ACE基因I/D多态性与异常体液型2型糖尿病的关联研究
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摘要
目的:探讨血管紧张素转换酶(ACE)基因16内含子插入/缺失(I/D)多态性与新疆维吾尔自治区汉族人群异常体液型2型糖尿病(type 2 diabetes mellitus,T2DM)的关系。方法:用维吾尔医学和分子生物学技术互相结合的方法,分析87例T2DM患者和157例正常人ACE基因16内含子I/D多态性。结果:(1)异常粘液质型T2DM组II基因型的频率较对照组显著增高(20.00%比4.43%,p=0.023<0.05)。(2)在异常粘液质型T2DM组中携带ID、DD基因型的个体较携带II基因型的个体患T2DM的危险性增加(OR=5.618,95%CI为1.451-21.739;OR=4.386,95%CI为0.785-24.390)。结论:ACE基因16内含子插入/缺失(I/D)多态性与新疆汉族人异常粘液质型T2DM的危险性相关,与新疆汉族人异常胆液质型、异常黑胆质型和异常血液质型T2DM的危险性无关。
Objective: To study the I / D polymorphism of ACE gene and it's susceptibility to type 2 diabetes( T2DM) in chinese Han population of Xinjiang. Method: Using the combined methods of Uighur medicine theory and molecular biology techniques to detect the I / D polymorphism at the intron 16 of the ACE gene in 87 T2 DM patients and 157 controls. Result:( 1) The frequency of the Ⅱ genotype for patients with abnormal Balgham Hilit was significantly higher than these for controls( 20. 00% vs 4. 43%,p = 0. 023 < 0. 05).( 2) Compared with II genotype,the susceptibility to T2 DM with the ID,DD genotypes were increased by 5. 618( OR = 5. 618,95% CI 为 1. 451-21. 739) and 4. 386( OR = 4. 386,95% CI 为 0. 785- 24. 390) in T2 DM patients with abnormal Balgham Hilit. Conclusion: These data suggest that the polymorphism of I / D of ACE gene have a susceptibility to T2 DM risk in patients of chinese Han population which carried with abnormal Balgham Hilit,and it is not associated with T2 DM risk in patients with uighur medicine abnormal Savda Hilit,uighur medicine abnormal Sapra Hilit and uighur medicine abnormal Qan Hilit.
Objective: To study the I / D polymorphism of ACE gene and it's susceptibility to type 2 diabetes( T2DM) in chinese Han population of Xinjiang. Method: Using the combined methods of Uighur medicine theory and molecular biology techniques to detect the I / D polymorphism at the intron 16 of the ACE gene in 87 T2 DM patients and 157 controls. Result:( 1) The frequency of the Ⅱ genotype for patients with abnormal Balgham Hilit was significantly higher than these for controls( 20. 00% vs 4. 43%,p = 0. 023 < 0. 05).( 2) Compared with II genotype,the susceptibility to T2 DM with the ID,DD genotypes were increased by 5. 618( OR = 5. 618,95% CI 为 1. 451-21. 739) and 4. 386( OR = 4. 386,95% CI 为 0. 785- 24. 390) in T2 DM patients with abnormal Balgham Hilit. Conclusion: These data suggest that the polymorphism of I / D of ACE gene have a susceptibility to T2 DM risk in patients of chinese Han population which carried with abnormal Balgham Hilit,and it is not associated with T2 DM risk in patients with uighur medicine abnormal Savda Hilit,uighur medicine abnormal Sapra Hilit and uighur medicine abnormal Qan Hilit.
引文
[1]马合木提江·艾孜木,古丽斯阿依·热合木都拉.关于维吾尔医药学与基因科学相结合治疗癌症的初步探索[J].中国民族医药杂志,2006,(4):22-23.
[2]周青.2型糖尿病的相关基因多态性的研究进展[J].铜陵职业技术学院学报,2008,(1):49-52.
[3]郭明秋.ACE基因和AT1R基因多态性与糖尿病相关性的研究[D].青岛:青岛大学,2006.
[4]黄青阳,程孟荣,姬森林.2型糖尿病易感基因的连锁和关联研究(英文)[J].遗传学报,2006,(7):3-19.
[5]杜纪坤,黄青阳,李守华,等.脂蛋白脂酶基因HindⅢ酶切多态性与2型糖尿病的关联研究[J].遗传,2007,29(8):929-933.
[6]莫艳萍,张彦,张红星,等.ACE基因多态性与云南彝族原发性高血压的相关性研究[J].昆明医学院学报,2009,(9):67-72.
[7]Rigat B,Hubert C,Alhenc-Gelas F,et al.An insertion/de1etion polymorphism in the angiotensin I-converting enzyme gene accounting for half the variance of serum enzyme levels[J].J Clin Invest,1990,86(4):1343-1346.
[8]买买提明·沙比尔.维吾尔医诊断学[M].乌鲁木齐:新疆科技卫生出版社,1993:35-148.
[9]Rigat B,Hubert C,Corvol P,et al.PCR detection of the insertion/deletionp olymorphism of the Human angiotensin converting enzymegene(DCPI)[J].Nucleic Acid Res,1992,(20):1433.
[10]库热西江·托乎提,阿不都热依木·玉苏甫,哈木拉提·吾甫尔,等.维吾尔医正常体液与异常体液分型比较研究[J].中国民族医药杂志,2004,7(3):3-4.
[11]巴吐尔·买买提明.复杂性疾病维吾尔医异常黑蛋质证的代谢组学研究[D].长沙:中南大学,2011.
[12]哈木拉提·吾甫尔.维医气质、体液论及其现代研究[M].乌鲁木齐:新疆科技卫生出版社,2003:1-50.
