石菖蒲超临界萃取物对抑郁模型大鼠行为学的影响
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摘要
目的探讨石菖蒲超临界萃取物对抑郁模型大鼠行为学的影响。方法 80只造模成功大鼠根据实验处理方法不同分为模型组、低剂量组、中剂量组和高剂量组,每组20只,分别按0. 05 g/100 g、0. 10 g/100 g和0. 15 g/100 g的剂量对抑郁型大鼠进行药液灌胃治疗,以20只正常大鼠为空白组,对各组糖水偏爱度、强迫游泳不动时间和旷场运动得分进行观察和评分。结果实验第1天,模型组和低、中、高剂量组大鼠糖水偏爱度显著低于空白组[(55. 1±3. 6)%、(54. 7±2. 5)%、(55. 8±3. 4)%、(54. 9±2. 8)%比(80. 1±2. 6)%](P <0. 05),在实验第28天,低、中、高剂量组抑郁大鼠糖水偏爱度高于模型组[(62. 0±4. 5)%、(72. 5±4. 9)%、(77. 8±3. 8)%比(54. 8±3. 2)%](P <0. 05),中剂量组高于低剂量组(P <0. 05)。实验第28天,低、中、高剂量组游泳不动时间少于模型组[(112. 0±5. 4) s、(91. 8±3. 6) s、(78. 4±4. 9) s比(142. 2±4. 8) s](P <0. 05),且低、中、高剂量组逐渐降低;低、中、高剂量组水平和垂直得分均高于模型组(P <0. 05)。结论石菖蒲超临界萃取物可以有效改善抑郁型大鼠的行为学的病征。
Objective To study the effect of supercritical extract of Acorus tatarinowii on behavior of depression model rats. Methods A total of 80 successful model rats were divided into a model group,a low dose group,a middle dose group and a high dose group,20 rats each group. In the experiment,normal rats as blank group,and 0. 05 g/100 g,0. 10 g/100 g and 0. 15 g/100 g liquid medicine were given to the depressed rats through intragastric administration. The behavioral indexes such as preference for sugar and water,immobility time of forced swimming and scores of open-field sports were observed and evaluated. Results On the first day of the experiment,the glucose-water preference in the model group and in the low,middle and high dose groups was significantly lower than that in the blank group [( 55. 1 ± 3. 6) %,( 54. 7 ±2. 5) %,( 55. 8 ± 3. 4) %,( 54. 9 ± 2. 8) % vs( 80. 1 ± 2. 6) % ]( P < 0. 05). On the 28 th day of the experiment,the preference of glucose and water in the low,middle and highdose groups was higher than that in the model group[( 62. 0 ±4. 5) %,( 72. 5 ± 4. 9) %,( 77. 8 ± 3. 8) % vs( 54. 8 ± 3. 2) % ]( P < 0. 05). On the 28 th day of experiment,the swimming immobility time in the low,middle and high dose groups was shorter than that in the model group [( 112. 0 ± 5. 4) s,( 91. 8 ± 3. 6) s,( 78. 4 ± 4. 9) s vs( 142. 2 ± 4. 8) s]( P < 0. 05); The horizontal and vertical scores in the low,middle and high dose groups were higher than those in the model group( P < 0. 05). Conclusion Acorus tatarinowii supercritical extract can effectively improve the behavioral symptoms of depressive rats.
Objective To study the effect of supercritical extract of Acorus tatarinowii on behavior of depression model rats. Methods A total of 80 successful model rats were divided into a model group,a low dose group,a middle dose group and a high dose group,20 rats each group. In the experiment,normal rats as blank group,and 0. 05 g/100 g,0. 10 g/100 g and 0. 15 g/100 g liquid medicine were given to the depressed rats through intragastric administration. The behavioral indexes such as preference for sugar and water,immobility time of forced swimming and scores of open-field sports were observed and evaluated. Results On the first day of the experiment,the glucose-water preference in the model group and in the low,middle and high dose groups was significantly lower than that in the blank group [( 55. 1 ± 3. 6) %,( 54. 7 ±2. 5) %,( 55. 8 ± 3. 4) %,( 54. 9 ± 2. 8) % vs( 80. 1 ± 2. 6) % ]( P < 0. 05). On the 28 th day of the experiment,the preference of glucose and water in the low,middle and highdose groups was higher than that in the model group[( 62. 0 ±4. 5) %,( 72. 5 ± 4. 9) %,( 77. 8 ± 3. 8) % vs( 54. 8 ± 3. 2) % ]( P < 0. 05). On the 28 th day of experiment,the swimming immobility time in the low,middle and high dose groups was shorter than that in the model group [( 112. 0 ± 5. 4) s,( 91. 8 ± 3. 6) s,( 78. 4 ± 4. 9) s vs( 142. 2 ± 4. 8) s]( P < 0. 05); The horizontal and vertical scores in the low,middle and high dose groups were higher than those in the model group( P < 0. 05). Conclusion Acorus tatarinowii supercritical extract can effectively improve the behavioral symptoms of depressive rats.
引文
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[15] Li T, Li J, Liu B. Screening of the antidepressant-like effect of the traditional Chinese medicinal formula Si-Ni-San and their possible mechanism of action in mice[J]. Pharmacognosy Res,2013,5(1):36-42.
