两种不同粒径二妙丸制剂药效对比研究
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摘要
目的:观察两种不同粒径二妙丸对佐剂性关节炎模型(AA)大鼠的改善作用及其免疫调节功能的影响。方法:将70只大鼠随机分为七组:空白组、模型组、阳性组、雷公藤多苷片组、二妙丸传统制剂与超微制剂低剂量和高剂量组,建立大鼠佐剂性关节炎模型(AA),考察二妙丸超微制剂与传统制剂对AA大鼠体重、足趾容积、脏器指数(脾脏、胸腺指数)、血清炎症因子(SOD、MDA、NO)及细胞因子IL-1β、IL-6的影响。结果:二妙丸超微制剂和传统制剂均可改善AA大鼠的足肿胀状况及炎症反应;能够升高血清中SOD含量,降低NO、MDA、IL-1β、IL-6含量;降低胸腺指数和脾脏指数;二妙丸超微制剂较其传统制剂最小有效剂量降低。结论:通过超微处理能够明显提高二妙丸的药效。
Objective:To observe the influence of two kinds of Er Miao Pill improvement and immune functions to model of adjuvant arthritis(AA)rats.Methods:Seventy rats were divided into 7 groups:blank group,model group,positive group,Er Miao Pill of traditional and ultro-micr of low-dose and high dose group,set up model of adjuvant arthritis(AA)rat,The volume of RA rats toes,organ index(spleen and thymus index),serum inflammation factors(SOD,MDA,NO,IL-1β,IL-6)were investigated to make clear the pharmacodynamic action and mechanism of ultrafine powder.Results:Both Er Miao Pill of traditional and ultro-micr can inhibit foot swelling situation of AA rats;rise SOD activity,reduced the MDA,NO,IL-1β,IL-6 content in serum,reduced thymus index and spleen index,and the effect of ultrafine powder was better than coarse powder when the dose was equal.Conclusion:It can improve drug action obviously by ultro-micr process to Er Miao Pill.
Objective:To observe the influence of two kinds of Er Miao Pill improvement and immune functions to model of adjuvant arthritis(AA)rats.Methods:Seventy rats were divided into 7 groups:blank group,model group,positive group,Er Miao Pill of traditional and ultro-micr of low-dose and high dose group,set up model of adjuvant arthritis(AA)rat,The volume of RA rats toes,organ index(spleen and thymus index),serum inflammation factors(SOD,MDA,NO,IL-1β,IL-6)were investigated to make clear the pharmacodynamic action and mechanism of ultrafine powder.Results:Both Er Miao Pill of traditional and ultro-micr can inhibit foot swelling situation of AA rats;rise SOD activity,reduced the MDA,NO,IL-1β,IL-6 content in serum,reduced thymus index and spleen index,and the effect of ultrafine powder was better than coarse powder when the dose was equal.Conclusion:It can improve drug action obviously by ultro-micr process to Er Miao Pill.
引文
[1]尹莲.二妙丸系列类方作用规律的相关性探讨[J].中国实验方剂学杂志,2007,13(8):94-96.
[2]张静,李佳伟,张永静.超微粉碎技术在中药中的研究进展[J].包头医学院学报,2015,31(5):150-153.
[3]麻晓雪,李娟,李顺祥,等.铁皮石斛超微饮片与细粉颗粒体外溶出度比较[J].湖南中医药大学学报,2013,33(7):34-37.
[4]张秀丽,雷昌,孟盼,等.四妙丸超微制剂与传统制剂对佐剂性关节炎的改善作用及免疫调节比较研究[J].亚太传统医药,2016,12(15):15-18.
[5]杨蕙,王宇红,张秀丽,等.马钱子超微粉对类风湿性关节炎模型大鼠的量效关系参数研究[J].中国中医急症,2012,21(12):1942-1944.
[6]褚春民,陈放,张洪泉,等.薯蓣皂苷对胶原性关节炎大鼠的免疫调节[J].实用临床医药杂志,2013,17(1):5-9.
[7]曾平,梁景超,秦刚,等.壮医通路酒对佐剂性关节炎大鼠的治疗效果及机制研究[J].广西医科大学学报,2013,30(6):863-866.
[8]史学军,张立群.二妙丸与四妙丸的组方及临床合理应用[J].药物不良反应杂志,2005,7(6):427-429.
[2]张静,李佳伟,张永静.超微粉碎技术在中药中的研究进展[J].包头医学院学报,2015,31(5):150-153.
[3]麻晓雪,李娟,李顺祥,等.铁皮石斛超微饮片与细粉颗粒体外溶出度比较[J].湖南中医药大学学报,2013,33(7):34-37.
[4]张秀丽,雷昌,孟盼,等.四妙丸超微制剂与传统制剂对佐剂性关节炎的改善作用及免疫调节比较研究[J].亚太传统医药,2016,12(15):15-18.
[5]杨蕙,王宇红,张秀丽,等.马钱子超微粉对类风湿性关节炎模型大鼠的量效关系参数研究[J].中国中医急症,2012,21(12):1942-1944.
[6]褚春民,陈放,张洪泉,等.薯蓣皂苷对胶原性关节炎大鼠的免疫调节[J].实用临床医药杂志,2013,17(1):5-9.
[7]曾平,梁景超,秦刚,等.壮医通路酒对佐剂性关节炎大鼠的治疗效果及机制研究[J].广西医科大学学报,2013,30(6):863-866.
[8]史学军,张立群.二妙丸与四妙丸的组方及临床合理应用[J].药物不良反应杂志,2005,7(6):427-429.