亚硝基脲处理所得M1～M2群体的雄穗性状的筛查,创制出3种类型的玉米雄穗突变系。结果显示,与A378相比,第1类型雄穗突变系具有无分枝、主轴发育不良、无花粉的性状;第2类型雄穗突变系显示主轴退化、主轴与分枝上部分小穗雌化的性状;第3类型雄穗突变系具有正常发育的主轴与短小簇生的分枝。这些雄穗突变性状在M2群体中均出现表型分离现象。其中,1份雄穗雌化突变系具有显性性状,别的突变系均具有隐性性状。这些玉米突变系有望用于玉米雄性生殖器官发育与性状表达遗传机制的研究。
In this study, three types of tassel mutant lines of maize were created, screening the tassel characters in M1-M2 progeny populations obtained by the N-methyl-N-nitrosourea treatment of pollen germ cells of the wild-type maize inbred line, A378. The results showed that compared with A378, the type-I tassel mutants had the characters of no-branches, principal-axis dysplasia and no-pollen.The type-II tassel mutants showed the characters of principal-axis degeneration, feminization of principal-axis and partial spikelets on branch. The type-III tassel mutants had the normally developed principal-axis and short cluster branches. These mutant characters of tassel exhibited phenotypic segregation in the M2 population. Among them, one line with tassel feminization was dominant mutant, and the other lines all were recessive mutants. These maize mutant lines created in this study is expected to be used in studies for the genetic mechanisms concerning character expression and development of male reproductive organs in maize.
In this study, three types of tassel mutant lines of maize were created, screening the tassel characters in M1-M2 progeny populations obtained by the N-methyl-N-nitrosourea treatment of pollen germ cells of the wild-type maize inbred line, A378. The results showed that compared with A378, the type-I tassel mutants had the characters of no-branches, principal-axis dysplasia and no-pollen.The type-II tassel mutants showed the characters of principal-axis degeneration, feminization of principal-axis and partial spikelets on branch. The type-III tassel mutants had the normally developed principal-axis and short cluster branches. These mutant characters of tassel exhibited phenotypic segregation in the M2 population. Among them, one line with tassel feminization was dominant mutant, and the other lines all were recessive mutants. These maize mutant lines created in this study is expected to be used in studies for the genetic mechanisms concerning character expression and development of male reproductive organs in maize.
引文
[1]SATOH H,MATSUSAKA H,KUMAMARU T. Use of N-methyl-Nnitrosourea treatment of fertilized egg cells for saturation mutagenesis of rice[J]. Breeding Science,2010,60(5):475-485.
[2]牛鹏飞,李珍,马海林,等.系列水性环境体系中玉米离体花粉的活力解析[J].山西农业科学,2018,46(5):722-724,728.
[3]岑慧慧,田承华,程庆军,等.不同N-甲基-N-亚硝基脲处理下高粱离体花粉的活力解析[J].山西农业科学,2018,46(5):725-728.
[4]徐洪文,宋凤斌,童淑媛.玉米雄穗发生发展规律及保护酶活性研究[J].华北农学报,2011,26(6):141-144.
[5]岳玉兰,朱敏,于雷,等.玉米雄穗对产量影响研究进展[J].玉米科学,2010,18(4):150-152.
[6] SHENG C,REN J,YAN D,et al. Research progress on differentiation and development of tassel in maize[J]. Crop Research,2014,119(33):6478-6481.
[7] ABRECHT D G,CARBERRY P S. The influence of water deficit prior to tassel initiation on maize growth,development and yield[J].Field Crops Research,1993,31(1):55-69.
[8]TIMOFEJEVA L,SKIBBE D S,LEE S,et al. Cytological characterization and allelism testing of anther developmental mutants identified in a screen of maize male dterile lines[J]. Genes,2013,3(2):231-249.
[9] DUVICK D N. Cytoplasmic pollen sterility in corn[J]. Advances in Genetics,1965,13:1-56.
[10]WANG D,SKIBBE D S,WALBOT V. Maize Male sterile 8(Ms8),a putativeβ-1,3-galactosyltransferase,modulates cell division,expansion,and differentiation during early maize anther development[J]. Plant Reprod,2013,26(4):329-338.
[11] CHAUBAL R,ANDERSON J R,TRIMNELL M R,et al. The transformation of anthers in the msca1 mutant of maize[J]. Planta,2003,216(5):778-788.
[12] VERNOUD V,LAIGLE G,ROZIER F,et al. The HD-ZIP IV transcription factor OCL4 is necessary for trichome patterning and anther development in maize[J]. Plant Journal,2009,59(6):883-894.
[13]WANG C J,NAN G L,KELLIHER T,et al. Maize multiple archesporial cells 1(mac1),an ortholog of rice TDL1A,modulates cell proliferation and identity in early anther development[J]. Development,2012,139(14):2594-2603.
[14]崔贵梅,孙毅,郝曜山,等.玉米花粉体外萌发方法改进及其对花粉介导转基因的作用[J].植物学报,2012,47(2):155-161.
[15]MOON J,SKIBBE D,TIMOFEJEVA L,et al. Regulation of cell divisions and differentiation by male sterility 32 is required for anther development in maize[J]. Plant Journal,2013,76(4):592-602.
[16] XING A,GAO Y,YE L,et al. A rare SNP mutation in Brachytic2moderately reduces plant height and increases yield potential in maize[J]. Journal of Experimental Botany,2015,66(13):3791-3802.
[17] CIGAN A M,UNGER E,XU R J,et al. Phenotypic complementation of ms45 maize requires tapetal expression of MS45[J]. Sexual Plant Reproduction,2001,14(3):135-142.
