HPLC双波长切换法测定二妙丸中盐酸小檗碱、苍术素含量
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摘要
目的:建立HPLC双波长切换法对二妙丸中盐酸小檗碱、苍术素的含量测定,该方法有利于二妙丸的含量测定及质量控制。方法:采用反相高效液相色谱法;以0.02mol/L磷酸二氢钾(用磷酸调PH3.0)-乙腈(色谱纯)流动相梯度洗脱;流速:1.0m L/min;柱温:30℃;检测波长:270nm(0~10min,盐酸小檗碱)、340nm(10~20min,苍术素);结果:HPLC双波长切换法可同时测定二妙丸中盐酸小檗碱、苍术素的含量,盐酸小檗碱和苍术素分别在14.63~292.5μg/m L、0.54~10.75μg/m L和峰面积呈良好的线性关系,平均加样回收率(N=6)分别为99.8196%、99.2808%。RSD分别为0.6163%、2.1763%。结论:该方法准确、可靠、专属性强,为二妙丸的质量控制提供参考。
Objective: To establish HPLC dual wavelength switching method to determine the contents of berberine hydrochloride and atractylodin in Ermiao powder,which can be beneficial for content determination and quality control of Ermiao powder. Methods: Using RP-HPLC method,0. 02mol/L potassium dihydrogen phosphate(adjusted to PH3. 0 with phosphoric acid)---acetonitrile(HPLC grade) gradient elution; flow rate: 1.0m L/min; column temperature: 30℃; detection wavelength: 270nm(0 ~ 10 min,berberine hydrochloride),340nm(10 ~ 20 min,atractylodin). Results: The HPLC dual wavelength switching method can simultaneously determinate the contents of berberine hydrochloride and atractylodin in Ermiao powder. The linear rangs are 292. 5 ~ 14. 63μg/m L and 10. 75 ~ 0. 54 μg/m L respectively. The average recovery rate(N = 6) are99. 8196%(RSD = 0. 6163%) and 99. 2808%(RSD = 2. 1763%) respectively. Conclusion: This method is accurate,reliable,exclusive,which can provide reference for the quality control of Ermiao powder.
Objective: To establish HPLC dual wavelength switching method to determine the contents of berberine hydrochloride and atractylodin in Ermiao powder,which can be beneficial for content determination and quality control of Ermiao powder. Methods: Using RP-HPLC method,0. 02mol/L potassium dihydrogen phosphate(adjusted to PH3. 0 with phosphoric acid)---acetonitrile(HPLC grade) gradient elution; flow rate: 1.0m L/min; column temperature: 30℃; detection wavelength: 270nm(0 ~ 10 min,berberine hydrochloride),340nm(10 ~ 20 min,atractylodin). Results: The HPLC dual wavelength switching method can simultaneously determinate the contents of berberine hydrochloride and atractylodin in Ermiao powder. The linear rangs are 292. 5 ~ 14. 63μg/m L and 10. 75 ~ 0. 54 μg/m L respectively. The average recovery rate(N = 6) are99. 8196%(RSD = 0. 6163%) and 99. 2808%(RSD = 2. 1763%) respectively. Conclusion: This method is accurate,reliable,exclusive,which can provide reference for the quality control of Ermiao powder.
引文
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[2]中华人民共和国药典一部[S].北京:中国医药科技出版社,2015:438-439.
[3]潘超,张莉,王玉.HPLC法同时测定二妙丸中黄柏碱、木兰花碱、药根碱、盐酸巴马汀和盐酸小檗碱的含量[J].中国生化药物杂志,2012,33(4):361-364.
[4]陈炎明,侴桂新,王峥涛.反相高效液相色谱法同时测定苍术中的两种聚乙炔类化合物[J].色谱,2007,25(1):84-87.
[5]李宁,王拥军,周泉等.二妙散及其衍生方在骨伤科的应用与研究[J].中国中医骨伤科杂志,2011,19(11):69-71.
[6]张蓓蕾,夏醒醒,陈勤.HPLC测定二妙丸中盐酸小檗碱的含量[J].中成药,2008,30(1):152-154.
[7]付梅红,朱东海,方婧.苍术的化学、分子生药学和药理学研究进展[J].2009,34(20):2669-2672.
[8]李欣.二妙丸类方抗湿热证痛风物质基础相关性研究[D].南京:南京中医药大学,2009:1-119.
[9]张杨,张振秋,王海波,等.二妙丸多指标化学成分质量研究[J].中成药,2009,31(4):562-566.
[10]张莉,潘超.高效液相色谱法同时测定二妙丸中盐酸小檗碱和黄柏碱的含量[J].中国新药杂志,2011,20(22):2262-2264.
[11]郭金鹏.苍术挥发油化学成分及其抗菌活性的研究[J].时珍国医国药,2011,22(3):566-568.