[13]Zhou JB,Yang JK,Lu JK,et al.Angiotensin-converting enzyme gene polymorphism is associated with type 2 diabetes:A meta-analysis[J].Mol Biol Rep,2010,37(1):67-73.
[14]Niu W,Qi Y,Gao P,et al.Angiotensin converting enzyme D allele is associated with an increased risk of type 2 diabetes:evidence from a metaanalysis[J].Endocr J,2010,57(5):431-438.
[15]Remachandran V,Ismail P,Stanslas J,et al.Association of insertion/deletion polymorphism of angiotensin-converting enzyme gene with essential hypertension and type 2 diabetes mellitus in Malaysian subjects[J].J Renin Angiotensin Aldosterone Syst,2008,9(4):208-214.
[16]刘玉华,郑小文,黄娇平,等.东莞市T2DM患者与健康人ACE基因多态性研究[J].临床心身疾病杂志,2006,12(5):324-325.
[17]Qaisar MANSOOR,Amara JAVAID,Nighat BILAL,et al.Angiotensin-converting enzyme(ACE)geneⅡgenotype protects against the development of diabetic peripheral neuropathy in type 2 diabetes mellitus[J].Journal of Diabetes,2012,(4):257-261.
[18]大华.湖北汉族人PPARγ2基因Prol2Ala和ACE基因I/D多态性与2型糖尿病的关联研究[D].武汉:华中师范大学,2008.
[19]张颖,张瑞生,何帮顺,等.血管紧张素转化酶基因多态性与2型糖尿病的相关性研究[J].医学研究生学报,2010,23(10):1062-1065.
[20]AI-Harbi EM,Farid EM,Gumaa KA,et al.Angiotensin-converting enzyme gene polymorphisms and T2DM in a case-control association study of the Bahraini population[J].Mol Cell Biochem,2011,350(4):119-125.
[2]周青.2型糖尿病的相关基因多态性的研究进展[J].铜陵职业技术学院学报,2008,(1):49-52.
[3]郭明秋.ACE基因和AT1R基因多态性与糖尿病相关性的研究[D].青岛:青岛大学,2006.
[4]黄青阳,程孟荣,姬森林.2型糖尿病易感基因的连锁和关联研究(英文)[J].遗传学报,2006,(7):3-19.
[5]杜纪坤,黄青阳,李守华,等.脂蛋白脂酶基因HindⅢ酶切多态性与2型糖尿病的关联研究[J].遗传,2007,29(8):929-933.
[6]莫艳萍,张彦,张红星,等.ACE基因多态性与云南彝族原发性高血压的相关性研究[J].昆明医学院学报,2009,(9):67-72.
[7]Rigat B,Hubert C,Alhenc-Gelas F,et al.An insertion/de1etion polymorphism in the angiotensin I-converting enzyme gene accounting for half the variance of serum enzyme levels[J].J Clin Invest,1990,86(4):1343-1346.
[8]买买提明·沙比尔.维吾尔医诊断学[M].乌鲁木齐:新疆科技卫生出版社,1993:35-148.
[9]Rigat B,Hubert C,Corvol P,et al.PCR detection of the insertion/deletionp olymorphism of the Human angiotensin converting enzymegene(DCPI)[J].Nucleic Acid Res,1992,(20):1433.
[10]库热西江·托乎提,阿不都热依木·玉苏甫,哈木拉提·吾甫尔,等.维吾尔医正常体液与异常体液分型比较研究[J].中国民族医药杂志,2004,7(3):3-4.
[11]巴吐尔·买买提明.复杂性疾病维吾尔医异常黑蛋质证的代谢组学研究[D].长沙:中南大学,2011.
[12]哈木拉提·吾甫尔.维医气质、体液论及其现代研究[M].乌鲁木齐:新疆科技卫生出版社,2003:1-50.
[13]Zhou JB,Yang JK,Lu JK,et al.Angiotensin-converting enzyme gene polymorphism is associated with type 2 diabetes:A meta-analysis[J].Mol Biol Rep,2010,37(1):67-73.
[14]Niu W,Qi Y,Gao P,et al.Angiotensin converting enzyme D allele is associated with an increased risk of type 2 diabetes:evidence from a metaanalysis[J].Endocr J,2010,57(5):431-438.
[15]Remachandran V,Ismail P,Stanslas J,et al.Association of insertion/deletion polymorphism of angiotensin-converting enzyme gene with essential hypertension and type 2 diabetes mellitus in Malaysian subjects[J].J Renin Angiotensin Aldosterone Syst,2008,9(4):208-214.
[16]刘玉华,郑小文,黄娇平,等.东莞市T2DM患者与健康人ACE基因多态性研究[J].临床心身疾病杂志,2006,12(5):324-325.
[17]Qaisar MANSOOR,Amara JAVAID,Nighat BILAL,et al.Angiotensin-converting enzyme(ACE)geneⅡgenotype protects against the development of diabetic peripheral neuropathy in type 2 diabetes mellitus[J].Journal of Diabetes,2012,(4):257-261.
[18]大华.湖北汉族人PPARγ2基因Prol2Ala和ACE基因I/D多态性与2型糖尿病的关联研究[D].武汉:华中师范大学,2008.
[19]张颖,张瑞生,何帮顺,等.血管紧张素转化酶基因多态性与2型糖尿病的相关性研究[J].医学研究生学报,2010,23(10):1062-1065.
[20]AI-Harbi EM,Farid EM,Gumaa KA,et al.Angiotensin-converting enzyme gene polymorphisms and T2DM in a case-control association study of the Bahraini population[J].Mol Cell Biochem,2011,350(4):119-125.