[18]MURAI K,TAKUMI S,KOGA H,et al. Pistillody,homeotic transformation of stamens into pistil-like structures,caused by nuclear-cytoplasm interaction in wheat[J]. Plant Journal,2002,29(2):13.
[19] LEINO M,TEIXEIRA R,LANDGREN M,et al. Brassica napus lines with rearranged Arabidopsis mitochondria display CMS and a range of developmental aberrations[J]. Theoretical and Applied Genetics,2003,106:1156-1163.
[20] MALIK M,VYAS P,RANGASWAMY N S,et al. Development of two new cytoplasmic male-sterile lines in Brassica juncea through wide hybridization[J]. Plant Breeding,1999,118:75-78.
[21] LINKE B,NOTHNAGEL T,B魻RNER T. Flower development in carrot CMS plants:mitochondria affect the expression of MADS box genes homologous to GLOBOSA and DEFICIENS[J]. Plant Journal,2003,34(1):11.
[22] PHILLIPS K A,SKIRPAN A L,LIU X,et al. Vanishing tassel2encodes a grass-specific tryptophan aminotransferase required for vegetative and reproductive development in maize[J]. Plant Cell,2011,23(2):550-566.
[23]丁帅涛,罗红兵,程晓梅,等.光温敏无雄穗系玉米I17雄穗分化发育研究[J].玉米科学,2015,23(3):21-27.
[2]牛鹏飞,李珍,马海林,等.系列水性环境体系中玉米离体花粉的活力解析[J].山西农业科学,2018,46(5):722-724,728.
[3]岑慧慧,田承华,程庆军,等.不同N-甲基-N-亚硝基脲处理下高粱离体花粉的活力解析[J].山西农业科学,2018,46(5):725-728.
[4]徐洪文,宋凤斌,童淑媛.玉米雄穗发生发展规律及保护酶活性研究[J].华北农学报,2011,26(6):141-144.
[5]岳玉兰,朱敏,于雷,等.玉米雄穗对产量影响研究进展[J].玉米科学,2010,18(4):150-152.
[6] SHENG C,REN J,YAN D,et al. Research progress on differentiation and development of tassel in maize[J]. Crop Research,2014,119(33):6478-6481.
[7] ABRECHT D G,CARBERRY P S. The influence of water deficit prior to tassel initiation on maize growth,development and yield[J].Field Crops Research,1993,31(1):55-69.
[8]TIMOFEJEVA L,SKIBBE D S,LEE S,et al. Cytological characterization and allelism testing of anther developmental mutants identified in a screen of maize male dterile lines[J]. Genes,2013,3(2):231-249.
[9] DUVICK D N. Cytoplasmic pollen sterility in corn[J]. Advances in Genetics,1965,13:1-56.
[10]WANG D,SKIBBE D S,WALBOT V. Maize Male sterile 8(Ms8),a putativeβ-1,3-galactosyltransferase,modulates cell division,expansion,and differentiation during early maize anther development[J]. Plant Reprod,2013,26(4):329-338.
[11] CHAUBAL R,ANDERSON J R,TRIMNELL M R,et al. The transformation of anthers in the msca1 mutant of maize[J]. Planta,2003,216(5):778-788.
[12] VERNOUD V,LAIGLE G,ROZIER F,et al. The HD-ZIP IV transcription factor OCL4 is necessary for trichome patterning and anther development in maize[J]. Plant Journal,2009,59(6):883-894.
[13]WANG C J,NAN G L,KELLIHER T,et al. Maize multiple archesporial cells 1(mac1),an ortholog of rice TDL1A,modulates cell proliferation and identity in early anther development[J]. Development,2012,139(14):2594-2603.
[14]崔贵梅,孙毅,郝曜山,等.玉米花粉体外萌发方法改进及其对花粉介导转基因的作用[J].植物学报,2012,47(2):155-161.
[15]MOON J,SKIBBE D,TIMOFEJEVA L,et al. Regulation of cell divisions and differentiation by male sterility 32 is required for anther development in maize[J]. Plant Journal,2013,76(4):592-602.
[16] XING A,GAO Y,YE L,et al. A rare SNP mutation in Brachytic2moderately reduces plant height and increases yield potential in maize[J]. Journal of Experimental Botany,2015,66(13):3791-3802.
[17] CIGAN A M,UNGER E,XU R J,et al. Phenotypic complementation of ms45 maize requires tapetal expression of MS45[J]. Sexual Plant Reproduction,2001,14(3):135-142.
[18]MURAI K,TAKUMI S,KOGA H,et al. Pistillody,homeotic transformation of stamens into pistil-like structures,caused by nuclear-cytoplasm interaction in wheat[J]. Plant Journal,2002,29(2):13.
[19] LEINO M,TEIXEIRA R,LANDGREN M,et al. Brassica napus lines with rearranged Arabidopsis mitochondria display CMS and a range of developmental aberrations[J]. Theoretical and Applied Genetics,2003,106:1156-1163.
[20] MALIK M,VYAS P,RANGASWAMY N S,et al. Development of two new cytoplasmic male-sterile lines in Brassica juncea through wide hybridization[J]. Plant Breeding,1999,118:75-78.
[21] LINKE B,NOTHNAGEL T,B魻RNER T. Flower development in carrot CMS plants:mitochondria affect the expression of MADS box genes homologous to GLOBOSA and DEFICIENS[J]. Plant Journal,2003,34(1):11.
[22] PHILLIPS K A,SKIRPAN A L,LIU X,et al. Vanishing tassel2encodes a grass-specific tryptophan aminotransferase required for vegetative and reproductive development in maize[J]. Plant Cell,2011,23(2):550-566.
[23]丁帅涛,罗红兵,程晓梅,等.光温敏无雄穗系玉米I17雄穗分化发育研究[J].玉米科学,2015,23(3):21-27.