[16] Zhou X,Liu M,Yan J. Antidepressant-like effect of the extracted of Kai Xin San, a traditional Chinese herbal prescription,is explained by modulation of the central monoaminergic neurotransmitter system in mouse[J]. J Ethnopharmacol, 2012, 139(2):422-428.
[17] Jia M,Li C,Zheng Y. Leonurine Exerts Antidepressant-Like Effects in the Chronic Mild Stress-Induced Depression Model in Mice by Inhibiting Neuroinflammation[J]. Inter J Neuro,2017,20(11):886-895.
[18] Martin AL, Brown RE. The lonely mouse:Verification of a separation-induced model of depression in female mice[J]. Behav Brain Res,2010,207(l):200-207.
[19]朱彩霞.β-细辛醚对抑郁模型小鼠行为学及单胺类神经递质的影响[D].广州:广州中医药大学,2017.
[20]林晓春,李云鹏,卞艳芳.大鼠旷场实验指标检测及参考值的探讨[J].毒理学,2010,24(3):224-225.
[21] Qi X,Liu W,Li J. Fluoxetine increases the activity of the ERKCREB signal system and alleviates file depressive-like behavior in rats exposed to chronic forced swim stress[J]. Neuro Dis,2008,31(2):278-285.
[2] Croy I,Symmank A,Schellong J. Olfaction as a marker for depression in humans[J]. J Neuro,2016,160(4):1-8.
[3] Hamet P,Tremblay J. Genetics and genomics of depression[J].Metabolism,2005,54(5):10-45.
[4] Rafieian-Kopaei M,Shirzad H, Sarrafchi A. Autism:Pathophysiology and Promising Herbal Remedies[J]. Curr Pharm Des,2016,22(3):277-285.
[5] Willner P,To well A,Sampson D. Reduction of sucrose preference by chronic unpredictable mild stress, and its restoration by a tricyclic antidepressant[J]. Psycho, 1987,93(3):358-364.
[6] Russo E,LeoA,Crupi R. Everolimus improves memory and learning while worsening depressive and anxiety-like behavior in an animal model of depression[J]. Psychopharmacology,2016,78(3):1-10.
[7] Qiao H,An SC,Xu C. Role of proBDNF and BDNF in dendritic spine plasticity and depressive-like behaviors induced by an animal model of depression[J]. Brain Res,2017,1663(1):29-37.
[8]王建醒,周丽,徐华锋.慢性应激大鼠抑郁模型的建立及其评价[J].齐齐哈尔医学院学报,2006,27(6):644-646.
[9] Shen Y,Guan S, Ge H. Effects of palmatine on rats with comorbidity of diabetic neuropathic pain and depression[J]. Brain Res Bull,2018,139(1):56-66.
[10] Nair A, Vaidya V. Cyclic AMP response element binding proteinand brain-derived neurotrophic factor:Molecules that modulate our mood[J]. J Biosci,2006,31(3):423-434.
[11] Gao Y, Ma J, Tang J. White matter atrophy and myelinated fiber disruption in a rat model of depression[J]. J Comp Neurol,2017,525(8):1922-1933.
[12] Li G, Lei N, Long J. The effects of acanthopanacis senticosi pill on the expression of TH and TPH in hippocamp of depressed rats[J].Pro Modern Bio,2012,12(6):1078-1080.
[13] Wu F, Li H, Zhao L. Protective effects of aqueous extract from Acanthopanax senticosus against corticosterone-induced neurotoxicity in PC12 cells[J]. J Ethnopharmacol,2013,148(3):861-868.
[14] Khan M,Ostadhadi S,Zolfaghari S. The involvement of NMDA receptor/NO/cGMP pathway in the antidepressant like effects of baclofen in mouse force swimming test.[J]. Neurosci Lett,2016,612(3):52-61.
[15] Li T, Li J, Liu B. Screening of the antidepressant-like effect of the traditional Chinese medicinal formula Si-Ni-San and their possible mechanism of action in mice[J]. Pharmacognosy Res,2013,5(1):36-42.
[16] Zhou X,Liu M,Yan J. Antidepressant-like effect of the extracted of Kai Xin San, a traditional Chinese herbal prescription,is explained by modulation of the central monoaminergic neurotransmitter system in mouse[J]. J Ethnopharmacol, 2012, 139(2):422-428.
[17] Jia M,Li C,Zheng Y. Leonurine Exerts Antidepressant-Like Effects in the Chronic Mild Stress-Induced Depression Model in Mice by Inhibiting Neuroinflammation[J]. Inter J Neuro,2017,20(11):886-895.
[18] Martin AL, Brown RE. The lonely mouse:Verification of a separation-induced model of depression in female mice[J]. Behav Brain Res,2010,207(l):200-207.
[19]朱彩霞.β-细辛醚对抑郁模型小鼠行为学及单胺类神经递质的影响[D].广州:广州中医药大学,2017.
[20]林晓春,李云鹏,卞艳芳.大鼠旷场实验指标检测及参考值的探讨[J].毒理学,2010,24(3):224-225.
[21] Qi X,Liu W,Li J. Fluoxetine increases the activity of the ERKCREB signal system and alleviates file depressive-like behavior in rats exposed to chronic forced swim stress[J]. Neuro Dis,2008,31(2):278-285.