新疆多民族食管癌患者癌组织中p53蛋白表达及其临床意义
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摘要
目的探讨新疆多民族食管癌患者癌组织及癌旁组织中p53蛋白表达情况及其临床意义。方法采用免疫组织化学En Vision法检测214例维吾尔族、汉族、哈萨克族食管癌患者癌组织以及100例癌旁正常组织中p53蛋白表达情况,并结合不同民族、肿瘤分化程度等临床病理特点进行分析。结果 44.3%的食管癌病例检测到p53蛋白的阳性表达,p53蛋白在癌组织的阳性表达率高于癌旁正常组织,差异有统计学意义(P<0.05)。维吾尔族、汉族、哈萨克族食管癌组织中p53蛋白阳性表达率分别为45.6%、38.4%、49.3%,各民族间差异无统计学意义(P>0.05),p53在不同性别、分化程度和淋巴结转移之间的阳性表达差异有统计学意义(P<0.05)。患者的预后与淋巴结转移、脉管侵犯、浸润程度状况有关(P<0.05)。结论 p53基因在新疆不同民族食管癌的发生、发展及淋巴结转移中可能有重要作用,可作为预测转移潜能的参考指标。
Objective To study the expression of p53 gene and its clinical significance in esophageal squamous cell carcinoma( ESCC) and adjacent normal tissues of Multi-ethnic groups in Xinjiang. Methods Immunohistochemistry Envision method was used to detect the p53 gene expression of 214 cases with ESCC and 100 cases of adjacent normal tissues,including Uyghur,Han,Kazak three different ethnic groups. And the differences of clinicopathological characteristics among them were analyzed. Results p53 protein positively expressed in 44. 3 % cases of ESCC. The positive rate of p53 protein expression in ESCC tissues and adjacent normal tissues was significantly different( P < 0. 05). The positive rate of p53 protein expression in Uyghur,Han,Kazak ESCC patients was 45. 6 %,38. 4 %,49. 3 %,respectively. There was no significant differences among the three different ethnic groups( P >0. 05). However,the positive expression of p53 protein had statistical differences among genders,degrees of tumor differentiation and lymph node metastasis( P < 0. 05). After analyzing the correlation between clinical pathologicalfactors and prognosis,we found that the prognosis of patients had significant differences according to lymph node metastasis,vascular invasion and depth of tumor invasion( P < 0. 05). Conclusion p53 gene may play an important role in the occurrence and development of esophageal carcinoma and lymph node metastasis in different ethnic groups in Xinjiang,and it can be used as a reference index for predicting the metastatic potential of ESCC.
Objective To study the expression of p53 gene and its clinical significance in esophageal squamous cell carcinoma( ESCC) and adjacent normal tissues of Multi-ethnic groups in Xinjiang. Methods Immunohistochemistry Envision method was used to detect the p53 gene expression of 214 cases with ESCC and 100 cases of adjacent normal tissues,including Uyghur,Han,Kazak three different ethnic groups. And the differences of clinicopathological characteristics among them were analyzed. Results p53 protein positively expressed in 44. 3 % cases of ESCC. The positive rate of p53 protein expression in ESCC tissues and adjacent normal tissues was significantly different( P < 0. 05). The positive rate of p53 protein expression in Uyghur,Han,Kazak ESCC patients was 45. 6 %,38. 4 %,49. 3 %,respectively. There was no significant differences among the three different ethnic groups( P >0. 05). However,the positive expression of p53 protein had statistical differences among genders,degrees of tumor differentiation and lymph node metastasis( P < 0. 05). After analyzing the correlation between clinical pathologicalfactors and prognosis,we found that the prognosis of patients had significant differences according to lymph node metastasis,vascular invasion and depth of tumor invasion( P < 0. 05). Conclusion p53 gene may play an important role in the occurrence and development of esophageal carcinoma and lymph node metastasis in different ethnic groups in Xinjiang,and it can be used as a reference index for predicting the metastatic potential of ESCC.
引文
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[15]袁嘉敏,凌志强,毛伟敏.p53在食管癌中的研究进展[J].国际消化病杂志,2012,32(2):90-92.
[16]孙海涛,高宏,张艺,等.p53、PCNA及P-gp在食管癌中表达的研究[J].航空航天医学杂志,2012,23(1):35-37.
[2]Li Y,An J,Huang S,et al.PLCE1 suppresses p53 expression in esophageal cancer cells[J].Cancer Invest,2014,32(6):236-240.
[3]Xu Y,Yu X,Chen Q,et al.Neoadjuvant versus adjuvant treatment:which one is better for resectable esophageal squamous cell carcinoma?[J].World J Surg Oncol,2012,10(2):1-8.
[4]张思维,张敏,李光琳,等.2003~2007年中国食管癌发病与死亡分析[J].中国肿瘤,2012,21(4):241-247.
[5]王慧,李梦妍,袁媛,等.172例新疆哈萨克族食管鳞状细胞癌临床病理资料分析[J].现代肿瘤医学,2016(4):562-565.
[6]Rice TW,Blackstone EH,Rusch VW.7th Edition of the AJCC cancer staging manual:esophagus and esophagogastric junction[J].Ann Surg Oncol,2010,17(7):1721-4.
[7]Fagundes RB,Melo CR,Pütten ACK,et al.p53 immunoexpression:an aid to conventional methods in the screening of precursor lesions of squamous esophageal cancer in patients at high-risk?[J].Cancer Detect Prev,2005,29(3):227-32.
[8]钟亚莉,刘超,赵治国.p53、MDR-1基因在中老年食管癌患者中的表达及临床运用分析[J].转化医学电子杂志,2016,3(1):12-14.
[9]占向鸿,张晓膺,狄冬梅,等.65例食管癌患者的nm23、p53、PCNA的表达及其临床预后意义[J].苏州大学学报:医学版,2006,26(5):830-831.
[10]杜日昌,陈玉英,谭丽珊,等.食管癌中Caveolin-1与p53的表达及临床意义[J].中外医学研究,2011,9(14):7-10.
[11]李翠翠,李苏宜.食管癌突变型p53过表达临床研究新进展[J].癌症进展,2012,10(3):225-227.
[12]费素娟,黄水平,周力新,等.食管癌组织中COX-2、p53和PCNA的表达及意义[J].中国癌症杂志,2003,13(1):17-20.
[13]王根进,胡建军.食管癌组织中p53基因蛋白过度表达及其临床意义[J].华中医学杂志,2003,27(1):18-19.
[14]陶仪声,武世伍,宗永生.抑癌基因p53在不同地区食管癌中的表达及临床预后分析[J].肿瘤学杂志,2002,8(2):79-79.
[15]袁嘉敏,凌志强,毛伟敏.p53在食管癌中的研究进展[J].国际消化病杂志,2012,32(2):90-92.
[16]孙海涛,高宏,张艺,等.p53、PCNA及P-gp在食管癌中表达的研究[J].航空航天医学杂志,2012,23(1):35-37.